Farklı Çözücülerde Ekstrakte Edilen Propolisin Mide Koruyucu Etkinliğinin Araştırılması

Abstract

Propolis bal arılarının bitkilerin reçinelerini toplayarak oluşturdukları bir üründür. Bitkilerin reçinesinde bulunan ve propolise geçen içerisinde fenolik bileşenlerinde bulunduğu sekonder metabolitler propolise birçok biyolojik özellik kazandırır. Propolisin antibakteriyel, antiviral, antifungal, antioksidan, immunmodülatör, antiinflamatuvar, antiülser ve yara iyileşmesini hızlandırıcı gibi birçok özelliği bulunmaktadır. Bu özellikler çevredeki bitki örtüsü, mevsim, yükseklik, iklim kuşağı ve ekstraksiyon çözücüsüne göre değişkenlik göstermektedir. Bu çalışmada, hidro-alkolik, su, dimetilsülfoksit (DMSO) ve zeytinyağı kullanılarak hazırlanan farklı propolis ekstraktlarının her grupta 8 adet Balb/C fare olacak şekilde 9 grupta indometazin ile indüklenen gastrik ülser üzerindeki etkileri araştırılmış ve karşılaştırılmıştır. Epitel kaybı, erozyon, kanama, ödem, inflamatuar hücre infiltrasyonu ve ortalama klinik skor sonuçları, omeprazole kıyasla farklı propolis ekstraktları arasında istatistiksel olarak değerlendirildi. Histopatolojik sonuçlar birbiri ile benzer olduğu için genel değerlendirme ortalama klinik skoru ile yapılmıştır. DMSO ve alkol ekstrakt propolislerin omeprazole benzer gastroprotektif etkilerinin olduğu hidro-alkolik ve su ekstraktının ise kendi çözücülerine göre önemli fark oluşturmadığı ve gastroprotektif etkinlik göstermediği belirlenmiştir. Sonuç olarak, özellikle doğrudan tüketime uygun olan propolisin zeytinyağı ekstraktının gastroprotektif olarak kullanılabilme potansiyeli olduğu belirlenmiştir.

Keywords

• Apiterapi
• bal arısı
• DMSO
• zeytinyağı
• propolis
• mide koruyucu

Project Number

1919B012109713

Investigation of the Gastroprotective Activity of Propolis Extracted in Different Solvents

Abstract

Propolis is a product produced by honeybees that collects the resins of plants. Secondary metabolites, including phenolic compounds, which are found in the resin of plants and are incorporated into propolis, give propolis many biological properties. Propolis exhibits various properties such as antibacterial, antiviral, antifungal, antioxidant, immunomodulator, anti-inflammatory, antiulcer and wound healing accelerator. The properties vary depending on the source of the plant, season, altitude, climate zone and extraction solvent. The present study investigated and compared the effects of different propolis extracts prepared using alcohol, water, dimethyl sulfoxide (DMSO), and olive oil on the indomethacin-induced gastric ulcers in nine group of Balb/C mice (n=8). Epithelial loss, erosion, bleeding, edema, inflammatory cell infiltration, and mean clinical scores results were evaluated statistically between different propolis extracts compared to omeprazole. Since the histopathological results were remarkably similar, the general assessment was made with the mean clinical score. It was found that DMSO and olive oil extracts of propolis had gastroprotective effects similar to omeprazole. In contrast, hydro-alcohol and water extracts did not show significant differences compared to their solvents and gastroprotective activity. In conclusion, it was determined that the olive oil extract of propolis, which is especially suitable for direct consumption, has the potential to be used as a gastroprotective.

Keywords

• Apitherapy
• DMSO
• honeybee
• olive oil
• propolis
• gastroprotective

Supporting Institution

TUBİTAK 2209 A

Project Number

1919B012109713

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