Research Article
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Investigation of Canine Distemper Virus Infection in Dogs in the Antalya Province

Year 2021, , 45 - 51, 24.12.2021
https://doi.org/10.53913/aduveterinary.1000408

Abstract

Canine distemper virus is one of the most prevalent infectious agents causing severe clinical symptoms among canids. Communal life-habitat for example clinics, dog-shelters, or rural areas is critical in terms of the transmission dynamics of CDV. In this study, blood samples from 92 dogs, aged 2 to 12 months, that were brought to the Rehabilitation Center and private veterinary clinics in Antalya with various internal medical problems were examined for Canine Distemper Virus (CDV) infection. Samples were tested using commercial immunochromatographic rapid test for detection of CDV-antigens and Enzyme Linked Immunosorbent Assay (ELISA) for detection of CDV-specific IgG and IgM antibodies.
The most common clinical findings in the 92 dogs sampled were mucopurulent discharge in the eye (45.65%), nasal hyperkeratosis (35.87%), nasal mucopurulent discharge (25%), cough (13.04%), diarrhea (8.70%), and fatigue (6.52%). It was observed that clinical findings were more intense in the early reconvalescent period. 5.43% (5/92) of the samples examined by immunochromatographic rapid test were positive. The positivity rates of IgG and IgM antibodies by ELISA were 19.56% (18/92) and 94.56% (87/92), respectively. Of the 92 dogs tested by ELISA for detection of IgG and IgM antibodies, 91 (98.91%) were positive for one or both IgG or IgM antibodies, and 1 (1.09%) was negative for both antibodies.
In conclusion, it was determined that CDV infection is actively circulating in the Antalya province and poses a risk for unvaccinated dogs in the region.

Supporting Institution

Adnan Menderes Üniversitesi, Bilimsel Araştırma Projeleri

Project Number

VTF-18020

Thanks

Acknowledgements This study was supported by Foundation of Scientific Research Projects of the Aydin Adnan Menderes University with project number VTF-18020. The paper was summarised from a Master’s thesis of the first author.

References

  • Anis, E., Holford, A.L., Galyon G.D., & Wilkes R.P. (2018). Antigenic analysis of genetic variants of Canine distemper virus. Veterinary Microbiology, 219, Pages 154-160.
  • Appel, M.J.G., & Summers, B.A., (1999). Canine distemper: Current Status. Recent Advances in Canine Infectious Diseases. Erişim:[ http://www.ivis.com.]
  • Barrett, T. (1999). Morbillivirus infections with special emphassis on morbilliviruses of carnivores. Veterinary Microbiology, 69, 3-13.
  • Beineke, A, Puff, C, Seehusen, F, & Baumgärtner, W. (2009). Pathogenesis and immunopathology of systemic and nervous canine distemper, Veterinary Immunology and Immunopathology, 127, 1-18.
  • Blixenkrone-Møller, M., Svansson, V., Have, P., Orvell, C., Appel, M., Rode Pedersen, I., & Henriksen, P. (1993). Studies on manifestations of canine distemper virus infection in an urban dog population. Veterinary Microbiology, 37(1-2), 163–173.
  • Budaszewski R. (2016). Influence of vaccine strains on the evolution of canine distemper, Infection, Genetics and Evolution, 41, 262-269.
  • Çalışkan E, & Burgu I. Köpek gençlik hastalığı virusunun prevalansı ve seroepidemiyolojisi. Veterinary Microbiology 2007, 18, 5-10.
  • Çalışkan E. (2007). Köpek Gençlik Hastalığı Virus İzolasyonu, H Proteini kodlayan gen bölgesinin karakterizasyonu ve seroepidemiyolojisi, Doktora Tezi, Ankara Üniversitesi, Sağlık Bilimleri Enstitüsü, Ankara.
  • Çalışkan, E, & Burgu, I. (2007). Köpek gençlik hastalığı virusunun prevalansı ve seroepidemiyolojisi. Veterinary Microbiology, 18, 5-10.
  • Damián, M., Morales, E., Salas, G., & Trigo, F. J. (2005). Immunohistochemical Detection of Antigens of Distemper, Adenovirus and Parainfluenza Viruses in Domestic Dogs with Pneumonia. Journal of Comparative Pathology 133(4), 289–293.
  • Dorji, T., Tenzin, T., K. Tenzin, K., Tshering, D., Rinzin, K., Phimpraphai, W., & de Garine-Wichatitsky, M (2020). Seroprevalence and risk factors of canine distemper virus in the pet and stray dogs in Haa, western Bhutan. BMC Veterinary Research, 16, 135. https://doi.org/10.1186/s12917-020-02355-x.
  • Elia, G., Camero, M., Losurdo, M., Lucente, M. S., Larocca, V., Martella, V., Decaro, N., & Buonavoglia, C. (2015). Virological and serological findings in dogs with naturally occurring distemper. Journal of Virological Methods. 213, 127-130.
  • Esin, E. (2013). Köpeklerde Canine Distemper Virus Enfeksiyonun Araştırılması, Doktora Tezi, Konya Selçuk Üniversitesi, Sağlık Bilimleri Enstitüsü, Konya.
  • ICTV (2020). International Committee on Taxonomy of Viruses. https://talk.ictvonline.org/taxonomy/ (accessed 20 August 2021).
  • Koutinas, A.F., Baumgärtner, W., Tontis, D., Polizopoulou, Z., Saridomichelakis, M.N., & Lekkas, S. (2004). Histopathology and immunohistochemistry of canine distemper virus-induced footpad hyperkeratosis (hard Pad disease) in dogs with natural canine distemper. Veterinary Pathology, 41, 2-9.
  • Latha D, Geetha M, Ramadass, P., & Narayanan, R.B. (2007). Development of recombinant nucleocapsid protein based IgM-ELISA for the early detection of distemper infection in dogs. Veterinary Immunology and Immunopathology, 119, 278-286.
  • Latha, D., Srinivasan, S.R., Thirunavukkarasu, P.S., Gunaselan, L., Ramadass, P., Narayanan, R.B. (2007). Assessment of canine distemper virus infection in vaccinated and unvaccinated dogs. Indian Journal of Biotechnology, 6, 35-40.
  • Nguyen Thi, The Growth Profiles of Three Types of Canine Distemper Virus on Vero Cells, The Journal of Veterinary Medical Science 2005,67(5):491-495.
  • Panzera, Y., Sarute, N., Iraola, G., Hernández, M., & Pérez, R. (2015). Molecular phylogeography of canine distemper virus: Geographic origin and global spreading. Molecular Phylogenetics and Evolution, 6-15.
  • Pardo, M.C., Tanner, P., Bauman, J., Silver, K., & Fischer, L. (2007). Immunization of puppies in the presence of maternally derived antibodies against canine distemper virus. Journal of Comparative Pathology, 137, 72-75.
  • Richmann, D.D., Whitley R.J., & Hayden, F.G. (2009). Clinical virology, 4th edition, American Society for Microbiology. Washington, 1489.
  • Rikula UK. Canine distemper in Finland-vaccination and epidemiology, Academic Disertation, University of Helsinki, Finland, 2008.
  • Sabatino, D. (2016). Lethal distemper in badgers (Meles meles) following epidemic in dogs and wolves, Infection, Genetics and Evolution, 46,130-137.
  • Saltık H.S.(2018). Klinik semptomlu köpeklerin kan, idrar, rektal sürüntü, nasal ve oküler akıntı örneklerinde canine distemper virus enfeksiyonun real time RT-PCR ile tespit edilmesi. Doktora Tezi, Mehmet Akif Ersoy Üniversitesi, Sağlık Bilimleri Enstitüsü, Burdur.
  • Saltık, H.S., Kale, M. (2020). Evaluation of infection with N protein-specific Immunoglobulin M and G in naturally occurring distemper in dogs. Veterinarni Medicina, 65, 2020 (04), 168–173. https://doi.org/10.17221/31/2019-VETMED.
  • Sugai A, Kooriyama T, Sato H, Yoneda M, & Kai C. (2009). Epitope mapping of Canine distemper virus phosphoprotein by monoclonal antibodies. Microbiology and Immunology., 53, 667-674.
  • Şahna KC, Gençay A, & Atalay O. Viral etilogy of diarrhoea in puppies from a same shelter in turkey, presence of mixed infections. Revue de Médecine Vétérinaire 2008, 159, 345-347.
  • Terio, K.A., & Craft ME. Canine distemper virus (CDV) in another big cat, should CDV be renamed carnivore distemper virus? Microbiology. 2013, 4, 702-713.
  • Vivaldo, G. (2019). Molecular and serological surveys of canine distemper virus: A meta-analysis of cross-sectional studies, PLoS One. 14. https://doi.org/10.1371/journal.pone.0217594.
  • Von Messling, V., Harder T.C., Moennig, V., Rautenberg, P., Nolte, I., & Haas, L. (1999). Rapid and sensitive detection of ımmunoglobulin M (IgM) and IgG antibodies against canine distemper virus by a new recombinant nucleocapsid protein-based enzyme-linked immunosorbent assay. Journal of Clinical Microbiology, 37(4), 1049–1056.
  • Wang, J., Li, R., Shi, R., Liu, L., & Yuan, W. (2018). Evaluation of an incubation instrument-free reverse transcription recombinase polymerase amplification assay for rapid and point-of-need detection of canine distemper virus. Journal of Virological Methods 260, 56–61.
  • Zarnke, R.L., Ver Hoef, J.M., & DeLong, R.A. (2004). Serologic survey for selected disease agents in wolves (Canis lupus) from Alaska and the Yukon Territory 1984-2000. The Journal of Wildlife Diseases, 40, 632-638.
Year 2021, , 45 - 51, 24.12.2021
https://doi.org/10.53913/aduveterinary.1000408

Abstract

Project Number

VTF-18020

References

  • Anis, E., Holford, A.L., Galyon G.D., & Wilkes R.P. (2018). Antigenic analysis of genetic variants of Canine distemper virus. Veterinary Microbiology, 219, Pages 154-160.
  • Appel, M.J.G., & Summers, B.A., (1999). Canine distemper: Current Status. Recent Advances in Canine Infectious Diseases. Erişim:[ http://www.ivis.com.]
  • Barrett, T. (1999). Morbillivirus infections with special emphassis on morbilliviruses of carnivores. Veterinary Microbiology, 69, 3-13.
  • Beineke, A, Puff, C, Seehusen, F, & Baumgärtner, W. (2009). Pathogenesis and immunopathology of systemic and nervous canine distemper, Veterinary Immunology and Immunopathology, 127, 1-18.
  • Blixenkrone-Møller, M., Svansson, V., Have, P., Orvell, C., Appel, M., Rode Pedersen, I., & Henriksen, P. (1993). Studies on manifestations of canine distemper virus infection in an urban dog population. Veterinary Microbiology, 37(1-2), 163–173.
  • Budaszewski R. (2016). Influence of vaccine strains on the evolution of canine distemper, Infection, Genetics and Evolution, 41, 262-269.
  • Çalışkan E, & Burgu I. Köpek gençlik hastalığı virusunun prevalansı ve seroepidemiyolojisi. Veterinary Microbiology 2007, 18, 5-10.
  • Çalışkan E. (2007). Köpek Gençlik Hastalığı Virus İzolasyonu, H Proteini kodlayan gen bölgesinin karakterizasyonu ve seroepidemiyolojisi, Doktora Tezi, Ankara Üniversitesi, Sağlık Bilimleri Enstitüsü, Ankara.
  • Çalışkan, E, & Burgu, I. (2007). Köpek gençlik hastalığı virusunun prevalansı ve seroepidemiyolojisi. Veterinary Microbiology, 18, 5-10.
  • Damián, M., Morales, E., Salas, G., & Trigo, F. J. (2005). Immunohistochemical Detection of Antigens of Distemper, Adenovirus and Parainfluenza Viruses in Domestic Dogs with Pneumonia. Journal of Comparative Pathology 133(4), 289–293.
  • Dorji, T., Tenzin, T., K. Tenzin, K., Tshering, D., Rinzin, K., Phimpraphai, W., & de Garine-Wichatitsky, M (2020). Seroprevalence and risk factors of canine distemper virus in the pet and stray dogs in Haa, western Bhutan. BMC Veterinary Research, 16, 135. https://doi.org/10.1186/s12917-020-02355-x.
  • Elia, G., Camero, M., Losurdo, M., Lucente, M. S., Larocca, V., Martella, V., Decaro, N., & Buonavoglia, C. (2015). Virological and serological findings in dogs with naturally occurring distemper. Journal of Virological Methods. 213, 127-130.
  • Esin, E. (2013). Köpeklerde Canine Distemper Virus Enfeksiyonun Araştırılması, Doktora Tezi, Konya Selçuk Üniversitesi, Sağlık Bilimleri Enstitüsü, Konya.
  • ICTV (2020). International Committee on Taxonomy of Viruses. https://talk.ictvonline.org/taxonomy/ (accessed 20 August 2021).
  • Koutinas, A.F., Baumgärtner, W., Tontis, D., Polizopoulou, Z., Saridomichelakis, M.N., & Lekkas, S. (2004). Histopathology and immunohistochemistry of canine distemper virus-induced footpad hyperkeratosis (hard Pad disease) in dogs with natural canine distemper. Veterinary Pathology, 41, 2-9.
  • Latha D, Geetha M, Ramadass, P., & Narayanan, R.B. (2007). Development of recombinant nucleocapsid protein based IgM-ELISA for the early detection of distemper infection in dogs. Veterinary Immunology and Immunopathology, 119, 278-286.
  • Latha, D., Srinivasan, S.R., Thirunavukkarasu, P.S., Gunaselan, L., Ramadass, P., Narayanan, R.B. (2007). Assessment of canine distemper virus infection in vaccinated and unvaccinated dogs. Indian Journal of Biotechnology, 6, 35-40.
  • Nguyen Thi, The Growth Profiles of Three Types of Canine Distemper Virus on Vero Cells, The Journal of Veterinary Medical Science 2005,67(5):491-495.
  • Panzera, Y., Sarute, N., Iraola, G., Hernández, M., & Pérez, R. (2015). Molecular phylogeography of canine distemper virus: Geographic origin and global spreading. Molecular Phylogenetics and Evolution, 6-15.
  • Pardo, M.C., Tanner, P., Bauman, J., Silver, K., & Fischer, L. (2007). Immunization of puppies in the presence of maternally derived antibodies against canine distemper virus. Journal of Comparative Pathology, 137, 72-75.
  • Richmann, D.D., Whitley R.J., & Hayden, F.G. (2009). Clinical virology, 4th edition, American Society for Microbiology. Washington, 1489.
  • Rikula UK. Canine distemper in Finland-vaccination and epidemiology, Academic Disertation, University of Helsinki, Finland, 2008.
  • Sabatino, D. (2016). Lethal distemper in badgers (Meles meles) following epidemic in dogs and wolves, Infection, Genetics and Evolution, 46,130-137.
  • Saltık H.S.(2018). Klinik semptomlu köpeklerin kan, idrar, rektal sürüntü, nasal ve oküler akıntı örneklerinde canine distemper virus enfeksiyonun real time RT-PCR ile tespit edilmesi. Doktora Tezi, Mehmet Akif Ersoy Üniversitesi, Sağlık Bilimleri Enstitüsü, Burdur.
  • Saltık, H.S., Kale, M. (2020). Evaluation of infection with N protein-specific Immunoglobulin M and G in naturally occurring distemper in dogs. Veterinarni Medicina, 65, 2020 (04), 168–173. https://doi.org/10.17221/31/2019-VETMED.
  • Sugai A, Kooriyama T, Sato H, Yoneda M, & Kai C. (2009). Epitope mapping of Canine distemper virus phosphoprotein by monoclonal antibodies. Microbiology and Immunology., 53, 667-674.
  • Şahna KC, Gençay A, & Atalay O. Viral etilogy of diarrhoea in puppies from a same shelter in turkey, presence of mixed infections. Revue de Médecine Vétérinaire 2008, 159, 345-347.
  • Terio, K.A., & Craft ME. Canine distemper virus (CDV) in another big cat, should CDV be renamed carnivore distemper virus? Microbiology. 2013, 4, 702-713.
  • Vivaldo, G. (2019). Molecular and serological surveys of canine distemper virus: A meta-analysis of cross-sectional studies, PLoS One. 14. https://doi.org/10.1371/journal.pone.0217594.
  • Von Messling, V., Harder T.C., Moennig, V., Rautenberg, P., Nolte, I., & Haas, L. (1999). Rapid and sensitive detection of ımmunoglobulin M (IgM) and IgG antibodies against canine distemper virus by a new recombinant nucleocapsid protein-based enzyme-linked immunosorbent assay. Journal of Clinical Microbiology, 37(4), 1049–1056.
  • Wang, J., Li, R., Shi, R., Liu, L., & Yuan, W. (2018). Evaluation of an incubation instrument-free reverse transcription recombinase polymerase amplification assay for rapid and point-of-need detection of canine distemper virus. Journal of Virological Methods 260, 56–61.
  • Zarnke, R.L., Ver Hoef, J.M., & DeLong, R.A. (2004). Serologic survey for selected disease agents in wolves (Canis lupus) from Alaska and the Yukon Territory 1984-2000. The Journal of Wildlife Diseases, 40, 632-638.
There are 32 citations in total.

Details

Primary Language English
Subjects Veterinary Sciences
Journal Section Research Articles
Authors

Yusuf Sayın

Nural Erol

Project Number VTF-18020
Publication Date December 24, 2021
Submission Date September 24, 2021
Published in Issue Year 2021

Cite

APA Sayın, Y., & Erol, N. (2021). Investigation of Canine Distemper Virus Infection in Dogs in the Antalya Province. Animal Health Production and Hygiene, 10(2), 45-51. https://doi.org/10.53913/aduveterinary.1000408