Molecular Typing of Vibrio species Isolated from Seabass (Dicentrarchus labrax) and Detection of Antibiotic Resistance

Year 2024, , 9 - 20, 30.12.2024

Oğuzhan Dolgun , Şükrü Kırkan , Hafize Tuğba Yüksel Dolgun

https://doi.org/10.53913/aduveterinary.1528574

Abstract

This study was conducted to isolate Vibrio species from sea bass, identify them using molecular methods, and determine their antibiotic resistance. In the research, 100 sea bass samples taken from fish farms in the Aegean region between May and September 2021 were examined. After the phenotypic and genotypic identification of the isolates obtained from the samples, antibiotic resistances were determined by the disk diffusion method and antibiotic resistance genes were determined by multiplex PCR. In this study, 46 (46%) Vibrio spp. isolates were obtained from 100 sea bass samples by conventional and biochemical methods. The obtained 46 isolates were confirmed to be Vibrio spp. by 16S rRNA PCR. From 46 isolates, 22 (47,8%) isolates were identified as V. alginolyticus, 13 (28,2%) isolates as V. harveyi, 3 (6,5%) isolates as V. parahaemolyticus, 1 (2%) isolate as V. vulnificus and 7 (15%) isolates as Vibrio spp. by multiplex PCR. The highest resistance was found to ampicillin (84,8%) in the isolates in the antibiogram. All isolates were found to be susceptible to enrofloxacin and sulfamethoxazole-trimethoprim (100%). In isolates, the highest resistance gene was found to be trimethoprim resistance gene (63%), and the lowest resistance gene was found to be fluoroquinolone resistance gene (6,5%). In this study, it was determined that Vibrio species have an important role as a primary agent in fish diseases, molecular methods give more reliable results in identification, and there is single and multiple antibiotic resistance among isolates.

Keywords

Antibiotic resistance, identification, PCR, Vibrio spp.

Supporting Institution

Aydın Adnan Menderes University

Project Number

ADÜ- BAP (VTF-19003).

Thanks

This study was supported by Aydın Adnan Menderes University Scientific Research Projects (VTF-19003).

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