Trehalose as a Cryoprotective Agent for the Sperm Cells: A Mini Review

Abstract

Although many protocols have been developed for semen cryopreservation, sperm cryosurvival rate is still not optimum in most of the species. Factors responsible for low cryosurvival rate are; changes in temperature, ice formation, lipid peroxidation, alterations in sperm membrane, toxicity of cryoprotectants and osmotic stress. Moreover, cell dehydration during freezing process is one of the factors altering the structural and functional integrity of the plasma membrane. Sugars have several functions in sperm extender, including providing energy substrate for the sperm cell during incubation, maintaining the osmotic pressure of the diluents, and acting as a cryoprotectant. Trehalose, α-D-glucopyranosyl-α-D-glucopyranoside a non-permeable sugar, has a significant role to prevent deleterious effect of cell dehydration on plasma membrane. Recent reports have shown encouraging data regarding the use of trehalose for the cryopreservation of sperm cells. Therefore, in this review, information is presented related to the use of trehalose for cryopreserving sperm from different species.

Keywords

• Cryoprotective agent,Sperm cell,Trehalose

Spermatozoon Dondurulmasında Koruyucu Bir Madde Trehalose: Kısa Derleme

Abstract

Spermanın dondurularak saklanmasına yönelik bir çok yöntemin geliştirilmiş olmasına rağmen, bir çok hayvan türünde çözüm sonu yaşama oranları henüz istenen düzeye ulaşmamıştır. Sıcaklık değişimleri, buz oluşumu, lipidoksidasyonları, membran yapısındaki değişiklikler, kriyoprotektanların toksik etkileri ve ozmotik stres bu düşük yaşama oranlarına yol açan temel faktörlerdir. Bunların yanı sıra dondurma sırasında hücrenin su kaybetmesi de plazma membranının yapısal ve fonksiyonel bütünlüğünü bozan bir faktördür. Sperma sulandırıcılarına eklenen şekerler spermatozoonlar için enerji kaynağı oluşturmanın ötesinde ozmotik basıncı ayarlar ve kriyoprotektan olarak etki gösterirler. Non-permeable bir şeker olan trehaloz α-D-glucopyranosyl-α-D-glucopyranoside su kaybı sırasında hücre membranında oluşan olumsuz etkilerin önlenmesinde önemli bir etkiye sahiptir. Son yıllarda yapılan çalışmalar spermanın dondurularak saklanması sırasında trehaloz kullanmayı teşvik eden bulgular sunmaktadır. Bu nedenle, bu derlemede çeşitli hayvan türlerinde sperma dondurma işlemleri sırasında trehaloz kullanımına ilişkin bilgiler sunulmuştur.

Keywords

• Kriyoprotektan maddeler,Spermatozoon,Trehaloz

References

1. Aboagla EME, Terada T (2003). Trehalose-enhanced fluidity of the goat sperm membrane and its protection during freezing. Biology of Reproduction, 69, 1245-1250.
2. Aisen E, Quintana M, Medina V, Morello H, Venturino A (2005). Ultramicroscopic and biochemical changes in ram spermatozoa cryopreserved with trehalose-based hypertonic extenders. Cryobiology, 50, 239-249.
3. Aisen EG, Alvarez HL, Venturino A, Garde JJ (2000).Effect of trehalose and EDTA on cryoprotective action of ram semen diluents. Theriogenology, 53, 1053-1061.
4. Aisen EG, Medina VH, Venturino A (2002). Cryopreservation and post- thawed fertility of ram semen frozen in different trehalose concentrations. Theriogenology, 57, 1801 - 1808.
5. Aitken RJ, Baker MA (2004). Oxidative stress and male reproductive biology. Reproduction Fertility and Development, 16, 581-588.
6. An TZ, Iwakiri M, Edashige K, Sakurai T, Kasai M (2000). Factors affecting the survival of frozen-thawed mouse spermatozoa. Cryobiology, 40, 237-249.
7. Ateşşahin A, Bucak MN, Tuncer PB, Kizil M (2008). Effects of antioxidant additives on microscopic and oxidative parameters of Angora goat semen following the freeze- thawing process. Small Ruminant Research, 77, 38-44.
8. Bailey JL, Bilodeau JF, Cormier N (2000). Semen cryopreservation in domestic animals: a damaging and capacitating phenomenon. Journal of Andrology, 21, 1-7.

9. Beconi M, Affrachino M, Schang L, Beorlegui N (1991). Influence of antioxidants on SOD activity in bovine sperm. Biochemistry International, 23, 545-553.
10. Beconi M, Francia C, Mora N, Affrachino M (1993). Effect of natural antioxidants on frozen bovine semen preservation. Theriogenology, 40, 841-851.
11. Bucak MN, Ateşşahin A, Varişli, Ö, Yüce A, Tekin, N., Akçay A (2007). The influence of trehalose, taurine, cysteamine and hyaluronan on ram semen: microscopic and oxidative stress parameters after the freeze–thawing process. Theriogenology, 67, 1060-1067.
12. Chen Y, Foote RH, Brockett CC (1993). Effect of sucrose, trehalose, hypotaurine, taurine, and blood serum on survival of frozen bull Sperm. Cryobiology, 30, 423-431.
13. Crowe LM, Mouradian R, Crowe JH, Jackson SA, Womersley C (1984). Effects of carbohydrates on membrane stability at low water activities. Biochimica et Biophysica Acta-Biomembranes, 779, 141- 150.
14. Dalimata AM, Graham JK (1997). Cryopreservation of rabbit spermatozoa using acetamide in combination with trehalose and methyl cellulose. Theriogenology, 48, 831-841.
15. Fernández-Santos MR, Martínez-Pastor F, García-Macías V, Esteso MC, Soler AJ, dePaz P, Anel L, Garde JJ (2007). Extender osmolality and sugar supplementation exert a complex effect on the cryopreservation of Iberian red deer (Cervuselaphushispanicus) epididymal spermatozoa. Theriogenology, 67, 738-753.
16. Foote RH, Chen Y, Brockett CC. Kaproth MT (1993). Fertility of bull spermatozoa frozen in whole milk extender with trehalose, taurine, or blood serum.Journal of Dairy Science 76:1908-1913.
17. Fukuhara R, Nishikawa Y (1973). Effects of pH, sperm concentration, washing and substrate concentration on respiration and motility of goat spermatozoa.Japanese Journal of Zootechnical Science, 44, 266-270.
18. Gadea J, Selles E, Marco MA, Coy P, Matas C, Romar R, Ruiz S (2004). Decrease in glutathione content in boar sperm after cryopreservation. Effect of the addition of reduced glutathione to the freezing and thawing extenders. Theriogenology, 62, 690-701.
19. Gutiérrez-Pérez O, Juárez-Mosqueda ML, Carvajal SU, Ortega MET (2009). Boar spermatozoa cryopreservation in low glycerol/ trehalose enriched freezing media improves cellular integrity. Cryobiology 2009, 58, 287-292.
20. Hammerstedt RH, Graham JK, Nolan JP (1990). Cryopreservation of mammalian sperm: what we ask them to survive. Journal of Andrology, 11, 73-88.
21. Holt WV, North RD (1994). Effects of temperature and restoration of osmotic equilibrium during thawing on the induction of plasma membrane damage in cryopreserved ram spermatozoa. Biology of Reproduction, 51, 414-424.
22. Hu JH, Li QW, Li G, Jiang ZL, Bu SH, Yang H, Wang LQ (2009).The cryoprotective effect of trehalose supplementation on boar spermatozoa quality. Animal Reproduction Science, 112:107-118.
23. Hu JH, Zan LS, Zhao XL, Li QW, Jiang ZL, Li YK, Li X (2010). Effects of trehalosesupplementation on semen quality and oxidative stress variables in frozen-thawed bovine semen. Journal of Animal Science, 88, 1657-1662.
24. Jafaroghli M, Khalili B, Farshad A, Zamiri MJ (2011). The effect of supplementation of cryopreservation diluents with sugars on the post-thawing fertility of ram semen. Small Ruminant Research, 96, 58-63.
25. Khalili B, Farshad A, Zamiri MJ, Rashidi A, Fazeli P (2009). Effects of sucrose and trehalose on the freezability of Markhoz goat spermatozoa. Asian-Australasian Journal Animal Science, 22, 1614- 1619.
26. Kozdrowski R (2009). The effect of trehalose on post-thaw viability and fertility of European brown hare (LepuseuropaeusPallas, 1778) spermatozoa. Animal Reproduction Science, 116, 326-334.
27. Leboeuf B, Restall B Salamon S (2000). Production and storage of goat semen for artificial insemination. Animal Reproduction Science, 62, 113-141.
28. Lenzi A, Gandini L, Lombardo F, Picardo M, Maresca V, Panfili E (2002). Polyunsaturated fatty acids of germ cell membranes, glutathione and glutathione-dependent enzyme-PHGPx: From basic to clinic. Contraception, 65, 301-304.
29. Liu Z, Foote RH, Brockett CC (1998). Survival of bull sperm frozen at different rates in media varying in osmolarity. Cryobiology, 37, 219- 230.
30. Matsuoka T, Imai H, Kohno H, Fukui Y (2006).Effect of bovine serum albumin in trehalose in semen diluents for improvement of frozen- thawed ram spermatozoa. Journal of Reproduction Development, 52, 675-683.
31. Medeiros CMO, Forell F, Oliveira ATD, Rodrigues JL (2002). Current status of sperm cryopreservation: why isn’t it better? Theriogenology 57, 327-344.
32. Meyers SA (2005). Spermatozoal response to osmotic stress. Animal Reproduction Science, 89, 57-74.
33. Molinia FC, Evans G, Casares PI, Maxwell WMC (1994). Effect of monosaccharides and disaccharides in Tris-based diluents on motility, acrosome integrity and fertility of pellet frozen ram spermatozoa. Animal Reproduction Science, 36, 113-22.
34. Parks JE, Graham JK (1992). Effects of cryopreservation procedures on sperm membranes. Theriogenology, 38, 209-222.
35. Patist A, Zoerb H (2005). Preservation mechanism of trehalose in food and biosystems. Colloids and Surfaces B: Biointerfaces, 40,107-113.
36. Reddy NSS, Mohanarao GJ, Atreja SK (2010). Effects of adding taurine and trehalose to a tris-based egg yolk extender on buffalo (Bubalusbubalis) sperm quality following cryopreservation. Animal Reproduction Science, 119, 183-190.
37. Salamon S, Ritar AJ (1982). Deep freezing of Angora goat semen: effects of diluent composition and method and rate of dilution on survival of spermatozoa. Australian Journal of Biological Science, 35, 295-303.
38. Schmehl MK, Vazquez IA, Graham EF (1986). The effects of nonpenetratingcryoprotectantsadded to TEST - yolk - glycerol extender on the post-thaw motility of ram spermatozoa. Cryobiology, 23, 512-517.
39. Soylu MK, Nur Z, Ustuner B, Dogan I, Sagirkaya H, Gunay U, Kemal AK (2007). Effects of various cryoprotective agents and extender osmolality on post-thawed ram semen. Bulletin of the Veterinary Institute in Pulawy, 51, 241-246.
40. Squires EL, Keith SL, Graham JK (2004). Evaluation of alternative cryoprotectants for preserving stallion spermatozoa. Theriogenology, 62, 1056-1065.
41. Storey BT, Noiles EE, Thompson KA (1998).Comparison of glycerol, other polyols, trehalose and raffinose to provide a defined cryoprotectant medium for mouse sperm cryopreservation. Cryobiology, 37, 46-58.
42. Sussman AS, Lingappa BT (1959).Role of trehalose in ascopores of NeurosporeTetrasperma. Science, 130, 1343.
43. Sztein JM, Noble K, Farley JS, Mobraaten LE (2001). Comparison of permeating and nonpermeatingcryoprotectants for mouse sperm cryopreservation.Cryobiology, 41, 28 - 39.
44. Thomas AD, Meyers SA, Ball BA (2006). Capacitation-like changes in equine spermatozoa following cryopreservation.Theriogenology, 65, 1531-1550.
45. Tonieto RA, Goularte KL, Gastal GDA, Schiavon RS, Deschamps JC, Lucia Jr T (2010). Cryoprotectant effect of trehalose and low-density lipoprotein in extenders for frozen ram semen. Small Ruminant Research, 93, 206-209.
46. Watson PF (1995). Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post- thawing function. Reproduction Fertility and Development, 7, 871- 891.
47. Watson PF (2000). The causes of reduced fertility with cryopreserved semen. Animal Reproduction Science, 60-61, 481-492.
48. Watson PF, Plummer JM, Jones PS, Bredl JC (1995). Localization of intracellular calcium during the acrosome reaction in ram spermatozoa. Molecular Reproduction and Development, 41, 513- 520.
49. Woelders H, Matthij A, Engel B (1997). Effects of trehalose and sucrose, osmolality of the freezing medium and cooling rate on viability and intactness of bull sperm after freezing and thawing. Cryobiology, 35, 93-105.
50. Yamashiro H, Narita K, Sugimura S, Han YJ, Sugawara A, Morohaku K, Nakazato F, Konno T, Yoshida M, Sato E (2007). Trehalose enhanced the freezability of Poodle dog sperm collected by an artificial vagina (AV). Animal Reproduction Science, 102, 165-171.
51. Yildiz C, Kaya A, Aksoy M, Tekeli T (2000). Influence of sugar supplementation of the extender on motility, viability and acrosomalintegrity of dog spermatozoa during freezing. Theriogenology, 54, 579-585.