Studies on in vitro Hemolytic Effect of Morphine Sulphate

Abstract

In vitro studies on hemolytic effect of morphine sulphate (MS) has been carried out on rat and human erythrocytes and are found to be susceptible to hemolysis. Comparatively human erythrocytes are most sensitive to hemolysis by MS. The degree of hemolysis is found to be dose dependenl. The depletion of glutathione and the increase in lipid peroxidation may be responsible for the disintegration of erythrocyte membrane. Erythrocytes preincubated with glucose and A TP proteet the celis against morphine induced lysis.

Keywords

• Morphine sulphate,
• Glucose and ATP
• Hemolytic effect

References

1. JJ-H. Jaffe, W.R. :\fanin (1980) in The Pharmacoiogica/ Basis of Therapeutics, 2nd cdn (A.G. Gilman, LS. Goodman, A.Gilman, ed.) pp.494-S20, ~v1ucmiııalı Publi;;huıg Co .• New York.
2. Feldbcrg, W., Shaligram, S.V. (1972) B,. 1. Phamuıcol., 46, 602-618.
3. Trotta, R.J., Sullivan, S.G., Stern, A., (1983) Biochem. f.,212, 759-773.
4. Fiske, C.H., Suhbarow, Y. (1925) IBiol. Chem, 66, 375-38 L
5. Parekh, A.C., Jung, D.H. (19'10) AnaL. Chem, 42, 1423-1436.
6. Warren, L (1959) J. Bio/. C/um, 234, 234-242.
7. Ohakawa, H., Obı.sni, N., Yagi, M. (1979) Anal. Biochem.,9S, 351-372.
8. Moron. M.S., Depterre, .ı.W., Manneröik, (1979) Rıochim. Biophys. Acla ,582, 67-74.

9. Drabkin, D.L, Austin, J.H. (1935) J. Biol. Chem., 112, 51-62.
10. Nci!, A.L, Charles. R.Z. (1988) Biochim. Biophys. ACla, 964, 96-104. J ı Brody, A.R., Gerwyn, G., Lila. H. (1983) Lab. ["vest., 49, 468-474.
11. Cooper, R.A.~ Durochcr. J.R., Le.lic, MJ!. (1977) J. Clin. Chem., 60. ı 15-121.
12. Plaa. G.L, Wiıselıi, ıı. (1976) Ann. Rev. Pharmacol. Toxicol., 16, 125-141.