Molecular Epidemiology and Risk Factors Assessment of Anaplasma spp. on Dairy Cattle in Southwest of Iran

Year 2019, Volume: 45 Issue: 1, 30 - 36, 01.01.2019

Vahid Noaman Morteza Moradı

Abstract

DOI:
10.26650/actavet.2019.18014

The present study was carried out to
determine Anaplasma species and potential risk factors associated with
molecular prevalence of Anaplasma spp. among dairy cattle in southwest of Iran.
A total of 88 samples out of 200 generated an expected amplicon of 866 bp from
Anaplasma marginale msp4 gene. Six samples that were identified as A. marginale
gave also positive results for A. phagocytophilum 16S rRNA gene with specific
nested polymerase chain reaction (nPCR). The multivariate analysis of risk
factors revealed that the cattle of mountain regions were significantly
(p=0.0001) at higher risk as compared to the plain regions. Cattle <1 year
age and the latitude 32-33°C were significantly at lower risk (p<0.01). The
cattle with low milk yield were significantly (p=0.002) at lower risk. Low
hygienic farms were significantly (p=0.011) at higher risk as compared to good
and normal hygienic farms. Distance from other farms (<1Km) was another
important risk factor which showed significant association with the occurrence
of Anaplasma infection (p=0.021). The results of this study can be used in
strategic planning for prevention and control of bovine anaplasmosis in dairy
cattle in the southwest of Iran.

Keywords

Anaplasma, cattle, Iran, molecular epidemiology, risk factors

References

• Amorim, L.S., Wenceslau, A.A., Carvalho, F.S., Carneiro, P.L., Albuquerque, G.R., 2014. Bovine babesiosis and anaplasmosis complex: diagnosis and evaluation of the risk factors from Bahia, Brazil. Revista Brasileira de Parasitologia Veterinaria 23, 328-336. Atif, F.A., 2015. Anaplasma marginale and Anaplasma phagocytophilum: Rickettsiales pathogens of veterinary and public health significance. Parasitology Research 114, 3941-3957. Atif, F.A., Khan, M.S., Muhammad, F., Ahmad, B., 2013. Sero-epidemiological study of Anaplasma marginale among cattle, Journal of Animal and Plant Sciences 23, 740-4. Aubry, P., Geale, D.W., 2011. A review of bovine anaplasmosis. Transboundary and Emerging Diseases 58, 1-30. Bakken, J.S., Dumler, J.S., 2015. Human granulocytic anaplasmosis. Infectious Disease Clinics of North America 29, 341-355. Barlough, J.E., Madigan, J.E., DeRock, E., Bigornia, L., 1996. Nested polymerase chain reaction for detection of Ehrlichia equi genomic DNA in horses and ticks (Ixodes pacificus). Veterinary Parasitology 63, 319-329. Costa, V.M., Ribeiro, M.F., Duarte, A.L., Mangueira, J.M., Pessoa, A.F., Azevedo, S.S., Barros, A.T., Riet-Correa, F., Labruna, M.B., 2013. Seroprevalence and risk factors for cattle anaplasmosis, babesiosis, and trypanosomiasis in a Brazilian semiarid region. Revista Brasileira de Parasitologia Veterinaria 22, 207-213. Dantas-Torres, F., 2015. Climate change, biodiversity, ticks and tick-borne diseases: The butterfly effect. International Journal for Parasitology: Parasites and Wildlife 4, 452-461. da Silva, J.B., da Fonseca, A.H., 2013. Analysis of the risk factors related to the immune humoral anti-Anaplasma marginale in dairy cattle. Semina: Ciencias Agrarias 34, 777-784. da Silva, J.B., da Fonseca, A.H., 2014. Risk factors for anaplasmosis in dairy cows during the peripartum. Tropical Animal Health and Production 46, 461-465. da Silva, J.B., de Santana Castro, G.N., Fonseca, A.H., 2014. Longitudinal study of risk factors for anaplasmosis and transplacental transmission in herd cattle. Semina: Ciencias Agrarias 35, 2491-2500. de la Fuente, J., Van Den Bussche, R.A., Garcia-Garcia, J.C., Rodriguez, S.D., Garcia, M.A., Guglielmone, A.A., Mangold, A.J., Friche Passos, L.M., Barbosa Ribeiro, M.F., Blouin, E.F. Kocan, K.M., 2002. Phylogeography of New World isolates of Anaplasma marginale based on major surface protein sequences. Veterinary Microbiology 88, 275-285. Estrada-Peña, A., Salman, M., 2013. Current limitations in the control and spread of ticks that affect livestock: a review. Agriculture 3, 221-235. Inokuma, H., Terada, Y., Kamio, T., Raoult, D., Brouqui, P., 2001. Analysis of the 16S rRNA gene sequence of Anaplasma centrale and its phylogenetic relatedness to other ehrlichiae. Clinical and Diagnostic Laboratory Immunology 8, 241-244. Kawahara, M., Rikihisa, Y., Lin, Q., Isogai, E., Tahara, K., Itagaki, A., Hiramitsu, Y., Tajima, T., 2006. Novel genetic variants of Anaplasma phagocytophilum, Anaplasma bovis, Anaplasma centrale, and a novel Ehrlichia sp. In wild deer and ticks on two major islands in Japan. Applied and Environmental Microbiology 72, 1102-1109. Kispotta, S., Islam, M.F., Hoque, M.F., Rahman, M.S., Borman, A., Haque, M.A., Rahman, M.R., 2017. Study of prevalence and associated risk factors of anaplasmosis and theileriasis in cattle. Asian Journal of Medical and Biological Research 2, 567-576. Kocan, K.M., de la Fuente, J., Blouin, E.F., Coetzee, J.F., Ewing, S.A., 2010. The natural history of Anaplasma marginale. Veterinary Parasitology 167, 95-107. Kocan, K.M., de la Fuente, J., Cabezas-Cruz, A., 2015. The genus Anaplasma: new challenges after reclassification. Revue Scientifique et Technique (International Office of Epizootics) 34, 577-586. Milner, J.M., van Beest, F.M., 2013. Ecological correlates of a tick-borne disease, Anaplasma phagocytophilum, in moose in Southern Norway. European Journal of Wildlife Research 59, 399-406. Noaman, V., 2012. Identification of hard ticks collected from sheep naturally infected with Anaplasma ovis in Isfahan province, central Iran. Comparative Clinical. Pathology 21, 367-369. Noaman, V., 2013a. Discrimination between Anaplasma marginale and Anaplasma ovis by PCR-RFLP. World Applied Sciences Journal 21, 190-195. Noaman, V., 2013b. Report of Anaplasma centrale (Amori strain) in cattle in Iran [Persian]. Pajouhesh-va-Sazandegi Veterinary Journal 98, 26-29. Noaman, V., Abdigoudarzi, M., Nabinejad, A.R., 2017. Abundance, diversity and seasonal dynamics of hard ticks infesting cattle in Isfahan province, central Iran. Archives of Razi Institute 72, 15-21. Noaman, V., Bastani, D., 2016. Molecular study on infection rates of Anaplasma ovis and Anaplasma marginale in sheep and cattle in West-Azerbaijan province, Iran. Veterinary Research Forum 7, 163-167. Noaman, V., Shayan, P., 2009. Molecular detection of Anaplasma phagocytophilum in carrier cattle of Iran-first documented report. Iranian Journal of Microbiology 1, 37-42. Noaman, V., Shayan, P., 2010a. Comparison of microscopic methods and PCR-RFLP for detection of Anaplasma marginale in carrier cattle. Iranian Journal of Microbiology 2, 89-94. Noaman, V., Shayan, P., 2010b. Molecular detection of Anaplasma bovis in cattle from central part of Iran. Veterinary Research Forum 1, 117-122. Noaman, V., Shayan, P., Amininia, N., 2009. Molecular diagnostic of Anaplasma marginale in carrier cattle. Iranian Journal of Parasitology 4, 31-38. Noaman, V., Nabinejad, A., Shahmoradi, A., Esmaeilkhanian, S., 2016. Molecular detection of bovine leukocytic Anaplasma species in Isfahan, Iran. Research in Molecular Medicine Journal 4, 47-51. Quiroz-Castaneda, R.E., Amaro-Estrada, I., Rodriguez-Camarillo, S.D., 2016. Anaplasma marginale: Diversity, virulence, and vaccine landscape through a genomics approach. BioMed Research International 2016, 1-18. Rahman, A.S.M.S., Sumon, S.M.M.R., Khan, M.A.H.N.A., Islam, M.T., 2015. Current status of subclinical form of babesiosis and anaplasmosis in cattle at Rangpur district in Bangladesh. Progressive Agriculture 26, 51-59. Sajid, M.S., Siddique, R.M., Khan, S.A., Iqbal, Z., Khan, M.N., 2014. Prevalence and risk factors of anaplasmosis in cattle and buffalo populations of district Khanewal, Punjab, Pakistan. Global Veterinaria 12, 146-153. Sharma, A., Singla, L.D., Kaur, P., Bal, M.S., 2015. PCR and ELISA vis-à-vis microscopy for detection of bovine anaplasmosis: a study on associated risk of an upcoming problem in North India. The Scientific World Journal, 1-8. Walker, A.R., 2014. Ticks and associated diseases: a retrospective review. Medical and Veterinary Entomology 28, 1-5. Weisburg, W.G., Barns, S.M., Pelletier, D.A., 1991. 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology 173, 697-703. Zarasvandi, A., Carranza, E.J.M., Moore, F., Rastmanesh, F., 2011. Spatio-temporal occurrences and mineralogical-geochemical characteristics of airborne dusts in Khuzestan province (Southwestern Iran). Journal of Geochemical Exploration 111, 138-151.