The Effectiveness of Progesterone-Releasing Intravaginal Device with Daily GnRH Injections on the Reproductive Parameters of Mares in Diestrus

Abstract

This study examines the effectiveness of PRID solely and combined with GnRH treatment on fertility parameters of mares. At day seven after ovulation, PRID 1.55 g P4+10 mg E2-benzoate was inserted intravaginally for nine days in G1 n=8 and G2 n=7 . In the same period, G1-mares received GnRH 2.5ml/mare, Receptal® on a daily basis. At day 16 day of PRID removal PGF2a 500μg/mare, Estrumate® was administered to G1 and G2. Mares of G3 n=8 served as controls. Follicle controls were started at day 16 in G1 and G2, and when the mares showed positive response to stallions in G3. After day 16 in G1 and G2, mares that did not show signs of oestrus and ovulation within eight days were considered to be non-responder NR . Mating was performed every other day until ovulation occurred after determination of preovulatory follicle POF . NR mares were found to be 12.5% G1 and 14.3% G2 . The pregnancy rate was not statistically significant between the groups. Interval from PRID removal-ovulation was determined to be 6.4 days in G1 and 5.7 days in G2. Interval from POF-ovulation in G1 1.2 day was found to be significantly lower than in G2 3.2 day and G3 3.6 day P0.05 . The significance between P4 concentrations of G1 and G2 was not found throughout PRID treatment P>0.05 , except second day of PRID insertion P

Keywords

• Fertility,GnRH,Mare,PRID,Synchronization

Diöstrusteki Kısraklara Günlük GnRH Enjeksiyonuyla Birlikte Uygulanan Progesteron-Salgılayan İntravaginal Cihazın Reprodüktif Parametreler Üzerine Etkisi

Öz

Sunulan çalışmada kısraklarda tek başına ve günlük GnRH uygulamasıyla kombine kullanılan PRID’in fertilite parametreleri üzerindeki etkisi araştırıldı. G1 n=8 ve G2 n=7 ’de ovulasyondan yedi gün sonra PRID 1.55 g P4+10 mg E2-benzoat intravaginal olarak uygulandı ve dokuz gün süreyle tutuldu. Aynı zamanda G1’deki kısraklara GnRH 2.5ml/kısrak, Receptal® günlük uygulandı. G1 ve G2’ye PRID’in çıkartıldığı 16. günde PGF2a 500μg/kısrak, Estrumate® yapıldı. G3 n=8 kontrol grubu olarak kullanıldı. Follikül kontrollerine G1 ve G2’de 16.günde, G3’te ise kısraklar aygıra pozitif cevap verdiğinde başlanıldı. G1 ve G2‘de sekiz gün içinde östrus ve ovulasyon göstermeyen kısraklar tedaviye cevap vermedi NR olarak kabul edildi. Aşımlar preovulatorik follikül POF belirlendikten sonra ovulasyon şekilleninceye kadar gün aşırı olarak yaptırıldı. NR %12.5 G1 ve %14.3 G2 olarak belirlendi. Gebelik oranları arasında fark bulunmadı. PRID kaldırılması-ovulasyon aralığı G1’de 6.4 gün G2’de 5.7 gün olarak bulundu. POF-ovulasyon aralığı G1’de 1.2 günle G2 3.2 gün ve G3 3.6 gün ’den düşük bulundu P0.05 . İkinci gün dışında P0.05 . Sonuç olarak PRID’in tek başına veya günlük GnRH uygulamasıyla kombine olarak kullanılmasının diöstrus dönemindeki kısraklarda fertilite parametrelerini etkilemediği sonucuna varıldı.

Anahtar Kelimeler

• Fertilite,GnRH,Kısrak,PRID,Senkronizasyon

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