Comparative Macroscopic and Morphometric Analysis of Mandible in Different Mouse (Balb-c; Cd-1; C57bl/6) and Rat Strains (Wistar Albino; Sprague Dawley; Wag/Rij)

Abstract

Since the morphology of the mandible varies among animal species, it is the subject of many studies. The aim of this study was to investigate the macroanatomical and morphometric aspects of the mandible in three different rat (Spague Dawley, Wistar Albino, Wig/Rij) and mouse strains (Balb-c, C57bl/6, CD-1). In this study, which was conducted using a total of 96 mandibles, 8 females and 8 males from each species, measurements were taken from 6 parameters. Similarities and differences between strains and sexes were revealed by statistical analyses. The mandibles were examined under a stereomicroscope. For the Balb-c strain, the termination of the process of the angularis at the level of the process of the condylaris and the shallowness of the masseteric tubercle; for Wig/Rij rats, the proc. angularis, proc. The termination of the condylaris level, the prominent masseteric tubercle and the localization of the molar teeth were macroscopically revealed as distinctive anatomical structures. Specific measurement parameters were proc. coronoideus height for Balb-c mouse; mandible length and lower M3 crown length for CD-1 mouse, while no specific measurement point was available for C57bl/6 mouse. In rats, proc. coronoideus height was the specific measurement point for Wig/Rij rat; lower M3 crown length was decisive for Sprague Dawley. Mandible length parameter was specific for all rat strains. It was concluded that strain determination could be made by looking at these distinctive parameters.

Keywords

• anatomy
• mandible
• morphometry
• statistics

References

1. Ağaç, D.K., Oktay, E., Onuk, B., Kabak, M. & Gündemir, O. (2024a). Shape variation in cranium, mandible and teeth in selected Mouse strains. Anatomia Histologia Embryologia, 53(4), 1-11. https://doi.org/10.1111/ahe.13064
2. Ağaç, D.K., Onuk, B., Gündemir, O., Kabak, M., Manuta, N., Çakar, B. & Szara, T. (2024b). Comparative cranial geometric morphometrics among Wistar Albino, Sprague Dawley, and WAG/Rij rat strains. Animals, 14(9), 1274. https://doi.org/10.3390/ani14091274
3. Akbulut, Y., Demiraslan, Y., Gürbüz, İ. & Aslan, K. (2014). Yeni Zelanda tavşanı (Oryctolagus cuniculus L.)’nda cinsiyet faktörünün mandibula morfometrisine etkisi. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, 28(1), 15-18.
4. Aspinall, V. & O’Reilly, M. (2004). Introduction to Veterinary Anatomy and Physiology (First ed.). Philadelphia: Butterworth-Heinemann.
5. Bahadır, A. & Yıldız, H. (2018). Veteriner Anatomi Hareket Sistemi & İç Organlar (8th ed.) Bursa: Ezgi Kitabevi.
6. Bodner, L., Gabor, D. & Kaffe, I. (1998). Characteristics of the aging rat mandible. Archives of Gerontology and Geriatrics, 27(2), 147-157. https://doi.org/10.1016/S0167-4943(98)00108-3
7. Boell, L. & Tautz, D. (2011). Micro-evolutionary divergence patterns of mandible shapes in wild house mouse (Mus musculus) populations. BMC Evolutionary Biology, 11, 306.
8. Bookstein, F.L. (1991). Morphometric Tools For Landmark Data: Geometry And Biology. (first ed.). Cambridge University Press.

9. Brenner, S. Z., Hawkins, M. G., Tell, L. A., Hornof, W. J., Plopper, C. G. & Verstraete, F. J. (2005). Clinical anatomy, radiography, and computed tomography of the chinchilla skull. Compendium, 27(5), 933-944.
10. Çalışlar, T. (1978). Laboratuvar Hayvanları Anatomisi (14th ed.). Ankara Üniversitesi Basımevi.
11. Demiraslan, Y. & Dayan, O. (2021). Veteriner Sistemik Anatomi (2th ed.). Atlas Kitabevi.
12. Demirtaş, S., Özmen, M., Silsüpür, M. & Kıral, D. (2023). Comparative skull and mandible geometric morphometrics of two species of mice, Mus domesticus and Mus macedonicus (muridae, rodentia) in Turkey. Cumhuriyet Science Journal, 44(3), 444-449. https://doi:doi.org/10.17776/csj.1250269
13. Dikmen, S., Petek, M., Oğan, M. & Onbaşılar, E. (2011). Laboratuvar Hayvanları Yetiştirme ve Sağlığı (2th ed.). Anadolu Üniversitesi Yayını.
14. Dinç, G. (2001). Porsuk (Meles meles) detaylı sistemi üzerinde makroanatomik inceleme III. İskelet Axiale. Fırat Üniversitesi Sağlık Bilimleri Dergisi, 15(1), 175 – 178.
15. Dursun, N. (2008). Veteriner Anatomi I (12th ed.). Medisan Yayınevi.
16. Dyce, K.M., Sack, W.O. & Wensing, C.J. (2018). Veteriner Anatomi Konu Anlatımı ve Atlas (4th ed.). Güneş Tıp Kitabevleri.
17. Ellerman, J.R. (1948). Key to the rodents of South-West Asia in the British museum collection. Proceedings of the Zoological Society of London, 118, 765–817. https://doi.org/10.1111/j.1096-3642.1948.tb00406.x
18. Ellerman, J.R. & Morrison-Scott, T.C.S. (1951). Checklist of Palaearctic and Indian mammals 1758-1946 (2th ed.). British Museum (Natural History).
19. Enotomo, A., Watahiki, J., Yamaguchi, T., Irie, T., Tachikawa, T. & Maki, K. (2010). Effects of mastication on mandibular growth evaluated by microcomputed tomography. The European Journal of Orthodontics Advance Access, 32, 66-70. https://doi.org/10.1093/ejo/cjp060
20. Fujita, T. (2004). Effects of sex hormone disturbances on craniofacial growth in new born mice. Journal of Dental Research, 83(3), 250–254. https://doi.org/10.1177/154405910408300313
21. Gürbüz, İ., Demiraslan, Y., Gülbaz, F. & Aslan, K. (2016). Malakan atı mandibulasının cinsiyete göre morfometrik özellikleri. Eurasian Journal of Veterinary Sciences, 32(3), 136-140. https://10.15312/EurasianJVetSci.2016318390
22. Hidaka, S., Matsumoto, M., Hiji, H., Ohsako, S. & Nishinakagawa, H. (1998). Morphology and morphometry of skulls of raccoon dogs, Nyctereutes procyonoides and badgers, Meles meles. Journal of Veterinary Medical Science, 60(2), 161-167. https://doi.org/10.1292/jvms.60.161
23. İlgün, R. & Özüdoğru, Z. (2020). Macroanatomical and morphometric investigation of mandibula in Aksaray Malaklı dogs. Van Veterinary Journal, 31(1), 7-11. https://doi.org/10.36483/vanvetj.540404
24. İnce, G.N. & Pazvant, G. (2010). Ratlarda (Wistar Albino) Mandibula’nın morfometrisi. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, 36(1), 51-56.
25. Ketani, Ş., Kilinç, M., Erdoğan, S., Kaya, A. & Coşkun, Y. (2017). A macroanatomical investigation of the some skull Bonesof Nehring’s blind mole rats (Spalacidae: Nannospalaxnehringi). Anaomia Histologia Embryologia Journal of Veterinary Medicine, 46(3), 232-239. https://doi.org/10.1111/ahe.12262
26. König, H. E. & Liebich, H. G. (2018). Veteriner Anatomi (Evcil Memeli Hayvanlar) (6th ed.). Medipres Matbaacılık.
27. Luca L., Roberto D., Francesca S.M., Francesca P. (2003). Consistency of diet and its effects on mandibular morphogenesis in the young rat. Progress in Orthodontics, 4(1), 3-7. https://doi.org/10.1034/j.1600-9975.2002.02033.x
28. McFadden L.R., McFadden K.D. & Precious D.S. (1986). Effect of controlled dietary consistency and cage environment on the rat mandibular growth. The Anatomical Record, 215(4), 390-396. https://doi.org/10.1002/ar.1092150409
29. Missagia, R. & Perini, F. (2018). Skull morphology of the Brazilian shrew mouse Blarinomys breviceps (Akodontini; Sigmodontinae), with comparative notes on Akodontini rodents. Zoologischer Anzeiger, 277, 148-161. https://doi.org/10.1016/j.jcz.2018.09.005
30. Odman, A., Mavropoulas, A. & Kiliaridis, S. (2008). Do masticatory functional changes influence the mandibular morphology in adult rats. Archives of Oral Biology, 53(12), 1149-1154. https://doi.org/10.1016/j.archoralbio.2008.07.004
31. Özkan, Z.E. (2007). Macro-anatomical investigations on the skeletons of mole-rat (Spalax leucodon Nordmann) III. Skeleton axiale. Veterinarski Arhiv, 77(3), 281-289.
32. Rohlf, F.J. & Marcus, L.F. (1993). A revolution in morphometrics. Trends in Ecology & Evolution, 8(4), 129-132. https://doi.org/10.1016/0169-5347(93)90024-J
33. Şeker, P. S. (2009). Doğu Karadeniz Bölgesinde Yayılış Gösteren Pitymys Mcmurtrie, 1831 Alt Cinsinin (Mammalia: Rodentia) Morfolojik Analizi [Yükeklisans Tezi, Ankara Üniversitesi]. Fen Bilimleri Enstitüsü.https://tez.yok.gov.tr/UlusalTezMerkezi/tezSorguSonucYeni.jsp
34. Treuting, P. M., Dintzis, S. M. & Montine, K. S. (2017). Comparative anatomy and histology: A mouse, rat, and human atlas (2nd ed.). Academic Press.
35. Yalçın, H. & Arslan, A. (2009). Ağaç ve yer sincaplarının (Rodentia: Sciuridae) kafa kemikleri üzerinde karşılaştırmalı morfolojik bir araştırma. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 4(2), 87-95.
36. Yalçın, H., Kayış, S.A. & Arslan, A. (2007). A comparative macroanatomic, mechanical and geometric morphometrics study on tree and ground squirrel and rat. Veteriner Bilimleri Dergisi, 23(1), 83-95.
37. Yılmaz, S. (1998). Macro-anatomical investigations on the skeletons of porcupine (Hystrix cristata). Part III: Skeleton axiale. Anatomia Histologia Embriyologia, 27, 293–296. https://doi.org/10.1111/j.1439-0264.1998.tb00196.x
38. Yılmaz, S., Dinç, G. & Toprak, B. (2000). Macro-anatomical investigations on skeletons of otter (Lutra lutra). III. Skeleton axiale. Veterinarski Arhiv. 70(4), 191-198. https://hrcak.srce.hr/100514
39. Zarei, B., Darvish, J., Aliabadian, M. & Moghaddan, F. (2013). Geometric morphometric analyses of four species of brush-tailed mice, genus Calomyscus (Rodentia: Calomyscidae), from the Iranian Plateau. Iranian Journal of Animal Biosystematics, 9(1), 73-81. https://doi.org/10.22067/IJAB.V911.33310