Isolation of Microorganisms from Goats with Subclinical Mastitis and Detection of Antibiotics Susceptibility

Abstract

In this study, microorganisms were isolated and identified to 102 67.1% samples from to 152 hair goat’s milk. There was not obtained any bacterial isolation from the remaining 50 32.9% samples. Our of identified 102 samples were identified respectively; S. aureus from 71 69.6% samples, S. epidermidis from 8 7.8% samples, S. intermedius from 5 4.9% samples, S. hyicus from 6 5.9% samples, Corynebacterium sp. from 3 2.9% samples, Klebsiella pneumoniae from 4 3.9% samples, Pseudomonas sp. from 2 2.0% samples, E. coli from 2 2.0% samples and Mannheimia haemolytica from 1 1.0% sample. As a result of antibiotic susceptibility tests, S. aureus isolates were found susceptible to Amoxycillin- Clavulanic Acid in the ratio of 100%, susceptible to Penicilin in the ratio of 100%, resistant to Kanamycin and Oxacillin ratio of 90%. The other Staphylocci isolates were found susceptible to Amoxycillin- Clavulanic Acid in the ratio of 100%, resistant to Penicilin ratio of 100%. Other identified isolates Corynebacterium sp., Klebsiella pneumoniae, Pseudomonas sp., E. coli and Mannheimia haemolytica were found susceptible to Ampicillin in the ratio of 100%, susceptible to Amoxycillin- Clavulanic Acid in the ratio of 90%, resistant to Penicilin and Kanamycin in the ratio of 100%.

Keywords

• Goat,Mastitis,Microorganism,Isolation,Identification

Subklinik Mastitisli Keçilerden Mikroorganizmaların İzolasyonu ve Antibiyotiklere Duyarlılıklarının Araştırılması

Öz

Araştırmada 152 adet kıl keçisi süt örneğinin 102 %67.1 ’sinden bakteri izolasyon ve identifikasyonu yapılmıştır. Geriye kalan 50 %32.9 örnekte ise bakteriyel üreme görülmemiştir. İdentifikasyon yapılan 102 örneğin, 71 %69.6 ’inden S. aureus, 8 %7.8 ’inden S. epidermidis, 5 %4.9 ’inden S. intermedius, 6 %.5.9 ’sından S. hyicus, 3 %2.9 ’ünden Corynebacterium sp., 4 % 3.9 ’ünden Klebsiella pneumoniae, 2 %2.0 ’sinden Pseudomonas sp., 2 % 2.0 ’sinden E. coli ve 1 %1.0 ’inden Mannheimia haemolytica identifiye edilmiştir. Araştırmada identifiye edilen suşlara yapılan antibiyogram testleri sonucunda S. aureus izolatlarının Amoksisilin-Klavulanik Asit’e %100 duyarlı, Penisilin’e % 100, Kanamisin ve Oksasilin’e ise %90 oranlarında dirençli olduğu tespit edilmiştir. Diğer izole edilen Stafilokok suşlarının da Amoxicillin- Clavulanic Acid’e %100, duyarlı, Penisilin’e %100 oranında dirençli olduğu tespit edilmiştir. İdentifiye edilen Corynebacterium sp., Klebsiella pneumoniae, Pseudomonas sp., E. coli ve Mannheimia haemolytica suşlarının antibiyogram testleri sonucunda Ampisilin’e % 100 ve Amoksisilin-Klavulanik Asit’e %90 oranlarında duyarlı; Penisilin ve Kanamisin’e %100 oranında dirençli oldukları tespit edilmiştir.

Anahtar Kelimeler

• Keçi,Mastitis,Mikroorganizma,İzolasyon,İdentifikasyon

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