Effect of Different MMP Inhibitors on the Bond Strength and Durability of an Etch-and-rinse and a Self-etch Adhesive

Year 2024, Volume: 13 Issue: 3, 453 - 460, 24.09.2024

Ahmet Hazar , Mine Betül Üçtaşlı

https://doi.org/10.54617/adoklinikbilimler.1485108

Abstract

Aim: To compare the effect of different matrix metalloproteinase (MMP) inhibitors on the microtensile bond strength (μTBS) of an etch-and-rinse adhesive and a self-etch adhesive at baseline and after aging. Materials and Method: Flat dentin surfaces were prepared from 96 extracted, sound human molars. Teeth were randomly divided into two main groups according to the adhesives (Adper Single Bond 2, and Clearfil 3S Plus) used (n=48). Each group was divided into four subgroups according to the dentin surface treatments; untreated (control), pretreated with 2% chlorhexidine digluconate (CHX) before adhesive application, pretreated with 1% benzalkonium chloride (BAC) before adhesive application, and pretreated with 2% hesperidin (HPN) before adhesive application (n=12). Composite resin was then placed on the prepared surfaces and polymerized. The specimens were cut to obtain dentin beams and randomly divided into two groups for microtensile analysis at baseline and after 6 months of aging. Failure types were observed test after microtensile tests and data were analyzed by Two-way ANOVA, Tukey HSD, and Chi-Square tests (p <0.05). Results: Compared with control, all MMP inhibitors significantly preserved and improved the bond strength of both adhesives at baseline and after 6 months of aging (p < 0.05). When considering the comparison of MMP inhibitors, bond strengths of both adhesives in the HPN and BAC groups were significantly higher than CHX group after aging (p < 0.05). The failure types were mostly adhesive and mixed. Conclusion: The use of 2% HPN and 1% BAC as a separate step before the adhesive application can be recommended to preserve the longevity of resin-dentin bond strength on etch-and-rinse and self-etch adhesives.

Keywords

Adhesives, Benzalkonium chloride, Chlorhexidine digluconate, Hesperidin

Ethical Statement

Ankara University Faculty of Dentistry Clinical Research Ethics Committee, 21.01.2015, protocol number: 36290600/09.

Supporting Institution

This study was supported by the department of scientific research projects, Gazi University, project number: 03/2015-10

Project Number

This study was supported by the department of scientific research projects, Gazi University, project number: 03/2015-10

Farklı MMP İnhibitörlerinin Etch-and-rinse ve Self-etch Adezivin Bağlanma Kuvveti ve Dayanıklılığı Üzerine Etkisi

Abstract

Amaç: Farklı matriks metalloproteinaz (MMP) inhibitörlerinin, başlangıç ve yaşlandırma sonrasında bir pürüzlendirmeli ve yıkamalı adeziv ile bir kendinden pürüzlendirmeli adezivin mikrogerilim bağlanma kuvveti (μTBS) üzerindeki etkisini karşılaştırmak. Gereç ve yöntem: 96 adet çekilmiş sağlam insan azı dişinden düz dentin yüzeyleri hazırlandı. Dişler kullanılan adezivlere (Adper Single Bond 2 ve Clearfil 3S Plus) göre rastgele iki ana gruba ayrıldı (n=48). Her grup dentin yüzey işlemlerine göre dört alt gruba ayrıldı; işlem uygulanmayan (kontrol), adeziv uygulanmadan önce %2 klorheksidin diglukonat uygulaması (CHX), adeziv uygulanmadan önce %1 benzalkonyum klorür (BAC) uygulaması ve adeziv uygulanmadan önce %2 hesperidin (HPN) uygulaması (n=12). Daha sona hazırlanan yüzeylere kompozit rezin yerleştirilip polymerize edildi. Örnekler, dentin çubukları elde etmek için kesildi, başlangıç ve 6 aylık yaşlandırmadan sonra mikrotensil analizi için rastgele iki gruba ayrıldı. Mikrogerilim testleri sonrasında kırık tipleri gözlemlendi ve veriler Two-way ANOVA, Tukey HSD ve Ki-Kare testleri ile analiz edildi (p <0.05). Bulgular: Kontrol ile karşılaştırıldığında, tüm MMP inhibitörleri başlangıçta ve 6 aylık yaşlandırmadan sonra her iki adezivin bağlanma kuvvetini önemli ölçüde korumuş ve iyileştirmiştir (p < 0.05).MMP inhibitörlerinin karşılaştırılması göz önüne alındığında, HPN ve BAC gruplarındaki her iki adezivin bağlanma kuvvetleri, yaşlandırma sonrasında CHX grubundan önemli ölçüde daha yüksekti (p < 0.05). Başarısızlık türleri çoğunlukla adeziv ve karışık olarak görüldü. Sonuç: Pürüzlendirmeli ve yıkamalı, kendinden pürüzlendirmeli adezivlerde rezin-dentin bağlanma kuvvetinin uzun ömürlülüğünü korumak için adeziv uygulamasından önce ayrı bir adım olarak %2 HPN ve %1 BAC kullanımı önerilebilir.

Keywords

Adezivler, Benzalkonyum klorür, klorheksidin diglukonat, hesperidin

Project Number

This study was supported by the department of scientific research projects, Gazi University, project number: 03/2015-10

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