EROİN, MORFİN, KOKAİN VE MDMA’NIN YÜKSEK PERFORMANSLI İNCE TABAKA KROMATOGRAFİSİ İLE EŞZAMANLI TAYİNİ

Abstract

Gıda, çevre, ilaç, bitki ve metabolizma gibi pek çok alanda yaygın olarak kullanılan geleneksel İnce Tabaka Kromatografisi (İTK)’ nin standardize edilmesi çerçevesinde son yıllarda teknolojideki yeniliklerle bütünleşerek Yüksek Performanslı İnce Tabaka Kromatografisi (YPİTK) sistemi geliştirilmiştir. Bu sistem, eş zamanlı olup aynı plakada ayırım sağlayarak, direkt densitometrik ölçümler ile miktar analizini mümkün kılmaktadır. Bu çalışma, ülkemizde sık rastlanan psikotrop maddelerin tanımlanması amacıyla oluşturulmuş bir yöntem bildirilmekte olup uygulama birimi olarak CAMAG ATS4, geliştirme birimi olarak CAMAG ADC2 ve tarama birimi olarak CAMAG TLC Scanner3 ile gerçekleşmiştir. Eroin, morfin, kokain, ve metilendioksimetamfetamin (MDMA)’in Rf değerleri Toluen: Aseton: Etanol: NH3(25%) (67:25:5:3) mobil sisteminde sırasıyla 0.41, 0.18, 0.66, 0.33 bulunmuştur. Standart maddelerin metanol içinde belirleme limiti (LOD) değerleri sırasıyla 6, 8, 0.1, 1.6 ng/µL ve ölçüm limiti (LOQ) değerleri sırasıyla 18, 24, 0.3, 4.8 ng/µL olarak hesaplanmıştır. Bu çalışma, YPİTK metodunun, psikotrop maddeler için, basit, hassas, kesin ve doğru miktar analizi sağladığını göstermiştir. Her birinin bileşiminin belirlenmesi yoluyla orijin tespitinde de kullanılabileceği hassaslıktaki bu yöntem ile kriminal vakalarda ele geçen psikotrop maddelerin, madde ile kirletildiği şüphesindeki yüzey ve materyallerin analizlerinin incelenebileceği düşünülmektedir

Keywords

• ypitk analizi
• kantitatif analiz
• adli kimya

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