Nemrut Krater Gölünden (Bitlis/Türkiye) İzole Edilen Chlorella sp. Mikroalginde H2O2 Uygulaması ile Triaçilgliserol Üretiminin Artırılması

Year 2019, Volume: 15 Issue: 3, 280 - 288, 15.09.2019

Emine Selçuk Turgay Çakmak

https://doi.org/10.22392/actaquatr.508780

Abstract

Bu
çalışmada Nemrut volkanik gölünden izole edilen bir Chlorella sp. mikroalginde oksitatif stresin düzeyine bağlı olarak triaçilgliserol
miktarında ve formasyonundaki değişiklikler incelenmiştir. Bu amaçla
mikroalglerde oksidatif stresi uyarmak için büyüme ortamlarına 1µM, 5µM, ve 20µM
yoğunluklarda Hidrojen peroksit (H₂O₂) uygulanmıştır. Hidrojen
peroksituygulaması ilk günden itibaren büyümede önemli düzeyde
baskılanmaya sebep olmuştur. Uygulamanın ilk 5 gününde büyümedeki baskılanma
artmış, takip eden günlerde 1µM ve 5µM H₂O₂ uygulanan
gruplarda büyüme yeniden gelişmiş ve baskılanma kalkmıştır. Mikroalglerin H₂O₂
içerikleri, ugulanan H₂O₂ yoğunluğu ile orantılı
şekilde artmıştır. Mikroalglerin klorofil ve karotenoid içerikleri H₂O₂
uygulanan gruplarda konsantrasyon ile doğru orantılı olarak ilk 24 saatte
artmış, daha sonra da lineer olarak azalmıştır. Mikroalglerin triaçilgliserol
içerikleri özellikle 5uM H₂O₂ uygulamasına cevapta ilk 24
saatte yaklaşık olarak 2.1 kat artış ile en yüksek düzeyde gerçekleşmiştir. Floresans
görüntüleme ile elde edilen sitoplazmik lipid cisimi üretimindeki artış da bu
veriyi desteklemiştir. Böylece, bu çalışma ile elde edilen sonuçlar kısa süreli
ve düşük yoğunlukta H₂O₂ uygulamasının mikroalglerden
biyodizel hammaddesi olan triaçilgliserol üretiminde kullanılabilecek bir
yaklaşım olduğunu göstermektedir.

Keywords

Chlorella, H2O2, nötral lipid, oksidatif stres, triaçilgliserol

Enhancement of Triacylglycerol Production via H2O2 Application in Chlorella sp. Isolated from Nemrut Crater Lake (Bitlis/Turkey)

Abstract

In this study, oxidative stress depended
changes in lipid production and formation in Chlorella sp. was analyzed. The strain was isolated from Lake
Nemrut which is a volcanic lake located in Bitlis province. In order to induce
oxidative stress in microalgae 1µM, 5µM, and 20µM hydrogen peroxide (H₂O₂)
were added into the growth media. Hydrogen peroxide application caused a
significant decrease in growth starting from the first day of incubation.
Following decrease of the growth for first five-days, microalgae started to
grow up as a response to 1µM and 5µM H₂O₂ application.
The H₂O₂ contents of microalgae increased in proportion
to the applied H₂O₂ concentation. Total chlorophyll and
carotenoid contents showed a gradual increase during first 24 hours of H₂O₂
application, and then a linear decrease was followed afterwards. A maximum of
2.1 fold increase in triacylclygerole content of microalgae was recorded after
24 hours of 5uM H₂O₂ aplication.  Fluorescence imaging of cytoplasmic lipid
bodies support this datum. Thus, results of this study showed that short-term
and low-density H₂O₂ application might be used as a
potential approach to induce microalgal triacylglycerol production as a
biodiesel feedstock.

Keywords

Chlorella, H2O2, neutral lipid

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