Determination of AdipoR1 and AdipoR2 receptors gene expression metabolic syndrome occurs with fructose in the diet in rats

Abstract

In this study, it was aimed to investigate serum adiponectin levels and AdipoR1 and AdipoR2 gene expressions in rats with metabolic syndrome with fructose diet. Twenty-eight male Sprague Dawley rats were used for this purpose. The rats in the control group were fed only with water and rat food while the rats in the experimental group were fed with water containing 20% D-fructose and rat food for 16 weeks with ad libitum. In the control and metabolic syndrome groups, weight differences between the beginning and end of the experiment and the abdominal circumference measured at the end of the experiment were found statistically significant at the level of p <0.001. Adiponectin levels were measured by ELISA in serum samples taken at the end of the experiment. Fructose administration caused a statistically significant decrease in serum adiponectin levels compared to the control group (p <0.001). At the end of the period of fructose application time in liver tissue, quantitative change of AdipoR1 gene was observed as 1.97 fold decrease compared to control according to RT-PCR results, while a decrease of 3.11 fold was observed in the quantitative change of AdipoR2 gene. As a result, high fructose consumption decreases serum adiponectin levels and significantly deteriorates adiponectin receptor expression in the liver.

Keywords

• Metabolic syndrome
• Fructose
• adiponectin
• AdipoR1
• AdipoR2

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