The Sequential Determination of Glyoxalase-I Isoenzymes and ABO (H) Antigens From the Same Piece of Menstrual Blood Stain Thread

Abstract

Fifty paired stains of normal and menstrual blood have been analysed for the sequential determination of GLO-I isoenzymes and ABO(H) antigens from the same piece of cloth. It has been observed that ABO (H) antigens could be successfully determined after GLO-I isoenzyme typing, from the same fragment of the menstrual blood stains (98 per cent). The treatment of stains with reducing agent prior to inse rtion did not affect the results and the similar type of isoenzymes patterns were observed in the paired stains of the same indi vidual. The GLO-I isoenzyme typing were detected for a longer period in stains stored at deep free ze r as compared to room temperature. The GLO-I isoenzyme typing and ABO (H) antigens detection could be performed on the same thread particularly when the biological material submitted for forensic examination is limited.

Keywords

• Glyooxalase I
• ABO (H) Antigens
• Menstrual blood stain

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