Analysis of Opiates in Human Hair With FPIA, EMIT, and GC/MS

Year 1991, Volume: 7 Issue: 3-4, 114 - 117, 31.08.1991

P. Kintz

https://izlik.org/JA29LZ58XE

Abstract

The authors evaluate use of intmunoassay techniques (EMIT, FPIA) and gas chromatography coupled to mass spectrometry in estimation of chronic opiate abuse using human hair. Samples were obtained from 18 heroin abusers and decontaminated in 5 ml of dichloromethane for 15 min at 37°C and then incubated in 1 mlof 1 M NaOH for 10 min at 100°C. Afterwards, the aliquots were neutralized with 1 ml of 1 MHO,
centrifugated and directly analyzed using the Abbott ADx, the Syva ETS Plus and GC/MS. No false response was obtained with the Abbott ADx, by comparison with GC/MS, clea rly demonstrating the capability of ADx for opiate screening of human hair. This was not the case for EMIT, since a great number of false negative responses was observed.

Keywords

Human hair , EMIT , FPIA

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