EvaIuation of Hepatitis-B Surface Antigen As a Forensic Marker

Year 1990, Volume: 6 Issue: 1-2, 16 - 21, 30.04.1990

D. V. Rao

https://izlik.org/JA44WN33RR

Abstract

The presence of extraneous antigens in blood may be of unique forensic importance in discriminating source among identical twins and genetically dose related individuals. Hepatitis surface antigen (HBsAg) as a forensic marker in establishing the source of blood stains has been evaluated by studying the stability of HBsAg in stains and its frequency in Hyderabad population. Microstrip based double anlibody sandwich ELlSA is employed to detect HBsAg in blood stains. HBsAg was found to be stable up to 5 week s in blood stains exposed to ambient condilions.

Keywords

HBsAg , ELISA , Storage , Stability , Frequency

References

• Levkov, J.S. (1987) i Forensic Sci., 32,357-363.
• Tahir, MA (1989) Sci.lnl. (Lahore), 1,281-282.
• Tahir, MA, Brown, M.L. (1984) i Forensic Sci., 29,660-662.
• Keller, P.J., Kuufman, A.D., Allan, B.J., Williams, B.L. (1971) Biochem., LO, 4867-4874.
• Netrün, M.S., Tumer, L.L., Reisher, E.G. (1983) f. Forensic Sci., 28,608-614.
• Waye, J.S., Presley, L.A., Budowle, B., Shutter, G.G., Fourney, R.M. (1989) Biotechniques, 7, 852-855.
• Kashyap, V.K., Rao, D.V. (1989) Application of extraneous serological markers in forensic investigation, in Proceeding of Short-term Course in Immunological Methods in Forensic Investigation, Nov. 6-10.
• Inconescu, M. (1985) i Immunol. Melhods, 76, 307-316.
• Buckleton, J.S., Walsh, K. (1987) i Forensic Sci. Soc., 27, 103-112.
• Grunbaum, B.W., Selvin, S., Pace, N., Black, D.M. (1978) f. Forensic Sci., 3,577-587.
• Peterson, A. (1981) i Biochem., 256,6975.
• Jawetz, E., Melnick, J.L., Adelberg, E.A. (1987) in Review of Medical Microbiology, pp. 445, Prentice-Hall International, Ine., U.S.A.
• Blurnerg, B.S. (1977) Science, 197, 17.
• Zuckerman, A.J. (1981) f.lnfecı. Dis., 143,301.
• Lander, J.J., Alfer, H.I., Purcell, R.H. (1971) f. Immuno!., 106, 1166.