Clinicopathological assessment of Infectious bursal disease vaccine using phytogenic mucoadhesive agents in challenged broilers

Year 2019, , 0 - 0, 01.01.2019

Adeniran Ganiyu Adetunji Jarikre Theophilus Aghogho Ola Olawale Olawumi Ohore Obokparo Godspower Oyebanji Victor Oyewale Emikpe Benjamin Obukowho

Abstract

The loss due to disharmonized vaccination against infectious bursa disease (IBD) has necessitated the use of plant gums in poultry production. This study evaluated oral administration of IBD vaccine with equal proportions of Cedrella odorata and Khaya senegalensis in chickens. Two hundred and forty chicks of four groups; Mucilages-IBD Vaccine (virus challenged) (GGVOR), IBD Vaccine alone (virus challenged) (GVOR), Mucilage alone (virus challenged) (GOR), and Not Vaccinated-No mucilae (virus challenged) (NVNG/C). The birds were monitored clinically post vaccination and infection; lymphoid tissues were taken from weak euthanized birds for gross and microscopic examination. The clinical data and lesion scores from the tissues were analysed and compared at α=0.05. All the birds were apparently healthy pre and post vaccinations. A few days post infection there were signs of pecking, soiling of the vents and feathers with projectile yellowish diarrhoea and varied mortalities in the GOR and NVNG/G birds. The bursa of Fabricius, spleen, caecal tonsil and Harderian gland were oedematous, hyperplastic, haemorrhagic and inflamed to various degree across the groups but very severe in NVNG/C birds. No mortality was recorded in GVVOR and GVOR. Administration of IBDV vaccines with Cedrella odorata and Khaya senegalensis gums clinically enhanced the birds against IBD.

Cite this article as: Adetunji, A.G., Aghogho, J.T., Olawumi, O.O., Godspower, O.O., Oyewale, O.V., Obukowho, E.B., 2020. Clinicopathological assessment of Infectious bursal disease vaccine using phytogenic mucoadhesive agents in challenged broilers. Acta Vet Eurasia, DOI: 10.5152/actavet.2020.19020

Keywords

Chicks, IBDV, Mucilages, Oral, Immune response

References

• Adedokun, S., Sonaiya, E. 2001 Comparison of the performance of Nigerian indigenous chickens from three agro-ecological zones. Livestock Res Rural Devel 13 (2): 132-136 Adene, D.F. 2008. Poultry health and production, principles and practices. Stirling-Horden Publishers Ltd. Pp 68. Adene, D.F., Oguntade, A.E. 2006. The structure and importance of the commercial and village based poultry industry in Nigeria. FAO (ROME) Study October 2006. Pp 109 Ajala, M.K., Nwagu, B.I., Otchere, E.O. 2007. Socio-economics of free range poultry production among agropastoral women in Giwa local government area of Kaduna state, Nigeria. Nig Vet J 28 (3): 11-18 Alders, R.G., Pym, R.A.E. 2009. Village poultry: still important to millions, eight thousand years after domestication. World's Poultry Sci J 65:181-190 Ayorinde, J.O., Odeniyi, M.A. 2012. Material and rheological properties of yam starch/polymer blend hydrogels. Int J Pharm Pharm Technol 1: 47. Bell, J.G. 2000. A Comparison of the Different Vaccines Available for the Control of Newcastle Disease in Village Chickens. In: Alders R.G and Spradbrow P.B; PR103, SADC planning workshop on Newcastle disease control in village chickens. A workshop in Maputo, Mozambique 6-9 March, 2000 on Newcastle disease in village chickens. aciar.gov.au/files/node/2131/pr103chapter14.pdf. 2001. Bhosale, R.R., Osmani, R.A.M., Moin, A. 2015. Natural Gums and Mucilages A Review on Multifaceted Excipients in Pharmaceutical Science and Research. Inter. J. of Pharmacog. Phytochem. Res 6(4): 901- 912. de Troconis, G.N., Martinez, M., de Pinto, L.G., Bhasas, A. 2001. Estudio químico y espectroscó pico del polisacá rido de la goma de Cedrela odorata. Ciencia 9: 235–3. Emikpe, B.O., Akpavie, S.O., Adene, D.F. 2001. Influence of Parenteral Route on Oral Route of Local IBD Vaccine Administration in the Responses of Broiler Chicks. Revue Élev Méd vét Pays trop 54 (3-4): 213-216 Emikpe, B.O., Oyebanji, V.O., Odeniyi, M.A., Salaam, A.M., Oladele, O.A., Jarikre, T.A., Akinboade, O.A. 2016. Ex-vivo evaluation of the mucoadhesive properties of Cedrela odorata and Khaya senegalensis gums with possible applications for veterinary vaccine delivery. Springerplus (5): 1289. doi:10.1186/s40064-016-2948-0. Mack, S., Hoffmann. D., Otte, J. 2005. The contribution of poultry to rural development. World’s Poultry Sci J 61: 7-14 Nyoni, N.M.B., Masika, P.J. 2012. Village chicken production practices in the Amatola Basin of the Eastern Cape Province, South Africa. African J Agric Res 7(17): 2647-2652. Odeniyi, M.A., Khan, N.H., Peh, K.K. 2015. Release and mucoadhesion properties of diclofenac matrix tablets from natural and synthetic polymer blends. Acta Polo Pharma – Drug Res 72: 559-7. Odeniyi, M.A., Takeuchi, H. 2014. Design and evaluation of cedrela gum based microparticles of theophilline. Ars Pharmaceutica 55(1):30-36. OIE terestial manual 2016. Infectious bursal disease Chapter 2.3.12 pp 21 Okwor, E.C., Eze, D.C., Uzuegbu, O.M. 2013. Comparative studies on the oral and intraocular routes of administration of Newcastle disease vaccine, La Sota in adult chickens. J Agric Vet Sci 3:48–51. Oyebanji, V.O., Emikpe, B.O., Oladele, A.O., Odeniyi, M.A., Salami, A., Osowole, O.I., et al 2016. Evaluation of immune response in challenged chickens vaccinated with Newcastle disease vaccine using gums from Cedrela odorata and Khaya senegalensis as delivery agents. J Immunoass Immunochem 23:1–11. Oyebanji, V.O., Emikpe, B.O., Oladele O,A., Osowole O,I., Salaam, A., Odeniyi, M.A., Kasali, O.B., Akinboade, O.A. 2017. Clinicopathological evaluation of Newcastle disease virus vaccination using gums from Cedrela odorata and Khaya senegalensis as delivery agents in challenged chickens. Int J Vet Sci Med 5:135–142 Peralta, M.F., Danelli ,M.G.M., Vivas, A. 2016. Rediscovering the importance of Mucosal Immune System (MIS) in poultry. Acad J Biotechnol 4:91–5. Petrovsky, N., Aguilar, J.C. 2004. Vaccine adjuvants: Current state and future trends. Immunol Cell Bio 82: 488–496. doi:10.1111/j.1440-1711.2004.01272. Singh, J., Banga, H.S., Brar, R.S., Singh, N.D., Sodhi, S., Leishangthem, G.D. 2015 Histopathological and immunohistochemical diagnosis of infectious bursal disease in poultry birds. Vet World 8(11): 1331- 1339.