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Assessment of cutaneous and serum oxidative stress changes in dogs infested with Sarcoptes scabiei var canis

Year 2019, Volume , Issue , 0 - 0, 01.01.2019

Abstract

The evaluation of oxidative and anti-oxidative activities in skin and serum of scabietic dogs was studied. 20 healthy and 5 scabietic dogs were used. Healthy dogs were grouped as group A (Control; 10 dogs) and group B (10 dogs). Group B dogs were housed with the 5 scabietic dogs. Hydrogen peroxide (H2O2), Glutathione peroxidase (GPX), Glutathione-S-transferase (GST), Superoxide dismutase (SOD), Reduced glutathione (GSH), and Total protein (TP) activities were weighed in skin and serum of healthy and scabietic dogs. Malonyldialdehyde (MDA) amount was measured in skin while Myeloperoxidase (MPO) amount was measured in serum of both healthy and scabietic dogs. Increase and decrease in free radical generation (H2O2) was noted with the use of serum and skin samples while GPX and SOD amounts in skin and serum was significantly higher in scabietic dogs. GST and GSH values were significantly higher in skin sample whereas GSH amount was not altered in serum. Total protein values decreased significantly in serum but decreased insignificantly in skin of infested dogs. MDA (skin) and MPO (serum) amounts were elevated in scabietic dogs. Results showed that skin could be alternatives or complementary care samples in evaluation of oxidant, antioxidant balance, prognosis and monitoring of scabies therapy. Cite this article as: Nwufoh, O.C., Sadiq, N.A., Emikpe, B.O., Omobowale, T., 2020. Assessment of cutaneous and serum oxidative stress changes in dogs infested with Sarcoptes scabiei var canis. Acta Vet Eurasia, DOI: 10.5152/actavet.2020.19010

References

  • 1. Allaam, M.A., Allam, T.S., Elkhatam, A.O. 2014. Biochemical and Circulating Oxidative Stress Biomarkers in Egyptian Buffaloes (Bubalus bubalis) Infested by Sarcoptic Mange. Global Veterinaria 13: 656-661. DOI:10.5829/idosi.gv.2014.13.04.8680. 2. Beigh, S.A., Soodan, J.S., Singh, R., Khan, A.M., Dar, M.A. 2013. Evaluation of trace elements, oxidant/antioksidant status, vitamin C and β-carotene in dogs with dermatophytosis. Mycoses: 57: 358-365. 3. Bernabucci, U., Ronchi, B., Lacetera, N., Nardone, A. 2005. Influence of body condition score on relationships between metabolic status and oxidative stress in periparturient dairy cows. Journal of Dairy Science, 88: 2017–2026. 4. Beutler, E., Duron, O., Kelly, B.M. 1963. Improved method for the determination of blood glutathione. Journal of Laboratory Clinical Medicine, 61:882-888. 5. Bickers, D.R., Athar, M. 2006. Oxidative stress in the pathogenesis of skin disease. Journal of Investigative Dermatology, 126: 2565-2575. 6. Camkerten, I., Sahin, T., Borazan, G., Gokcen, A., Erel, O., Das, A. 2009. Evaluation of blood oxidant/antioxidant balance in dogs with sarcoptic mange. Veterinary Parasitology, 161: 106- 109. 7. Baumann, C.W., Kwak, D., Liu, H.M., Thompson, L.V. 2016. Age-induced oxidative stress: how does it influence skeletal muscle quantity and quality? Journal of Applied Physiology, 121: 1047- 1052. 8. Cross, C.E., van der Vliet, A., Louie, S., Thiele, J.J., Halliwell, B. 1998. Oxidative stress and antioxidants at biosurfaces: plants, skin and respiratory tract surfaces. Environmental Health Perspective, 106: 1241–1251. 9. Dimri, U., Sharma, M.C., Swarup, D., Ranjan, R., Kataria, M. 2008b. Alterations in hepatic lipid peroxides and antioxidant profile in Indian water buffaloes suffering from sarcoptic mange. Research Veterinary Science, 85: 101–105. 10. Dimri, U., Ranjan, R., Kumar, N., Sharma, M.C., Swarup, D., Sharma, B., Kataria, M. 2008a. Changes in oxidative stress indices, zinc and copper concentrations in blood in canine demodicosis. Veterinary Parasitology, 154:98–102. 11. Dimri, U., Sharma, M.C., Yamdagni, A., Ranjan, R., Zama, M.M.S. 2010. Psoroptic mange infestation increases oxidative stress and decreases antioxidant status in sheep. Veterinary Parasitology, 168: 318–322 12. Fain, A. 1968. Etude de la variabilité de Sarcoptes scabiei avec une révision des Sarcoptidae. Acta Zoologica et Pathologica Antverpiensia, 47: 1–196. 13. Fang, Y.Z., Yang, S., Wu, G. 2002. Free radicals, antioxidants, and nutrition. Nutrition, 18: 872– 879. 14. Gornall, A.G., Bardawill, C.J., David, M.M. 1949. Determination of serum proteins by means of the biuret reaction. Journal of Biology Chemistry, 177: 751-766. 15. Habig, W.H., Pabst, M.J., Jacoby, W.B. 1974. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry, 249: 7130–7139. 16. Jollow, D.J., Mitchell, J.R., Zampaglione, N., Gillette, J.R. 1974. Bromobenzene-induced liver necrosis. Protective role of glutathione and evidence for 3,4-bromobenzene oxide as the hepatotoxic metabolite. Pharmacology, 11: 151-169 17. Knight, J.A. 2000. Review: free radicals, antioxidants, and the immune system. Annals of Clinical Laboratory Science, 30: 145–158. 18. Kocyigit, A., Keles, H., Selek, S., Guzel, S., Celik, H., Erel, O. 2005. Increased DNA damage and oxidative stress in patients with cutaneous leishmaniasis. Mutat Research, 585: 71–78. 19. Rinnerthaler, M., Bischof, J., Streubel, M.K., Trost, A., Richter, K. 2015. Oxidative stress in aging human skin. Biomolecules, 5: 545-589. 20. Nwufoh, O.C., Sadiq, N.A., Emikpe, B.O. 2018. Establishment of infestivity model for Sarcoptes scabiei var canis in Nigerian dogs. Journal of Parasitic Diseases, 42: 519-526. 21. Nwufoh, O.C., Sadiq, N.A., Emikpe, B.O. 2019. Clinicopathological and mineral changes associated with dogs infested with Sarcoptes scabiei var. canis. Comparative Clinical Pathology, doi:10.1007/s00580-019-02919-w 22. Omobowale, T.O., Oyagbemi, A.A., Oyewunmi, O.A., Adejumobi, A.O. 2015. Chemopreventive effect of methanolic extract of Azadirachta indica on experimental Trypanosoma brucei induced oxidative stress in dogs. Pharmacology Research, 7: 249-258. 23. Saleh, M.A., Mahran, O.M., Bassam Al-Salahy, M. 2010. Circulating oxidative stress status in dromedary camels infested with sarcoptic mange. Veterinary Research Communications, 35, 35–45. doi:10.1007/s11259-010-9450-x 24. Singh, S.K., Dimri, U., Sharma, M.C., Swarup, D., Sharma, B., 2011. Determination of oxidative status and apoptosis in peripheral blood of dogs with sarcoptic mange. Journal of Veterinary Parasitology, 178: 330-338. 25. Smith, N.C., Bryant, C., 1989. Free radical generation during primary infections with Nippostrongylus brasiliensis. Parasite immunology, 11: 147-160. 26. Soulsby, E.J.L. 1982. Helminths, Arthropods and Protozoa of Domesticated Animals. 7 th Edition, Baillieri Tindal, London. 27. Trouba, K.J., Hamadeh, H.K., Amin, R.P., Germolec, D.R. 2002. Oxidative stress and its role in skin disease. Antioxidants & Redox Signaling, 4: 665-673. 28. Varshney, R., Kale, R.K., 1990. Effects of calmodulin antagonists on radiation-induced lipid peroxidation in microsomes. International Journal of Radiation Biology, 58: 733-743 29. Walton, S.F., 2010. The immunology of susceptibility and resistance to scabies. Parasite Immunology, 32: 532–540. 30. Walton, S.F., Currie, B.J., 2007. Problems in diagnosing scabies, a global disease in human and animal populations. Clinical Microbiology Reviews, 20: 268-279. 31. Wolff, S.P. 1994. Ferrous ion oxidation in the presence of ferric ion indicator xylenol orange for measurement of hydroperoxides. Methods in Enzymology, 233: 182-189. 32. Xia, Y., Zweier, J.L., 1997. Measurement of myeloperoxidase in leukocyte-containing tissues. Analytical biochemistry, 245:93-96

Year 2019, Volume , Issue , 0 - 0, 01.01.2019

Abstract

References

  • 1. Allaam, M.A., Allam, T.S., Elkhatam, A.O. 2014. Biochemical and Circulating Oxidative Stress Biomarkers in Egyptian Buffaloes (Bubalus bubalis) Infested by Sarcoptic Mange. Global Veterinaria 13: 656-661. DOI:10.5829/idosi.gv.2014.13.04.8680. 2. Beigh, S.A., Soodan, J.S., Singh, R., Khan, A.M., Dar, M.A. 2013. Evaluation of trace elements, oxidant/antioksidant status, vitamin C and β-carotene in dogs with dermatophytosis. Mycoses: 57: 358-365. 3. Bernabucci, U., Ronchi, B., Lacetera, N., Nardone, A. 2005. Influence of body condition score on relationships between metabolic status and oxidative stress in periparturient dairy cows. Journal of Dairy Science, 88: 2017–2026. 4. Beutler, E., Duron, O., Kelly, B.M. 1963. Improved method for the determination of blood glutathione. Journal of Laboratory Clinical Medicine, 61:882-888. 5. Bickers, D.R., Athar, M. 2006. Oxidative stress in the pathogenesis of skin disease. Journal of Investigative Dermatology, 126: 2565-2575. 6. Camkerten, I., Sahin, T., Borazan, G., Gokcen, A., Erel, O., Das, A. 2009. Evaluation of blood oxidant/antioxidant balance in dogs with sarcoptic mange. Veterinary Parasitology, 161: 106- 109. 7. Baumann, C.W., Kwak, D., Liu, H.M., Thompson, L.V. 2016. Age-induced oxidative stress: how does it influence skeletal muscle quantity and quality? Journal of Applied Physiology, 121: 1047- 1052. 8. Cross, C.E., van der Vliet, A., Louie, S., Thiele, J.J., Halliwell, B. 1998. Oxidative stress and antioxidants at biosurfaces: plants, skin and respiratory tract surfaces. Environmental Health Perspective, 106: 1241–1251. 9. Dimri, U., Sharma, M.C., Swarup, D., Ranjan, R., Kataria, M. 2008b. Alterations in hepatic lipid peroxides and antioxidant profile in Indian water buffaloes suffering from sarcoptic mange. Research Veterinary Science, 85: 101–105. 10. Dimri, U., Ranjan, R., Kumar, N., Sharma, M.C., Swarup, D., Sharma, B., Kataria, M. 2008a. Changes in oxidative stress indices, zinc and copper concentrations in blood in canine demodicosis. Veterinary Parasitology, 154:98–102. 11. Dimri, U., Sharma, M.C., Yamdagni, A., Ranjan, R., Zama, M.M.S. 2010. Psoroptic mange infestation increases oxidative stress and decreases antioxidant status in sheep. Veterinary Parasitology, 168: 318–322 12. Fain, A. 1968. Etude de la variabilité de Sarcoptes scabiei avec une révision des Sarcoptidae. Acta Zoologica et Pathologica Antverpiensia, 47: 1–196. 13. Fang, Y.Z., Yang, S., Wu, G. 2002. Free radicals, antioxidants, and nutrition. Nutrition, 18: 872– 879. 14. Gornall, A.G., Bardawill, C.J., David, M.M. 1949. Determination of serum proteins by means of the biuret reaction. Journal of Biology Chemistry, 177: 751-766. 15. Habig, W.H., Pabst, M.J., Jacoby, W.B. 1974. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry, 249: 7130–7139. 16. Jollow, D.J., Mitchell, J.R., Zampaglione, N., Gillette, J.R. 1974. Bromobenzene-induced liver necrosis. Protective role of glutathione and evidence for 3,4-bromobenzene oxide as the hepatotoxic metabolite. Pharmacology, 11: 151-169 17. Knight, J.A. 2000. Review: free radicals, antioxidants, and the immune system. Annals of Clinical Laboratory Science, 30: 145–158. 18. Kocyigit, A., Keles, H., Selek, S., Guzel, S., Celik, H., Erel, O. 2005. Increased DNA damage and oxidative stress in patients with cutaneous leishmaniasis. Mutat Research, 585: 71–78. 19. Rinnerthaler, M., Bischof, J., Streubel, M.K., Trost, A., Richter, K. 2015. Oxidative stress in aging human skin. Biomolecules, 5: 545-589. 20. Nwufoh, O.C., Sadiq, N.A., Emikpe, B.O. 2018. Establishment of infestivity model for Sarcoptes scabiei var canis in Nigerian dogs. Journal of Parasitic Diseases, 42: 519-526. 21. Nwufoh, O.C., Sadiq, N.A., Emikpe, B.O. 2019. Clinicopathological and mineral changes associated with dogs infested with Sarcoptes scabiei var. canis. Comparative Clinical Pathology, doi:10.1007/s00580-019-02919-w 22. Omobowale, T.O., Oyagbemi, A.A., Oyewunmi, O.A., Adejumobi, A.O. 2015. Chemopreventive effect of methanolic extract of Azadirachta indica on experimental Trypanosoma brucei induced oxidative stress in dogs. Pharmacology Research, 7: 249-258. 23. Saleh, M.A., Mahran, O.M., Bassam Al-Salahy, M. 2010. Circulating oxidative stress status in dromedary camels infested with sarcoptic mange. Veterinary Research Communications, 35, 35–45. doi:10.1007/s11259-010-9450-x 24. Singh, S.K., Dimri, U., Sharma, M.C., Swarup, D., Sharma, B., 2011. Determination of oxidative status and apoptosis in peripheral blood of dogs with sarcoptic mange. Journal of Veterinary Parasitology, 178: 330-338. 25. Smith, N.C., Bryant, C., 1989. Free radical generation during primary infections with Nippostrongylus brasiliensis. Parasite immunology, 11: 147-160. 26. Soulsby, E.J.L. 1982. Helminths, Arthropods and Protozoa of Domesticated Animals. 7 th Edition, Baillieri Tindal, London. 27. Trouba, K.J., Hamadeh, H.K., Amin, R.P., Germolec, D.R. 2002. Oxidative stress and its role in skin disease. Antioxidants & Redox Signaling, 4: 665-673. 28. Varshney, R., Kale, R.K., 1990. Effects of calmodulin antagonists on radiation-induced lipid peroxidation in microsomes. International Journal of Radiation Biology, 58: 733-743 29. Walton, S.F., 2010. The immunology of susceptibility and resistance to scabies. Parasite Immunology, 32: 532–540. 30. Walton, S.F., Currie, B.J., 2007. Problems in diagnosing scabies, a global disease in human and animal populations. Clinical Microbiology Reviews, 20: 268-279. 31. Wolff, S.P. 1994. Ferrous ion oxidation in the presence of ferric ion indicator xylenol orange for measurement of hydroperoxides. Methods in Enzymology, 233: 182-189. 32. Xia, Y., Zweier, J.L., 1997. Measurement of myeloperoxidase in leukocyte-containing tissues. Analytical biochemistry, 245:93-96

Details

Primary Language English
Subjects Veterinary
Journal Section Original Article
Authors

Onyeka Chidiebele NWUFOH This is me
Department of Veterinary Parasitology, University of Ibadan, Ibadan, Nigeria
Andorra


Nurudeen Ayinde SADİQ This is me
Department of Veterinary Parasitology, University of Ibadan, Ibadan, Nigeria
Andorra


Benjamin Obukowho EMİKPE This is me
Department of Veterinary Pathology, University of Ibadan, Ibadan, Nigeria
Andorra


Tayo OMOBOWALE This is me
Department of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria
Andorra

Publication Date January 1, 2019
Published in Issue Year 2019, Volume , Issue

Cite

Bibtex @research article { actavet674615, journal = {Acta Veterinaria Eurasia}, issn = {2618-639X}, eissn = {2619-905X}, address = {}, publisher = {İstanbul University-Cerrahpasa}, year = {2019}, pages = {0 - 0}, title = {Assessment of cutaneous and serum oxidative stress changes in dogs infested with Sarcoptes scabiei var canis}, key = {cite}, author = {Nwufoh, Onyeka Chidiebele and Sadiq, Nurudeen Ayinde and Emikpe, Benjamin Obukowho and Omobowale, Tayo} }
APA Nwufoh, O. C. , Sadiq, N. A. , Emikpe, B. O. & Omobowale, T. (2019). Assessment of cutaneous and serum oxidative stress changes in dogs infested with Sarcoptes scabiei var canis . Acta Veterinaria Eurasia , , 0-0 . Retrieved from https://dergipark.org.tr/en/pub/actavet/issue/48090/674615
MLA Nwufoh, O. C. , Sadiq, N. A. , Emikpe, B. O. , Omobowale, T. "Assessment of cutaneous and serum oxidative stress changes in dogs infested with Sarcoptes scabiei var canis" . Acta Veterinaria Eurasia (2019 ): 0-0 <https://dergipark.org.tr/en/pub/actavet/issue/48090/674615>
Chicago Nwufoh, O. C. , Sadiq, N. A. , Emikpe, B. O. , Omobowale, T. "Assessment of cutaneous and serum oxidative stress changes in dogs infested with Sarcoptes scabiei var canis". Acta Veterinaria Eurasia (2019 ): 0-0
RIS TY - JOUR T1 - Assessment of cutaneous and serum oxidative stress changes in dogs infested with Sarcoptes scabiei var canis AU - Onyeka Chidiebele Nwufoh , Nurudeen Ayinde Sadiq , Benjamin Obukowho Emikpe , Tayo Omobowale Y1 - 2019 PY - 2019 N1 - DO - T2 - Acta Veterinaria Eurasia JF - Journal JO - JOR SP - 0 EP - 0 VL - IS - SN - 2618-639X-2619-905X M3 - UR - Y2 - 2022 ER -
EndNote %0 Acta Veterinaria Eurasia Assessment of cutaneous and serum oxidative stress changes in dogs infested with Sarcoptes scabiei var canis %A Onyeka Chidiebele Nwufoh , Nurudeen Ayinde Sadiq , Benjamin Obukowho Emikpe , Tayo Omobowale %T Assessment of cutaneous and serum oxidative stress changes in dogs infested with Sarcoptes scabiei var canis %D 2019 %J Acta Veterinaria Eurasia %P 2618-639X-2619-905X %V %N %R %U
ISNAD Nwufoh, Onyeka Chidiebele , Sadiq, Nurudeen Ayinde , Emikpe, Benjamin Obukowho , Omobowale, Tayo . "Assessment of cutaneous and serum oxidative stress changes in dogs infested with Sarcoptes scabiei var canis". Acta Veterinaria Eurasia / (January 2019): 0-0 .
AMA Nwufoh O. C. , Sadiq N. A. , Emikpe B. O. , Omobowale T. Assessment of cutaneous and serum oxidative stress changes in dogs infested with Sarcoptes scabiei var canis. Actavet. 2019; 0-0.
Vancouver Nwufoh O. C. , Sadiq N. A. , Emikpe B. O. , Omobowale T. Assessment of cutaneous and serum oxidative stress changes in dogs infested with Sarcoptes scabiei var canis. Acta Veterinaria Eurasia. 2019; 0-0.
IEEE O. C. Nwufoh , N. A. Sadiq , B. O. Emikpe and T. Omobowale , "Assessment of cutaneous and serum oxidative stress changes in dogs infested with Sarcoptes scabiei var canis", Acta Veterinaria Eurasia, pp. 0-0, Jan. 2019