In vitro antibacterial activity of Origanum onites and Mentha spicata subs tomentosa essential oil nanoemulsions against bacterial fish pathogens

Year 2022, Volume: 18 Issue: 4, 495 - 504, 01.12.2022

Mevlüt Nazıroğlu Öznur Diler Öznur Özil Abdullah Diler

https://doi.org/10.22392/actaquatr.1145109

Cited By: 1

Abstract

The current study aimed to determine the antimicrobial activities of two different aromatic plants (Origanum onites, Mentha spicata subs. tomentosa) essential oils, and their nanoemulsion formulations against six common fish pathogens, Pseudomonas aeruginosa, Vibrio parahaemolyticus, Aeromonas veronii, Vibrio alginolyticus, Yersinia ruckeri, and Lactococcus garvieae. The main components of Mentha spicata subs. tomentosa essential oil (MEO) were piperitone (25.01%), eucalyptol (1,8-cineole) (19.53%), pulegone (14.50%) and, Piperitenone (10.98%). The major components of Origanum onites essential oil (OEO) which were carvacrol (46.17%) and, p-cymene (13.05%) were detected. The antibacterial effects of OEO and MEO and their nanoemulsions were determined by using the agar disc diffusion method. The OEO and its nanoemulsions were extremely effective against the Gram‐negative Aeromonas veronii than the positive control (enrofloxacin). In addition, it was observed that OEO nanoemulsion was more effective than OEO in terms of antibacterial activity. MEO and its nanoemulsions were found to have low activity against fish pathogens, however, there was no activity on Aeromonas veronii and Lactococcus garvieae.

Keywords

Origanum onites, Mentha spicata subs. Tomentosa, Bacterial fish pathogen, Antibacterial activity

Origanum onites ve Mentha spicata subs. tomentosa Uçucu Yağ Nanoemülsiyonlarının Bakteriyel Balık Patojenlerine Karşı in Vitro Antibakteriyel Aktivitesi

Abstract

Bu çalışmanın amacı, iki farklı aromatik bitkinin (Origanum onites, Mentha spicata subs. tomentosa) uçucu yağlarının ve bunların nanoemülsiyon formülasyonlarının altı farklı balık patojeni Pseudomonas aeruginosa, Vibrio parahaemolyticus, Aeoromonas veronii, Yersinia ruckeri, Lactococcus garvieae ve Vibrio alginolyticus'a karşı antimikrobiyal aktivitelerini belirlemektir. Mentha spicata subs. tomentosa uçucu yağının (MEO) ana bileşenleri piperitone, okaliptol (1,8-cineole), pulegone, piperitenone sırasıyla %25.01, %19.53, %14.50 ve %10.98 olarak belirlenmiştir. Origanum onites uçucu yağının (OEO) ana bileşenleri sırasıyla karvakrol, %46.17 ile p-cymene, %13.05 oranında tespit edilmiştir. OEO ve MEO ve bu uçucu yağların nanoemülsiyonlarının antibakteriyel etkileri agar disk difüzyon yöntemi kullanılarak belirlendi. OEO nanoemülsiyonları, Gram-negatif Aeromonas veronii'ye karşı pozitif kontrole (enrofloksasin) göre son derece etkili olduğu tespit edilmiştir. Bunun yanında, antibakteriyel aktivite bakımından OEO nanoemülsiyonlarının, OEO’ya göre daha etkili olduğu görülmüştür. MEO ve nanoemülsiyonlarının balık patojenlerine karşı düşük bir aktiviteye sahipken Aeromonas veronii ve Lactococcus garvieae üzerinde antibakteriyel aktivite göstermemiştir.

Keywords

Origanum onites, Mentha spicata subs. Tomentosa, Bakteriyel balık patojenleri, Antibakteriyel aktivite

References

• Bansemir, A., Blume, M., Schröder, S., & Lindequist, U. (2006). Screening of cultivated seaweeds for antibacterial activity against fish pathogenic bacteria. Aquaculture, 252(1), 7984. https://doi.org/10.1016/j.aquaculture.2005.11.051
• Baratta, M. T., Dorman, H. D., Deans, S. G., Biondi, D. M., & Ruberto, G. (1998). Chemical composition, antimicrobial and antioxidative activity of laurel, sage, rosemary, oregano and coriander essential oils. Journal of Essential Oil Research, 10(6), 618-627. https://doi.org/10.1080/10412905.1998.9700989
• Baydar, H., Sağdiç, O., Özkan, G., & Karadoğan, T. (2004). Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial importance in Turkey. Food Control, 15(3), 169-172. https://doi.org/10.1016/S0956-7135(03)00028-8
• Boukhebti, H., Chaker A. N., Belhadj, H., Sahli, F., Ramdhani, M., Laouer, H., & Harzallah, D. (2011). Chemical composition and antibacterial activity of Mentha pulegium L. and Mentha spicata L. essential oils. Der Pharmacia Lettre, 3(4), 267-275.
• Chang, Y., McLandsborough, L., & McClements, D. J. (2013). Physicochemical properties and antimicrobial efficacy of carvacrol nanoemulsions formed by spontaneous emulsification. Journal of Agricultural and Food Chemistry, 61(37), 8906-8913. https://doi.org/10.1021/jf402147p
• Dastjerdi, L. S., & Mazoji, A. (2015). Comparative chemical composition of the essential oils of Iranian Achillea oxyodonta from different ecological regions. Journal of Applied Pharmaceutical Science, 5(5), 106-109. https://doi.org/10.7324/JAPS.2015.50520
• Dawood, M. A. O., El Basuini, M. F., Zaineldin, A. I., Yilmaz, S., Hasan, M. T., Ahmadifar, E., El Asely, A. M., Abdel-Latif, H. M. R., Alagawany, M., Abu-Elala, N. M., Van Doan, H., & Sewilam, H. (2021). Antiparasitic and antibacterial functionality of essential oils: an alternative approach for sustainable aquaculture. Pathogens, 10(2), 185. https://doi.org/10.3390/pathogens10020185
• Diler, Ö., Özil, Ö., Bayrak, H., Yiğit, N. Ö., Özmen, Ö., Saygın, M., & Aslankoç, R. (2021). Effect of dietary supplementation of sumac fruit powder (Rhus coriaria L.) on growth performance, serum biochemistry, intestinal morphology and antioxidant capacity of rainbow trout (Oncorhynchus mykiss, Walbaum). Animal Feed Science and Technology, 278, 114993. https://doi.org/10.1016/j.anifeedsci.2021.114993
• Gholipourkanani, H., Buller, N., & Lymbery, A. (2019). In vitro antibacterial activity of four nano-encapsulated herbal essential oils against three bacterial fish pathogens. Aquaculture Research, 50(3), 871–875. https://doi.org/10.1111/are.13959
• Hamouda, T., Hayes, M. M., Cao, Z., Tonda, R., Johnson, K., Wright, D. C., Brisker, J., & Baker Jr, J. R. (1999). A novel surfactant nanoemulsion with broad-spectrum sporicidal activity against Bacillus species. Journal of Infectious Diseases, 180(6), 1939-1949. https://doi.org/10.1086/315124
• Hemmila, M. R., Mattar, A., Taddonio, M. A., Arbabi, S., Hamouda, T., Ward, P. A., Wang, S. C., & Baker Jr, J. R. (2010). Topical nanoemulsion therapy reduces bacterial wound infection and inflammation after burn injury. Surgery, 148(3), 499–509. https://doi.org/10.1016/j.surg.2010.01.001
• Hoque, M. S., Jacxsens, L., De Meulenaer, B., & Alam, A. N. (2016). Quantitative risk assessment for formalin treatment in fish preservation: food safety concern in local market of Bangladesh. Procedia Food Science, 6, 151-158. https://doi.org/10.1016/j.profoo.2016.02.037
• Krishnamoorthy, R., Athinarayanan, J., Periasamy, V. S., Adisa, A. R., Al-Shuniaber, M. A., Gassem, M. A., & Alshatwi, A. A. (2018). Antimicrobial activity of nanoemulsion on drug-resistant bacterial pathogens. Microbial Pathogenesis, 120, 85-96. https://doi.org/10.1016/j.micpath.2018.04.035
• Li, T., Raza, S. H. A., Yang, B., Sun, Y., Wang, G., Sun, W., Qian, A., Wang, C., Kang, Y., & Shan, X. (2020). Aeromonas veronii infection in commercial freshwater fish: A potential threat to public health. Animals, 10(4), 608. https://doi.org/10.3390/ani10040608
• Manandhar, S., Luitel, S., & Dahal, R. K. (2019). In Vitro Antimicrobial Activity of Some Medicinal Plants against Human Pathogenic Bacteria. Journal of Tropical Medicine, 1895340. https://doi.org/10.1155/2019/1895340
• Mazandarani, M., Momeji, A., & ZARGHAMI, M. P. (2013). Evaluation of phytochemical and antioxidant activities from different parts of Nasturtium officinale R. Br. İn Mazandaran. Iranian Journal of Plant Physiology, 3(2): 659-664.
• Murray, P. R., Baron, E. J., Pfaller, M. A., Tenover, F. C., & Yolken, R. H. (2011). Manual of Clinical Microbiology (10th edn). ASM Press.
• Okmen,, G., Ugur, A., Sarac, N., & Arslan, T. (2012). In vivo and in vitro antibacterial activities of some essential oils of lamiaceae species on Aeromonas salmonicida isolates from cultured rainbow trout, Oncorhynchus mykiss. Journal of Animal Veterinary Advances, 11, 2762– 2768. https://doi.org/10.3923/javaa.2012.2762.2768
• Ozkan, G., Baydar, H., & Erbas, S. (2010). The influence of harvest time on essential oil composition, phenolic constituents, and antioxidant properties of Turkish oregano (Origanum onites L.). Journal of the Science of Food and Agriculture, 90(2), 205–209. https://doi.org/10.1002/jsfa.3788
• Pablos, M., Huys, G., Cnockaert, M., Rodríguez-Calleja, J. M., Otero, A., Santos, J. A., & García-López, M. L. (2011). Identification and epidemiological relationships of Aeromonas isolates from patients with diarrhea, drinking water and foods. International Journal of Food Microbiology, 147(3), 203-210. https://doi.org/10.1016/j.ijfoodmicro.2011.04.006
• Proestos, C., Chorianopoulos, N., Nychas, G. J., & Komaitis, M. (2005). RP-HPLC analysis of the phenolic compounds of plant extracts. Investigation of their antioxidant capacity and antimicrobial activity. Journal of Agricultural and Food Chemistry, 53(4), 1190-1195. https://doi.org/10.1021/jf040083t
• Reverter, M., Bontemps, N., Lecchini, D., Banaigs, B., & Sasal, P. (2014). Use of plant extracts in fish aquaculture as an alternative to chemotherapy: current status and future perspectives. Aquaculture 433, 50-61. https://doi.org/10.1016/j.aquaculture.2014.05.048
• Sarac, N., & Ugur, A. (2008). Antimicrobial activities of the essential oils of Origanum onites L., Origanum vulgare L. subspecies hirtum (Link) Ietswaart, Satureja thymbra L., and Thymus cilicicus Boiss. & Bal. growing wild in Turkey. Journal of Medicinal Food, 11(3), 568-573. https://doi.org/10.1089/jmf.2007.0520
• Sevindik, E., Abacı, Z. T., Yamaner, C., & Ayvaz, M. (2016). Determination of the chemical composition and antimicrobial activity of the essential oils of Teucrium polium and Achillea millefolium grown under North Anatolian ecological conditions. Biotechnology & Biotechnological Equipment, 30(2), 375-380. https://doi.org/10.1080/13102818.2015.1131626
• Sevindik, M., Akgul, H., Pehlivan, M., & Selamoglu, Z. (2017). Determination of therapeutic potential of Mentha longifolia ssp. longifolia. Fresenius Environmental Bulletin, 26(7), 4757-4763.
• Shan, B., Cai, Y. Z., Brooks, J. D., & Corke, H. (2011). Potential application of spice and herb extracts as natural preservatives in cheese. Journal of Medicinal Food, 14(3), 284-290. https://doi.org/10.1089/jmf.2010.0009
• Starliper, C. E., Ketola, H. G., Noyes, A. D., Schill, W. B., Henson, F. G., Chalupnicki, M. A., & Dittman, D. E. (2015). An investigation of the bactericidal activity of selected essential oils to Aeromonas spp. Journal of Advanced Research, 6(1), 89–97. https://doi.org/10.1016/j.jare.2013.12.007
• Sarer, E., Toprak, S. Y., Otlu, B., & Durmaz, R. (2011). Composition and antimicrobial activity of the essential oil from Mentha spicata L. subsp. spicata. Journal of Essential Oil Research, 23(1), 105-108. https://doi.org/10.15835/nbha3723188
• Toncer, O., Karaman, S., Kızıl, S., & Diraz, E. (2009). Changes in essential oil composition of oregano (Origanum onites L.) due to diurnal variations at different development stages. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 37(2), 177-181.