First Report of Candida sp. from Rainbow Trout (Oncorhynchus mykiss, Walbaum) Fry in Turkey

Year 2019, Volume: 15 Issue: 1, 55 - 59, 01.03.2019

Seçil Metin , Behire İşıl Didinen , Ayşegül Kubilay , Bülent Baş , Pınar Yıldırım , Tom Wıklund

https://doi.org/10.22392/egirdir.437661

Cited By: 1

Abstract

In this study, we isolated Candida
sp. for the first time from fry of rainbow trout (Oncorhynchus mykiss) in Turkey. Candida species caused disease in fry (average 0.2 g) which
was observed at 10°C water temperature in February 2016. Due to yeast
accumulation on gills, whitish areas was seen and fusion of secondary lamella
in gills were observed under microscope. The microbiological samples of kidney,
spleen and gills were streaked onto trypticase soy agar (TSA) and tryptone
yeast extract salts (TYES) agar plates
and incubated at 22°C in TSA and 18°C in TYES agar for 48 h. Thirteen isolates
were obtained and Gram staining was performed. The all of the isolates were determined as yeast cell
based on cellular morphology by using light microscopy. The identification was performed by ITS (Internal transcribed spacer) region
sequence analysis and sequence data demonstrated that representative isolate
D11 belonged to genus Candida. The
yeast infection might have seen in early development stages (larvae and fry)
due to the intensive antibiotic use against rainbow trout fry syndrome (RTFS)
disease.

Keywords

Candida sp., rainbow trout, Oncorhynchus mykiss, identification, sequence analysis

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