Polymerase chain reaction PCR inhibitors

Abstract

Backround/Aim: Polymerase chain reaction PCR is a specific and sensitive molecular biological technique that has been used intensively in recent years in scientific studies. PCR is highly sensitive to inhibitors since it is an enzymatic reaction. The formation of these inhibitors is a significant disadvantage of PCR. Because the formation of inhibitors can affect the sensitivity of the results, as well as cause false negative results. The purpose of this review is to give information about the inhibitors that can be anticipated in the intended PCR studies and the precautions that should be taken to remove them.

Keywords

• Inhibitor,Molecular methods,PCR

Polimeraz Zincir reaksiyonu PZR İnhibitörleri

Abstract

Özbilgi/Amaç: Polimeraz zincir reaksiyonu PZR son yıllarda bilimsel çalışmalarda oldukça yoğun kullanılmaya başlanan özgül ve duyarlı bir moleküler biyolojik bir tekniktir. PZR, enzimatik bir reaksiyon olduğundan inhibitörlere karşı oldukça duyarlıdır. Bu inhibitörlerin oluşması PZR’ın önemli bir dezavantajıdır. Çünkü inhibitörlerin oluşması sonuçların duyarlılığını etkileyeceği gibi, yanlış negatif sonuçlara da neden olabilmektedir. Bu derleme’nin amacı PZR çalışmalarında karşımıza çıkabilecek inhibitörler ve bunların ortadan kaldırılmaları için alınması gereken önlemler hakkında bilgi verilmesidir.

Keywords

• İnhibitör,Moleküler yöntemler,PZR

References

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