Isolation and Antimicrobial Susceptibility of Some Bacteria From The Gut of Honey Bees in Siirt Province of Türkiye

Abstract

Bu çalışmada, Siirt ili ve yöresinde bulunan bal arılarının bağırsak içeriklerinden bazı aerobik bakterilerin varlığı araştırıldı. Bakteriyel etkenler konvansiyonel bakteriyolojik yöntemlerle izole edildi ve ticari identifikasyon test kiti ile identifiye edildi. İzolatların antimikrobiyal duyarlılığı disk difüzyon testi ile belirlendi. Çalışmada en yüksek oranda izole edilen etkenlerin Staphylococcus spp. ve Klebsiella spp. olduğu ve bunu sırasıyla Bacillus spp. Izolatlarının izlediği belirlendi. GSBL ve plasmidik AmpC direnci 12 adet Gram negatif etkenin 6 (%50)’sında tespit edildi. Ayrıca Enterobacteriaceae izolatlarında imipenem direncinin yüksek olduğu belirlendi. Buna karşın Staphylococcus spp. izolatlarının çalışmada kullanılan antimikrobiyal maddelerin çoğuna duyarlı olduğu görüldü. Çalışmadan elde edilen verilerin bal arıları ile ilgili yapılan çalışmalara katkı sağlayacağı düşünüldü.

Keywords

• honey bee
• bacteria
• antimicrobial susceptibility

Siirt İli ve Yöresindeki Bal Arılarının Bağırsak İçeriklerinden Bazı Bakteriyel Etkenlerin İzolasyonu ve Antimikrobiyal Duyarlılıkları

Abstract

Bu çalışmada, Siirt ili ve yöresinde bulunan bal arılarının bağırsak içeriklerinden bazı aerobik bakterilerin varlığı araştırıldı. Bakteriyel etkenler konvansiyonel bakteriyolojik yöntemlerle izole edildi ve ticari identifikasyon test kiti ile identifiye edildi. İzolatların antimikrobiyal duyarlılığı disk difüzyon testi ile belirlendi. Çalışmada en yüksek oranda izole edilen etkenlerin Staphylococcus spp. ve Klebsiella spp. olduğu ve bunu sırasıyla Bacillus spp. Izolatlarının izlediği belirlendi. GSBL ve plasmidik AmpC direnci 12 adet Gram negatif etkenin 6 (%50)’sında tespit edildi. Ayrıca Enterobacteriaceae izolatlarında imipenem direncinin yüksek olduğu belirlendi. Buna karşın Staphylococcus spp. izolatlarının çalışmada kullanılan antimikrobiyal maddelerin çoğuna duyarlı olduğu görüldü. Çalışmadan elde edilen verilerin bal arıları ile ilgili yapılan çalışmalara katkı sağlayacağı düşünüldü.

Keywords

• Bal arısı
• bakteri
• antimikrobiyal duyarlılık

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