The Sequential Determination of Glyoxalase-I Isoenzymes and ABO (H) Antigens From the Same Piece of Menstrual Blood Stain Thread

Year 1992, Volume: 8 Issue: 1, 57 - 62, 30.04.1992

Rakesh Kumar Garg

https://izlik.org/JA49TU52SU

Abstract

Fifty paired stains of normal and menstrual blood have been analysed for the sequential determination of GLO-I isoenzymes and ABO(H) antigens from the same piece of cloth. It has been observed that ABO
(H) antigens could be successfully determined after GLO-I isoenzyme typing, from the same fragment of the menstrual blood stains (98 per cent). The treatment of stains with reducing agent prior to inse rtion did not affect the results and the similar type of isoenzymes patterns were observed in the paired stains of the same indi vidual. The GLO-I isoenzyme typing were detected for a longer period in stains stored at deep free ze r as compared to room temperature. The GLO-I isoenzyme typing and ABO (H) antigens detection could be performed on the same thread particularly when the biological material submitted for forensic examination is limited.

Keywords

Glyooxalase I , ABO (H) Antigens , Menstrual blood stain

References

• Culliford, BJ. (1959) Journal of Forensic Medicine , 6, 112-115.
• Newall, PJ. (1969) Proc. Vth International Meeting of the Forensic Sciences, Toronto, Canada.
• Kamimura, O. (1961) Acto Med. Okayalllll, 15, 315-326.
• Ota, S., Furaya, Y., Fujii, K. (1965) Japonica Journal (it Legal Medicine, 19, 300-305.
• Asano, M., Pya, M., Masayohi, H. (1972) Forensic Sciences, 4,53-61.
• Whitehead, P.R., Divali, G.B. (1973) Clinical Chemistrv, 19,762-765.
• Whitehead, P,H., Divali, G.B. ( 1974) Forensic Sciences, 4, 53-6 I.
• Suryakala, A. ( ) Differentiation of Menstrual Bloodfrom other blood slains and their groupingji)r ABO (H) and Rh (D) groups. Ph.D. Thesis suhmitted to Punjabi University, Patiala, (unpublished).
• Kaur, G., Sharma, V.K. (1985) Annals, BioI., 1,75-79.
• Whitehead, P.H., Breck, A. (1974) Journal of' Forensic Science Societ y, 14, 109-110.
• Khalap, S., Divali, G.B. (1978) Forensic Sciences, 11 ,4 1-45.
• Divali, G.B., Khalap, S. ( 1979) Journal of Forensic Sciences, 14, 104- 105.
• Adamo, R., Kobilinsky, L. (1984) Journal of' Forensic Sciences, 29, 436-444.
• Budowlc, B. (1985) Journal oj Forellsic Sciences, 30, 12 16-1220.
• Lee, H.C, Gaensslen, RE, Pagliaro, E.M., Lee, S.K., Carreli, RJ. (1985) Forensic Science Internationa l, 29. 191-198.
• Wraxall, B.G.D., Stolorow, M.D. (1986) journal oj Forensic Sciences, 31 , 1439- 1449.
• Garg, R.K. , Kobilinsky, L. (1989) journal of Forensic Sciences, 34,336-345 .
• G arg, R,K" Dhindsa, A.S. ( 1989) Indian journal oj Forensic Science, 3, 11 7- 121.
• DUllsford, I., Bowley, c.c. (1967) Techniques in Blood grouping, Vol. I & II. Oliver and Boyd, Edinburgh.
• Kin d, S.S, (1960) AiJsorption-elution grouping ojdried Nood stains (ll/faiJ rics, Nature, London, Vol. 187, 789-790.
• Scott, A.C., Fowler, J.C.S. (1982) Forensic Science IntemaTional, 20, 287-294.
• Ghosh, A,K. (1977) Hum. Gen., 39,91 -95.
• Sehajpal, P.K., Mehta, K., Sharma, V.K., Sandhu, H.S. (1983) Proe. Xth International Congress of the Society of Forensic Haemogenetics, held at Munich.
• Sehajpal, P.K. , Tewari, K., Bhanwar, AJ.S. (1984) Abstract, 531 , American journal of" HUlllan Gel/e/ics, 36, 1795,
• Stohlmacher, P., Hafcrland, W, (1980) Zei/schriji Jur Rechlsmedbn, 85, 165,
• Kallr, G. (1985) Some Observations on the GLO-J Polymorphism in Menstrual Blood Stain. M.Se. Special Report submitted to Punjabi University, Patia!a.