Abstract

Amaç: Bu çalışmanın amacı, zaman alıcı adeziv prosedürleri kısaltan self-adeziv akışkan rezin kompozit (Vertise Flow,Kerr Corp, Orange, CA, ABD) ekstrelerinin potansiyel mutajenik etkisinin değerlendirilmesidir. Çalışma dizaynı: Materyal örnekleri dimetil sülfoksit içerisinde bekletilmişler ve 370C’ deki inkübasyon sürelerinin 1. ve 7. günlerinde test edilmişlerdir. Bu materyallerin mutajenik etkileri, Salmonella typhipmurium TA 100 suşunda, standart plak korporasyon yöntemi ile S9 fraksiyonu eksikliğinde değerlendirilmiştir. Veriler iki yönlü varyans analizi yardımı ile istatistiksel olarak analiz edilmiştir (p<0.05). Bulgular: Materyal dozu ve inkübasyon süresi ve birbirleri ile ilişkileri Salmonel-la typhipmurium koloni sayıları üzerinde farklı etkiler göstermiştir. Bununla birlikte self-adeziv restoratif materyal için mutajenik etki gözlenmemiştir. Sonuç: Bu çalışmada test edilen adeziv restoratif materyalin mutajenik etkisi bulunmamıştır.

Keywords

• mutajenisite, AMES testi, Salmonella tphymiruim, self-adeziv kompozit, bulk fill akışkanlar, adeziv rezin

MUTAGENIC POTENTIAL OF A SELF-ADHESIVE FLOWABLE COMPOSITE

Abstract

Objective: The aim of this study was to assess the potential mutagenic effects associated to extracts from current self-adhesive flowable resin composite (Vertise Flow,Kerr Corp, Orange, CA, USA) that allows skipping the time-consuming adhesive processes. Study design: The materials were eluted in dimethyl sulphoxide and the extracts were tested either after 1 day or 7 day incubation period at 370C. Mutagenic effects of the materials were tested on Salmonella typhipmurium strain TA 100 using the standard plate incorporation assay in the absence of S9 fraction from rat liver. The data were statistically analyzed using twoway variance analysis (p<0.05). Results: The dose of the material and incubation as well as the interactions between these factors exhibited varying degrees of influences on the Salmonella typhipmurium colony number. However no mutagenic effect was detected for the self-adhesive restorative material. Conclusion: It can be concluded that the adhesive restorative material tested in this study has no mutagenic potential.

Keywords

• mutagenicity, AMES test, Salmonella tphymiruim, self-adhesive composite, bulk fill flowables, adhesive resin

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