Protective Effects of Different Egg Yolk Sources on Cryopreservation of Scaly Carp (Cyprinus carpio) Sperm

Year 2022, , 393 - 402, 01.09.2022

Hasan Avlar , Yusuf Bozkurt

https://doi.org/10.22392/actaquatr.1085283

Abstract

Egg yolk is one of the most widely used cryoprotective components of extenders, especially for the cryopreservation of mammalian species’ sperm cells. However, there is a lack of information regarding their efficacy in cryopreservation of fish sperm cells. Thus, the objective of this experiment was to compare the effectiveness of egg yolk from different avian species (duck, goose, and chicken) on post-thaw quality and fertilization ability of scaly carp (Cyprinus carpio) semen following cryopreservation. Sperm samples diluted with the sucrose-based extender at the ratio of 1:10 were supplemented with 10, 15, and 20% ratios of different avian egg yolks. In the control group, sperm samples were diluted with the sucrose-based extender, without egg yolk. Following dilution, sperm samples were equilibrated at +4⁰C for 10 min and aspirated into 0.25-ml straws. Then, sperm samples were frozen 3 cm above the liquid nitrogen (LN2) surface and plunged directly into the LN2. The frozen sperm cells were thawed in a water bath at 35 ⁰C for 30 s and fertilization was carried out using a 1x105 spermatozoa/egg ratio. Based on the results, supplementation of sperm cells with 20 % duck egg yolk in a sucrose-based extender exhibited the best post-thaw progressive motility (67.8 ±1.24%), progressive motility duration (32.6 ±1.45 s), viability (82.4 ±1.36%), and fertility (92.6 ±1.28%) compared to the control group (P<0.05). The results of the experiment showed that duck egg yolk could be used as an alternative instead of chicken egg yolk in a sucrose-based extender for the cryopreservation of scaly carp sperm.

Keywords

cryopreservation, egg yolk, fertility, extender, sperm

Thanks

The authors thank to the staff of aquaculture production station of Directorate of Agriculture and Forestry in Şanlıurfa, (Turkey) for their technical assistance.

Farklı Yumurta Sarısı Kaynaklarının Pullu Sazan (Cyprinus carpio) Spermasının Kriyoprezervasyonu Üzerine Koruyucu Etkileri

Abstract

Özet: Yumurta sarısı, özellikle memeli türlerine ait sperm hücrelerinin dondurularak saklanmasında kullanılan sulandırıcıların en yaygın kriyoprotektif bileşenlerinden biridir. Dolayısıyla, balık sperm hücrelerinin dondurularak saklanmasındaki etkinlikleri hakkında bilgi eksikliği bulunmaktadır. Bu nedenle bu çalışmanın amacı, farklı kanatlı türlerine (ördek, kaz ve tavuk) ait yumurta sarılarının, kriyoprezervasyonu takiben pullu sazan (Cyprinus carpio) spermasının çözüm sonu kalite ve fertilizasyon yeteneği üzerindeki etkinliklerinin karşılaştırılmasıydı. Sukroz bazlı sulandırıcı ile 1:10 oranında dilüe edilen sperm örneklerine %10, 15 ve %20 oranlarında farklı kanatlı yumurtası sarıları ilave edildi. Kontrol grubunda ise sperm örnekleri, yumurta sarısı içermeyen sukroz bazlı sulandırıcı ile dilüe edildi. Dilüsyon işlemini takiben, sperm örnekleri +4⁰C'de 10 dakika ekilibere edilerek 0,25 ml'lik payetlere çekildi. Daha sonra sperm hücreleri sıvı nitrojen (LN2) yüzeyinin 3 cm üzerinde dondurularak doğrudan sıvı azota (LN2) aktarıldı. Dondurulan sperm hücreleri 35 ⁰C su banyosunda 30 sn süre ile çözdürüldü ve 1x105 spermatozoa/yumurta oranı kullanılarak fertilizasyon işlemi gerçekleştirildi. Sonuçlara göre, sperm hücreleri sukroz bazlı sulandırıcıda %20 ördek yumurtası sarısı ile takviye edildiğinde, kontrol grubu ile karşılaştırıldığında en iyi çözüm sonu progresif motilite (%67,8 ±1,24), progresif motilite süresi (32,6 ±1,45 s), canlılık (%82,4 ±1,36) ve fertilite (%92,6 ±1,28) değerleri elde edilmiştir (P<0.05). Çalışmanın sonuçları, pullu sazan sperminin dondurularak saklanmasında sukroz bazlı sulandırıcı kullanıldğında, tavuk yumurtası sarısı yerine ördek yumurtası sarısının alternatif olarak kullanılabileceğini göstermiştir. 

Keywords

kriyoprezervasyon, yumurta sarısı, fertilite, sulandırıcı, sperm

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