Karanfil Yağının Japon Balığının (Carassius auratus) Anestezisinde Optimal Konsantrasyonu ve Balığın Taşınmasında Stoklama Yoğunluğuna Etkisi

Year 2022, Volume: 18 Issue: 2, 147 - 158, 01.06.2022

Mahmut Yanar Kazım Ergin Gürkan Ece Evliyaoğlu

https://doi.org/10.22392/actaquatr.923939

Abstract

Bu çalışmada, karanfil yağının ortalama ağırlığı 4,16±0,61 g olan japon balığının (Carassius auratus) derin anestezi (30 dk) ve taşınması (15 s) için etkili olan konsantrasyonları tespit edilmiştir. Ayrıca karanfil yağının balıkların 15 s süreli taşınmasında stoklama yoğunluğu üzerindeki etkisi belirlenmiştir. Karanfil yağının konsantrasyonu ve uygulama süresine bağlı olarak anesteziye giriş süresi 2 ile 11 dk, kendine gelme süresi ise 5 ile 17 dk arasında değişmiştir. Balıkların solunum sayısı anestezi içermeyen suda 73/dk iken, karanfil yağı uygulanmış olanlarda konsantrasyon ve uygulama süresine bağlı olarak 104/dk’ya kadar çıkmıştır. Karafil yağının 30 dk sürede hafif anestezi için 6-12 µL/L, derin anestezi için 18-24 µL/L; 15 s sürede hafif anestezi için 6-9 µL/L, derin anestezi için 12-18 µL/L konsantrasyonları güvenle kullanılabilir. Karanfil yağının toksik konsantrasyonları 30 dk uygulama için 42 µL/L, 15 s uygulama için 21 µL/L olarak saptanmıştır. Balıkların 15 s süreli taşınmasında taşıma suyuna karanfil yağı ilavesinin balığın yaşama oranı üzerine bir etkisi olmamıştır. Jüvenil japon balıklarının 3/4 oksijen basılmış naylon torbalarda 15 s süre ile taşınmasında, %98-99 yaşama oranı ile en uygun stoklama oranları 80 veya 100 adet/L’dir.

Keywords

japon balığı, karanfil yağı, anestezi, stoklama yoğunluğu, taşıma

Supporting Institution

Çukurova Üniversitesi Bilimsel Araştırmalar Projeleri Koordinasyon Birimi

Project Number

FYL-2019-12032

Thanks

Bu çalışma yüksek lisans tezinden üretilmiştir. Bu tez çalışmasına FYL-2019-12032 nolu projeyle finansal destek sağlayan Çukurova Üniversitesi Bilimsel Araştırmalar Projeleri Koordinasyon Birimine teşekkür ederiz.

Optimal Concentration of Clove Oil in Anesthetization of Goldfish (Carassius auratus) and its Effect on Stocking Density for Fish Transportation

Abstract

In this study, the effective concentrations of clove oil for deep anesthesia (30 min) and transportation (15 h) of goldfish (Carassius auratus) with an average weight of 4.16±0.61g were determined. Besides, the effect of clove oil on stocking density in transporting fish for 15 hours was determined. Depending on the concentrations and application time of clove oil, induction time ranged from 2 to 11 min while recovery time varied between 5 and 17 min. Depending on the concentrations and application time of clove oil, the respiratory rate of fish increased from 73/min to 104/min with the addition of clove oil in the anesthetic-free water. 6-12 µL/L for light anesthesia and 18-24 µL/L for deep anesthesia at a 30 min period, 6-9 µL/L for light anesthesia and 12-18 µL/L for deep anesthesia at a 15 h period are determined concentrations of clove oil that can be used safely. The toxic concentrations of clove oil were determined as 42 µL/L for 30 min application, 21 µL/L for 15 h application. Adding clove oil to the transport water did not affect the survival rate of the fish during the 15 h transport period. When transporting juvenile goldfish in 3/4 oxygen compressed nylon bags for 15 h, the most suitable stocking rate is 80 or 100 fish/L with a 98-99% survival rate.

Keywords

goldfish, clove oil, anesthesia, stocking density, transportation

Project Number

FYL-2019-12032

References

• Abdolazizi, S., Ghaderi, E., Naghdi, N., & Kamangar, B. (2011). Effects of clove oil as an anesthetic on some hematological parameters of Carassius auratus. Aquaculture Research and Development, 2 (1), 1-3. http://dx.doi.org/10.4172/2155-9546.1000108
• Akbulut, B., Çakmak, E., Aksungur, N., & Çavdar,Y. (2011). Effect of exposure duration on time to recovery from anaesthesia of clove oil in juvenile of russian sturgeon. Turkish Journal of Fisheries and Aquatic Sciences, 11 (3), 463-467. https://doi.org/10.4194/1303-2712-v11_3_17
• Bennett, W.A., & Beitinger, T.L. (1997). Temperature tolerance of the sheepshead minnow, Cyprinodon variegatus. Copeia, (1), 77-87. https://doi.org/1447842
• Briozzo, J., Nuncez, L., Chirife, J., Herzage, L., & D’Aquino, M. (1989). Antimicrobial activity of clove oil dispersed in a concentrated sugar solution. Journal of Applied Bacteriology, 66, 69–75. https://doi.org/10.1111/j.1365-2672.1989.tb02456.x
• Bullerman, L. B., Lieu, F.Y., & Seier, S.A. (1977). Inhibition of growth and aflatoxin production by cinnamon and clove oils, cinnamic aldehyde and eugenol. Journal of Food Science, 42(4), 1107-1116. https://doi.org/10.1111/j.1365-2621.1977.tb12677.x
• Chellapan, A., Rajagopalsamy, C.B.T., & Jasmine, G.I. (2013). Effect of clove oil and benzocaine on the respiratory metabolism of angel fish Pterophyllum scalare. Indian Journal Science Technology, 6(7), 4853-4861.
• Cooke, S.J., Suski. C.D., Ostrand, K.G., Tufts, B.L., & Wahl, D.H. (2004). Behavioral and physiological assessment of low concentrations and transporting largemouth bass (Micropterus salmoides). Aquaculture, 239, 509-529. https://doi.org/10.1016/j.aquaculture.2004.06.028
• Dolezelova, P., Macova, S., Plhalova, L., Pistekova, V., & Svobodova, Z. (2011). The acute toxicity of clove oil to fish Danio rerio and Poecilia reticulata. Acta Veterinaria Brno, 80, 305–308. https://doi.org/10.2754/avb201180030305
• FAO, (2014). Ornamental fish. Food and agriculture organization of the United Nations. Fisheries and aquaculture departmant. www.fao.org/fishery/topic/13611/en (accessed 18 December 2014)
• Fischer, I.U., Unruh, G.E., & Dengler, H.J. (1990). The metabolism of eugenol in man. Xenobiotica, 20, 209–222. https://doi.org/10.3109/00498259009047156
• Ghosh, A., Mahapatra, B.K., & Datta, N.C. (2003). Ornamental fish farming-successful small scale aqua business in India. Aquaculture Asia, 8 (3), 14-16.
• Grush,J., Noakes, D.L.G., & Moccia, R.D. (2004). The Efficacy of clove oil as an anesthetic for the zebrafish, Danio rerio (Hamilton). Zebrafish, 1 (1), 46- 53. https://doi.org/10.1089/154585404774101671
• Hajek, G.J., Klyszejko, B., & Dziaman, R.(2006). The anaesthetic effect of clove oil on common carp, Cyprinus carpio L. Acta Ichthyologica Piscatoria, 36(2), 93–97.
• Hamackova, J., Sedova J.M., Pjanova S.V., & Lepicova, A.(2002). The effect 2-phenoxyethanol, clove oil and propiscin anaesthetics on perch (Perca fluviatilis) in relation to water temperature. Czech Journal of Animal Science, 46 (11), 469–473.
• Han, M.C., Sağlıyan, A., & Polat, E. (2016). Akvaryum balıklarında karanfil yağının anestezik etkisinin araştırılması, Harran Üniversitesi Veteriner Fakültesi Dergisi, 5(1), 12-17.
• Hekimoğlu, M.A., & Ergun, M. (2012). Evaluation of clove oil as anaesthetic agent in fresh water angelfish, Pterophyllum scalare. Pakistan Journal Zoology, 44(5), 1297-1300.
• Hikasa, Y., Takase, K., Ogasawara, T., & Ogasawara, S.(1986). Anaesthesia and recovery with tricain methansulfonate, eugenol and thiopental sodium in carp, Cyprinus carpio. The Japanese Journal of Veterinary Science, 48 (2), 341-351. https://doi.org/10.1292/jvms1939.48.341
• Kaiser, H., Brill,G., Cahill, J., Collett, P., Czypionka, K., Green, A., Orr, K., Pattrick, P., Scheepers, R., Stonier, T., Whitehead, M.A., & Yearsley, R. (2006). Testing clove oil as an anaesthetic for long-distance transport of live fish: the case of the Lake Victoria cichlid Haplochromis obliquidens. Journal of Applied Ichthyology, 22, 510–514. https://doi.org/10.1111/j.1439-0426.2006.00786.x
• Kamacı, H.O., Süzer, C., Çoban, D., Özdilek, G., Saka, Ş., & Fırat, K. (2009). Levrek (D. labrax) juvenillerinde tuzluluk değişimlerinin anestezi uygulamalarına olan etkileri: Karanfil yağı örneği. 15.Ulusal Su Ürünleri Sempozyumu, Rize.
• Keene, J.L., Noakes, D.L.G., Moccia, R.D., & Soto, C.G. (1998). The efficacy of clove oil as an anaesthetic for rainbow trout, Onchorhynchus mykiss (Walbaum). Aquaculture Research, 29, 89-101. https://doi.org/10.1046/j.1365-2109.1998.00927.x
• Koçak, V., & Can, E. (2019). 2-Fenoksietanol ve karanfil esansiyel yağının sarı prenses (Labidochromis caeruleus) ve ahli (Sciaenochromis fryeri) balıkları üzerine anestetik etkileri. Biyoloji Bilimleri Araştırma Dergisi, 12 (2), 13-21.
• Kolarova, J., Svobodova, Z., Nepejchalova, L., Velisek, J., & Piackova, V. (2006). Anaesthesia of fish in Czech Republic. Bulletin VURH Vodnany, 42, 105-108.
• Kramer, R.E. (1985). Antioxidants in clove. Jounal of American Oil Chemist Society, 62, 111-113. https://doi.org/10.1007/BF02541505
• Küçükosman, N. (2019). Karanfil (Eugenia caryophylatta), nane (Menta piperita) ve lavanta (Lavandula angustifolia) bitkilerinden elde edilen esansiyel yağların japon balıklarında (Carassius auratus, linnaeus, 1758) anestezik etkilerinin araştırılması ve uygun dozun belirlenmesi. Ordu üniversitesi Fen bilimleri enstitüsü, yüksek lisans tezi, Ordu.
• Marking, L.L, & Meyer, F.P. (1985). Are better anesthetics needed in fisheries? Fisheries, 10 (6): 2-5. https://doi.org/10.1577/1548-8446(1985)010<0002:ABANIF>2.0.CO;2
• Maura, A., Pino, A., & Ricci, R. (1989). Negative evidence in vivo of DNA-damaging, mutagenic and chromosomal effects of eugenol. Mutation Research Letters, 227 (2), 125-129. https://doi.org/10.1016/0165-7992(89)90008-0
• McFarland, W.N. (1959). A study of the effects of anaesthetics on the behaviour and physiology of fishes. Publication of the Institute of Marine Science, University of Texas at Austin, 6, 23-55.
• Molinero, A., & Gonzalez, J. (1995). Comparative effects of MS 222 and 2-phenoxyethanol on gilthead sea bream (Sparus aurata L.) during confinement. Comparative Biochemistry and Physiology, 111, 405-414. https://doi.org/10.1016/0300-9629(95)00037-8
• Munday, P.L., & Wilson, S.K. (1997). Comparative efficacy of clove oil and other chemicals in anaesthetization of Pomacentrus amboinensis, a coral reef fish. Journal of Biology, 51, 931-938. https://doi.org/10.1111/j.1095-8649.1997.tb01532.x
• Mylonas, C.C., Cardinalettia. G., Sigelaki, I., & Polzonetti-Magni, A. (2005). Comparative efficacy of clove oil and 2-phenoxyethanol as anesthetics in the aquaculture of european sea bass Dicentrarchus labrax and gilthead sea bream Sparus aurata at different temperatures. Aquaculture, 246(1-4), 467-481. https://doi.org/10.1016/j.aquaculture.2005.02.046
• Otay, T., Küçükgül, A., Pala, A., & Şeker, E. (2014). Sazan balıklarının anestezisinde karanfil kullanımı. Bilim ve Gençlik Dergisi, 2(1), 43-50.
• Öğretmen, F., & Gökçek, K. (2013). Comparative efficacy of three anesthetic agents on juvenile African catfish, Clarias gariepinus (Burchell, 1822). Turkish Journal of Fisheries and Aquatic Sciences, 13, 51-56. https://doi.org/10.4194/1303-2712-v13_1_07
• Pattanasiri,T., & Taparhudee,W. (2017). Acute toxicity and anaesthetic effect of clove oil and eugenol on Siamese fighting fish, Betta splendens. Aquaculture International, 25,163-175. https://doi.org/10.1007/s10499-016-0020-2
• Perdikaris, C., Nathanailides, C., Gouva, E., Gabriel, U.U., Bitchava, K., Athanasopoulou, F., Paschou, A., & Paschos, I. (2010). Size-relative effectiveness of clove oil as an anaesthetic for rainbow trout (Oncorhynchus mykiss) and goldfish (Carassius auratus Linnaeus, 1758), Acta Veterinaria Brno, 79, 481-490. https://doi.org/10.2754/avb201079030481
• Prince, A., & Powell, C. (2000). Clove oil as an anesthetic for invasive field procedures on adult rainbow trout. North American Journal of Fisheries Management, 20, 1029-1032. https://doi.org/10.1577/1548-8675(2000)020<1029:COAAAF>2.0.CO;2
• Raghavan, R., Dahanukar, N., Tlusty, M.F., Rhyne, A.L., Kumar, K.K., Molur, S., & Rosser, A.M. (2013). Uncovering an obscure trade: Threatened freswater fishes and the aquarium pet markets. Biological Conservation, 164, 158-169. https://doi.org/10.1016/j.biocon.2013.04.019
• Ross, L.G., & Ross, B.R. (2008). Anaesthetic and sedative techniques for aquatic animals. Blackwell, Oxford, 218 s.
• Siddiqui, Y.M. (1996). Effect of essential oils on the enveloped viruses: antiviral activity of oregano and clove oils on herpes simplex virus type I and Newcastle disease virus. Medical Scinces Research, 24, 185-186.
• Simoes, L.N., Lombardi, D.C., Gomide, A.T.M., & Gomes, L.C. (2011). Efficacy of clove oil as anesthetic in handling and transportation of Nile tilapia, Oreochromis niloticus (Actinopterygii: Cichlidae) juveniles. Zoologica, 28, 285-290. https://doi.org/10.1590/S1984-46702011000300001
• Soto, C.G., & Burhanuddin, (1995). Clove oil as a fish anaesthetic for measuring length and weight of rabbitfish (Siganus lineatus). Aquaculture, 136, 149-152. https://doi.org/10.1016/0044-8486(95)01051-3
• Stecchini, M.L, Sarais, I., & Giavedoni, P. (1993). Effects of essential oils on Aeromonas hydrophila in a culture medium and in cooked pork. Journal of Food Protection, 56 (5), 406-409. https://doi.org/10.4315/0362-028X-56.5.406
• Stoskopf, M. (1993). Anaesthesia. In Aquaculture for Veterinarians (Brown, L., ed.), Oxford: Pergamon Press, 161-167s.
• Summerfelt , R.C., & Smith L.S. (1990). Anesthesia, surgery and related techniques. In: Methods for Fish Biology (Ed.by C.B. Schreck & P.B Moyle), American Fisheries Society, Bethesda, MD, USA, 213-272 s.
• Şahin, A., Kankaya, E., Yılmaz, O., Türel, İ., & Öner, A.C. (2019). Anesthetic activity of clove oil (Eugenol) on the Lake Van fish (Chalcalburnus tarichi Pallas, 1811). Van Veterinary Journal, 30(3), 175-176. https://doi.org/10.36483/vanvetj.598527
• Taylor, S.D. (1999). Clove Oil: An alternative anaesthetic for aquaculture. North American Journal of Aquaculture, 61, 150-155. https://doi.org/10.1577/1548-8454(1999)061<0150:COAAAF>2.0.CO;2
• Tlusty, M.F., Rhyne, A.L., Kaufman, L., Hutchins, M., Reid, G.M., Andrews, C., Boyle, P., Hemdal, J., Mcgilvray, F., & Dowd, S. (2012). Opportunities for public aquariums to increase the sustainability of the aquatic animal trade. Zoo Biology, 32, 1-12. https://doi.org/10.1002/zoo.21019
• Tort, L., Puigcever, M., Crespo, S., & Padros, F. (2002). Cortisol and haematological response in eea bream and trout subjected to the anaestethics clove oil and 2-phenoxyethanol. Aquaculture Research, 33, 907-910. https://doi.org/10.1046/j.1365-2109.2002.00741.x
• Türkmen, G., & Çelik, İ. (2014). Türkiye'de yetiştiriciliği yapılan akvaryum balık türleri konusunda ön çalışma, Ulusal Akvaryum Balıkçılığı ve Sorunları Çalıştayı, 44-51 s. 30-31 Ekim 2015, Antalya.
• Velisek, J., Svobodova, Z., & Piackova, V. (2005a). Effetc of clove oil anaesthesia on rainbow trout (Oncorhynchus mykiss). Acta Veterinaria Brno, 74, 139-146.
• Velisek, J., Svobodova, Z., Piackova, V., Groch, L., & Nepejchalova, L. (2005b). Effects of clove oil anaesthesia on common carp (Cyprinus carpio L.). Acta Veterinaria Brno, 50 (6), 269-275.
• Waterstrat, P.R. (1999). Induction and recovery from anesthesia in channel catfish Ictalurus punctatus fingerlings exposed to clove oil. Journal of the World Aquaculture Socıety, 30 (2), 250-255. https://doi.org/10.1111/j.1749-7345.1999.tb00872.x
• Weber, R.A., Peleteiro, J.B., Garcia Martin, L.O., & Aldegunde, M. (2009). The efficacy of 2- phenoxyethanol, metomidate, clove oil and MS-222 as anaesthetic agents in the Senegalese sole (Solea senegalensis Kaup 1858). Aquaculture, 288, 147-150. https://doi.org/10.1016/j.aquaculture.2008.11.024
• Woody, C.A., Nelson, J., & Ramstad., K. (2002). Clove oil as anaesthetic for adult Sockeye salmon: field trials. Journal of Fish Biology, 60, 340-347. https://doi.org/10.1111/j.1095-8649.2002.tb00284.x
• Yanar, M., Öter, H.H., & Evliyaoğlu, E. (2020). Fenoksietanol ve açlık süresinin japon balığının (Carassius auratus) taşınmasında stok miktarına etkisi. KSU Tarım ve Doğa Dergisi, 23 (6), 1554-1560. https://doi.org/10.18016/ksutarimdoga.vi.658550
• Yoshikawa, H., Ishida, Y., Ueno, S., & Mitsuda, H. (1988). Changes in depth of anesthesia of the carp anesthetized with a constant level of CO2. Nippon Suisan Gakkaishi, 54 (3), 457-462. https://doi.org/10.2331/suisan.54.457