Detection of Tilidine, Nortilidine and Bisnortilidine in Dried BIoodstains

Abstract

Low nanogram amounts of the narcotic analgesic tilidine and its two main metabolites nortilidine and bisnortilidine were detected in dried stains originating from 100 to 250 microlitre blood. Treatment of the bloodstains and final detection of the three substances, which was performed by capillary gas chromatography with nitrogen-phosphorus detection, were described. By means of the proposed procedure tilidine and its two metabolites were still detectable at the therapeutic level. The detectability and the stabilities of the compounds studied, as a function of the age of the bloodstains, was also investigated.

Keywords

• tilidine
• nortilidine
• dried bloodstain

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