Determination of Thiol/Disulfide Homeostasis and Oxidative Stress Index in Sheeppox Virus

Year 2024, , 21 - 25, 30.12.2024

Sinan Adıgüzel , Oğuz Merhan

https://doi.org/10.53913/aduveterinary.1534512

Abstract

The aim of the study was to determine the thiol/disulfide homeostasis and oxidative stress parameter levels in sheep infected with pox virus and to investigate their diagnostic significance. 1-2 years old Morkaraman breed sheep (20 infected with pox virus, 10 healthy) were used in the study. Total thiol, native thiol, total antioxidant capacity (TAC) and total oxidant capacity (TOC) analyses were performed in sera obtained from blood samples taken from the jugular vein of the sheep in tubes without anticoagulant. When the pox virus infected group and healthy sheep were compared, it was determined that total thiol and native thiol levels were significantly decreased; disulfide, disulfide/native thiol and disulfide/total thiol levels increased but were not statistically significant. In addition, it was determined that serum TOC and oxidative stress index (OSI) levels increased, while TAC level decreased. In conclusion, the findings obtained in the study suggest that the disease causes oxidative stress, and the use of oxidative stress markers, especially thiol/disulfide homeostasis markers, may contribute to the pathogenesis of smallpox and be an auxiliary parameter for its diagnosis.

Keywords

Oxidative stress index, sheeppox, thiol/disulfide homeostasis

References

• Akaike, T. (2001). Role of free radicals in viral pathogenesis and mutation. Reviews in Medical Virology, 11, 87-101. https://doi.org/10.1002/rmv.303
• Aksoy, M., & Kirmit, A. (2020). Thiol/disulphide balance in patients with psoriasis. Advances in Dermatology and Allergology, 37(1), 52-55. https://doi.org/10.5114/ada.2018.77767
• Ates, I., Ozkayar, N., Inan, B., Yilmaz, F. M., Topcuoglu, C., Neselioglu, S., Erel, O., Dede, F., & Yilmaz, N. (2016). Dynamic thiol/ disulphide homeostasis in patients with newly diagnosed primary hypertension. Journal of the American Society of Hypertension: JASH, 10(2), 159-166. https://doi.org/10.1016/j.jash.2015.12.008
• Aydın, O., Özkurt, G., Çamkerken, İ., Eren, E., Yanar, K. E., & Aktaş, M. S. (2023). Investigation of ischemia-modified albumin and thiol/ disulfide homeostasis for the determination of oxidative stress in sheep with toxoplasmosis. Small Ruminant Research, 225, 107023. https://doi.org/10.1016/j.smallrumres.2023.107023
• Bhanuprakash, V., Indrani, B.K., Hosamani, M., & Singh, R.K. (2006). The current status of sheep pox disease. Comparative Immunology, Microbiology&Infectious Diseases, 29(1), 27-60. https://doi.org/10.1016/j.cimid.2005.12.001
• Bowden, T.R., Babiuk, S.L., Parkyn, G.R., Copps, J.S., & Boyle, D.B. (2008). Capripoxvirus tissue tropism and shedding: a quantitative study in experimentally infected sheep and goats. Virology, 371, 380-393. https://doi.org/10.1016/j.virol.2007.10.002
• Bozukluhan, K., Merhan, O., Özcan, A., Gökçe, H.İ., & Gökçe, G. (2017). Toxocara vitulorum ile doğal enfekte buzağılarda serum haptoglobin düzeyi, oksidatif belirteçler ve bazı biyokimyasal parametrelerin düzeyinin belirlenmesi. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 64(2), 75-79. https://doi.org/10.1501/Vetfak_0000002778
• Çamkerten, İ., Çamkerten, G., Erdoğan, H., Ayan, A., Erdoğan, S., & Ural, K. (2019). Serum thiol disulphide levels among sheep with sarcoptic mange. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 25(6), 865- 868. https://doi.org/10.9775/kvfd.2019.22083
• Değirmençay, Ş., Çamkerten, G., Çamkerten, İ., & Aktaş, M. S. (2021). An investigation of thiol/disulfide homeostasis and ischemia-modified albümin levels to assess the oxidative stress in dogs with canine distemper. Veterinarski Arhiv, 91(1), 39-49. https://doi.org/10.24099/vet.arhiv.0867
• Deveci, H.A., Kükürt, A., Uzlu, E., Sözdutmaz, İ., Merhan, O., Aktaş, S., Alpay, M., Kaya, İ., & Karapehlivan, M. (2017). Evaluation of paraoxonase activity, total sialic acid and oxidative stress in sheep with ecthyma contagiosa. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 23(3), 453-457. https://doi.org/10.9775/kvfd.2016.17001
• Deveci, M. Z. Y., & Erdal, H. (2022). Determination of dynamic thioldisulfide levels in dairy cattle with foot disease. Veterinarski Arhiv, 92(6), 657-666. https:/dx.doi.org/10.24099/vet.arhiv.1785
• El Mandrawy, S.A.M., & Alam, R.T.M. (2018). Hematological, biochemical and oxidative stress studies of lumpy skin disease virus infection in cattle. Journal of Applied Animal Research, 46(1), 1073- 1077. https://doi.org/10.1080/09712119.2018.1461629
• Emre, B., Korkmaz, Ö., Koyuncu, I., Çomaklı, S, Akçay, A, Zonturlu, A. K., & Erel, Ö. (2021). Determination of thiol/disulphide homeostasis as a new indicator of oxidative stress in dairy cows with subclinical endometritis. Veterinarski Arhiv, 91(2), 137-148. https://doi. org/10.24099/vet.arhiv.0914
• Erdoğan, H., Çamkerten, İ., Çamkerten, G., Ural, K., Erdoğan, S., Günal, İ., & Erel, Ö. (2019). The effect of hot-iron disbudding on thiol-disulphide homeostasis in calves. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 25(3), 335-339. https://doi.org/10.9775/kvfd.2018.20950
• Erel, O (2005). A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry, 38(12), 1103-1111. https://doi.org/10.1016/j.clinbiochem.2005.08.008
• Erel, O. (2004). A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clinical Biochemistry, 37(4), 277-285. https://doi.org/10.1016/j.clinbiochem.2003.11.015
• Erel, O., & Neselioglu, S. (2014). A novel and automated assay for thiol/ disulphide homeostasis. Clinical Biochemistry, 47(18), 326-332. https://doi.org/10.1016/j.clinbiochem. 2014.09.026
• Ertaş, F., Kızıltepe, Ş., & Merhan, O. (2023). Investigation of dynamic thiol disulfide homeostasis in young cattle with pneumonia. MAS Journal of Applied Sciences 8(Special Issue), 949-954. https://doi.org/10.5281/zenodo.10003819
• Issi, M., Gul, Y., & Yilmaz, S. (2008). Clinical, haematological and antioxidant status in naturally poxvirus infected sheep. Revue de Medecine Veterinaire, 159(1), 54-58.
• Karababa, F., Yesilova, Y., Turan, E., Selek, S., Altun, H., & Selek, S. (2013). Impact of depressive symptoms on oxidative stress in patients with psoriasis. Redox Report, 18(2), 51-55. https://doi.org/10.1179/1351000213Y.0000000039
• Kirmizigul, A.H., Ogun, M., Ozen, H., Erkilic, E.E., Gokce, E., Karaman, M., & Kukurt, A. (2016). Oxidative stress and total sialic acid levels in sheep naturally infected with pox virus. Pakistan Veterinary Journal, 36(3), 312-315.
• Kükürt, A., Gelen, V., Başer, Ö. F., Deveci, A. H., & Karapehlivan, M. (2021). Thiols: role in oxidative stress-related disorders. In: P. Atukeren (Ed), Accenting Lipid Peroxidation (pp. 27-47). Intech Open. http://dx.doi.org/10.5772/intechopen.96682
• Merhan, O., Bozukluhan, K., & Gokce, H.I. (2017). Acute phase proteins and biochemical and oxidative stress parameters in Hypoderma spp. infested cattle. Journal of the Hellenic Veterinary Medical Society, 68(4), 535-540. https://doi.org/10.12681/jhvms.16049
• Merhan, O., Taşçı, G.T., Bozukluhan, K., & Aydın, N. (2020). Determination of oxidative stress index and total sialic acid in cattle infested with Hypoderma spp. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 26(5), 633-636. https://doi.org/10.9775/kvfd.2020.24071
• Özcan, O., Erdal, H., Çakırca, G., & Yönden, Z. (2015). Oksidatif stres ve hücre içi lipit, protein ve DNA yapıları üzerine etkileri. Journal of Clinical and Experimental Investigations, 6(3), 331-336. https://doi.org/10.5799/ahinjs.01.2015.03.0545
• Pizzino, G., Irrera, N., Cucinotta, M., Pallio, G., Mannino, F., Arcoraci, V., Squadrito, F., Altavilla, D., & Bitto, A. (2017). Oxidative stress: harms and benefits for human health. Oxidative Medicine and Cellular Longevity, 2017, 8416763. https://doi.org/10.1155/2017/8416763
• Plowright, W., MacLeod, W.G., & Ferris, R.D. (2012). The pathogenesis of sheep pox in the skin of sheep. Journal of Comparative Pathology, 146(2-3), 97-105. https://doi.org/10.1016/j.jcpa.2011.12.004
• Schmidt, E. M. D. S., Fachiolli, D. F., de Oliveira, R. M., Almeida, F. A., Pariz, C. M., de Lima Meirelles, P. R., Costa, C., Tvarijonaviciute, A., Erel, O., Neselioglu, S., Ceron, J. J., & Rubio, C. P. (2021). Changes in serum thiol-disulphide homeostasis in sheep with gastrointestinal nematodes. Animals: an Open Access Journal from MDPI, 11(10), 2856. https://doi.org/10.3390/ani11102856
• Tarhan, M., Deger, Y., Oguz, B., & Ozdek., U. (2023). Investigation of thiol-disulphide homeostasis, total oxidant-antioxidant and ischaemia-modified albumin levels in dogs with babesiosis. The Alexandria Journal of Veterinary Sciences, 77(1), 21-29. https://doi.org/10.5455/ajvs.145761
• Terzi, O. S., Kara, E., Şenel, Y., Ceylan, E., Neşelioğlu, S., & Erel, Ö. (2023). Dynamic thiol-disulphide homeostasis and ischemia modified albumin levels in neonatal calf diarrhea. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 70(1), 81-86. https://doi.org/10.33988/auvfd.928731