A New Strategy: Antibiotic Circumstances for Rational Drug use Against Clinical Aeromonas hydrophila And Bacterial Properties of This Bacteria on Different Agars

Year 2024, Volume: 20 Issue: 4, 312 - 325, 01.12.2024

Nurdan Filik , Fethi Filik , Ayşegül Kubilay

https://doi.org/10.22392/actaquatr.1439588

Abstract

Antibiotic resistance of A. hydrophila was once again observed on different agars. Random antibiotics drug treatment of diseases causes development resistance. Thus, we have faced post-antibiotic era in which our ability to challenge bacteria has diminished and the need for new strategies to deal with disease has increased. A. hydrophila ATCC reference strain, which causes the fatal Motil Aeromonas Septicemia (MAS) Disease in fish, was used in the study Colony structure of A. hydrophila formed on MacConkey (MAC), Aeromonas Isolation Base Agar (AIBA), Congo Red Agar (CRA) and Blood Agar (BA) were examined. The antibiotic susceptibility was determined by using the Kirby-Bauer method Mueller–Hinton Agar, MAC, AIBA and CRA agar plates. A. hydrophila was found sensitive to ciprofloxacin, enrofloxacin, gentamicin, sulphamethoxazole/trimethoprim, and resistant to penicillin G and oxacillin. The important difference was obtained as resistant to enrofloxacin on MAC. Multiple antibiotic resistance index (MARI) of A. hydrophila was determined as 0.33 in MHA and 0.5 in MAC. This difference was due to the antibiotic enroflaxin, and its appearance in this study, where a different new approach was tried for the first time, also added originality to the subject. This status may be related to an acquired plasmid. It is important to try to see resistance of A. hydrophila by using different agars while innovations such as the AntibiogramJ program are being tried to be added to antibiotic literature.

Keywords

Motil aeromonas septicemia (MAS) disease, Antibiotic resistance, Aeromonas Isolation Base Agar (AIBA), Congo Red Agar (CRA)

Yeni Bir Strateji: Klinik Aeromonas hydrophila'ya Karşı Akılcı İlaç Kullanımı İçin Antibiyotik Durumları ve Bu Bakterilerin Farklı Agarlar Üzerindeki Bakteriyel Özellikleri

Abstract

Rastgele antibiyotiklerle hastalıkların ilaçla tedavisi direnç gelişimine neden olmaktadır. Böylece bakterilerle mücadele yeteneğimizin azaldığı ve hastalıklarla mücadelede yeni stratejilere olan ihtiyacın arttığı antibiyotik sonrası dönemle karşı karşıya kalmış durumdayız. Araştırma makalesinde, balıklarda ölümcül Motil Aeromonas Septicemia (MAS) Hastalığına neden olan, A. hydrophila ATCC referans suşu kullanılmıştır. MacConkey (MAC), Aeromonas Isolation Base Agar (AIBA), Congo Red Agar (CRA) ve Blood Agar (BA) üzerinde oluşan A. hydrophila kolonileri incelenmiştir. Antibiyotik duyarlılığı Kirby-Bauer yöntemi Mueller-Hinton Agar, MAC, AIBA ve CRA agar plakları kullanılarak belirlendi. A. hydrophila siprofloksasin, enrofloksasin, gentamisin, sülfametoksazol/trimetoprim'e duyarlı bulunurkan penisilin G ve oksasiline dirençli bulunmuştur. Önemli bir fark olarak A. hydrophila'nın MAC'ta enrofloksasine dirençli olduğu görülmüştür. A. hydrophila'nın çoklu antibiyotik direnç indeksi (MARI) MHA'da 0,33, MAC'da 0,5 olarak belirlendi. Bu farklılık enrofloksasin antibiyotiğinde tespit edilmiş ve ilk kez farklı bir yaklaşımın denendiği bu çalışmada bu durumun ortaya çıkması konuya özgünlük de katmıştır. Bu durumun edinilmiş bir plazmid ile ilgili olabileceği kanaatindeyiz. AntibiogramJ programı gibi yenilikler antibiyotik literatürüne kazandırılmaya çalışılırken, farklı agarlar kullanılarak A. hydrophila'nın direncinin görülmeye çalışılması önemlidir. A. hydrophila'nın antibiyotik direnci farklı agarlarda bir kez daha gözlendi.

Keywords

Motil aeromonas septisemi (MAS) hastalığı, Antibiyotik direnci, Aeromonas Isolation Base Agar (AIBA), Congo Red Agar (CRA)

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