Çardak Lagünü'nde (Çanakkale Boğazı) Bulunan Portunid Yengeç, Carcinus aestuarii Nardo, 1847'nin Hemolenfinde Çevresel Değişkenlerin ve Morfometrinin Hemosit Yapısına Etkileri

Year 2022, Volume: 18 Issue: 4, 505 - 512, 01.12.2022

Seçil Acar A. Suat Ateş

https://doi.org/10.22392/actaquatr.1150615

Abstract

Bu çalışma, Carcinus aestuarii'de hemosit sayısı ile hemosit tipleri arasındaki ilişkiyi çevresel değişkenler ve morfometrik ölçümlerle belirlemeyi amaçlamaktadır. Çardak Lagünü'nde Haziran 2018, Ekim 2018, Şubat 2019 ve Mayıs 2019'da mevsimsel olarak toplam 240 yengeç toplanmıştır. Ortalama kabuk uzunluğu (CL) 44.66±0.44 mm, ortalama kabuk genişliği (CW) 37.71±0.38 mm ve ortalama ağırlık 28.49±0.88 g’ idi. Yengeç örnekleri buz üzerinde 10 dakika süreyle anesteziye tabi tutuldu ve 1 mL'lik plastik bir şırınga kullanılarak her bir yengecin bacaklarından 500 uL hemolenf toplandı. Örneklemeden sonra hemolenf örnekleri bir Thoma lamına uygulanan antikoagülan ile karıştırıldı ve X40 büyütmede mikroskop altında incelendi. Toplam hemosit sayısı (THC) 13.91±8.08x106 (hücre/mm3) olarak hesaplandı. Diferansiyel hemosit sayısı (DHC) hiyalinosit için % 53.25±0.6, yarı granülosit için % 26.15±0.23 ve granülosit için % 20.6±0.06 ve 3 farklı hücre tipi gözlendi. En baskın hücre tipi hyalinosit hücreleridir.

Keywords

Toplam hemosit sayısı, Hyaline, Granuler, Fizikokimyasal değişkenler, Akdeniz yeşil yengeci

The Effects of Environmental Variables and Morphometry on Hemocyte Structure in the Hemolymph of Portunid Crab, Carcinus aestuarii Nardo, 1847 in Çardak Lagoon (Çanakkale Strait)

Abstract

This study aims to determine the relationship between hemocyte count and hemocyte types with environmental variables and morphometric measurements in Carcinus aestuarii. A total of 240 crabs were seasonally collected in Çardak Lagoon June 2018, October 2018, February 2019, and May 2019. The average carapace length (CL) was 44.66±0.44 mm, the mean carapace width (CW) was 37.71±0.38 mm and the mean weight was 28.49±0.88 g. Crab specimens were anesthetized on ice for 10 min, and 500 µL of hemolymph was collected from the legs of each crab using a 1-mL plastic syringe. After sampling, hemolymph samples were mixed with the anticoagulant, which was applied to a Thoma slide and examined under a microscope at X40 magnification. The total hemocyte count (THC) was calculated as 13.91±8.08x106 (cells/mm3). Differential hemocyte count (DHC) 53.25±0.6% for hyalinocyte, 26.15±0.23% for semi-granulocyte, and 20.6±0.06% for granulocyte and 3 different cell types were observed. The most dominant cell type is hyalinocyte cells.

Keywords

Total hemocyte counts, Hyaline, Granular, Physicochemical variables, Mediterranean Green Crab

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