The immune system organs in fishes with different feeding behavior and habitats: II. Histological studies on spleen

Year 2017, Volume: 6 Issue: 2, 519 - 525, 01.06.2017

Ülker Eren Müge Bozkurt

Abstract

Background/ Aim: The aim of this study was to investigate histological and histochemical differences of the head kidney and the splenic tissue among three fish with different feeding behavior and habitats. Material and Method: As a material, adult fifteen sea basses Dicentrarchus labrax L., 1758 and ten zanders Sander lucioperca L., 1758 , and six grass carps Ctenopharyngodon idella Val., 1844 were used. Splenic tissue samples of fishes were fixed in 10 % neutral buffered formalin NBF and Bouin’s solution. The tissue sections were stained using triple, Gordon and Sweet’s silver staining, Verhoeff’s stain, PAS reaction and methyl green pyronin staining methods. Results and Discussion: It was observed that the histological organization spleen was more organized and the white pulp areas of spleen were more pronounced in sea bass, differently from the others. The spleenic pulp structure was more confusing in zander. In the grass carp, the spelenic tissue has erytroid areas and the filled wessels with erythrocyte. Ellipsoid and periellipsoidal lymphoid tissue were also distinguished in spleen of sea bass and zander. Whereas, the splenic tissue ellipsoids were observed, but peri ellipsoid lymphoid tissues were absent in grass carp spleen. It was noted that the centers of melanomacrophages were more regular, restricted, and the content of melanin in the cells was greater in spleen of sea bass. However, the amount of melanin in melanomacrophage center was less in grass carp as compared to others. The macrophages had PAS positive reaction in three fish’s spleenic tissue, and in addition the mast cells were give PAS positive reaction in sea bass. In all three fish, pyroninophilic plasmoblasts and plasma cells were observed in spleenic tissue. Particularly, these cells were accumulated around the melanomacrophage centers and ellipsoids. Research results showed that the sea bass which carnivorous fish have more advanced defense mechanism in spleen than the others. Key words: Dicentrarchus labrax, Sander lucioperca, Ctenopharyngodon idella, head kidney, spleen, histology.

Keywords

Dicentrarchus labrax, Sander lucioperca, Ctenopharyngodon idella, head kidney, spleen, histology.

Beslenme tipi ve yaşam ortamı farklı olan balıklarda immun sistem organları: II. Dalak üzerinde histolojik çalışmalar

Abstract

Özbilgi / Amaç: Yaşam ortamı ve yeme alışkanlığı farklı olan üç balıkta, dalak dokusunun histolojik ve histokimyasal özelliklerinin karşılaştırmalı olarak incelenmesi amaçlanmıştır. Materyal ve Metot: Araştırmada materyal olarak; erişkin 15 adet deniz levreği Dicentrarchus labrax L.,1758 , 10 adet sudak Sander lucioperca L.,1758 ve 6 adet ot sazanı Ctenopharyngodon idella Val., 1844 kullanıldı. Balıklardan alınan dalak örnekleri % 10’luk tamponlu nötral formalin NBF ve Bouin tespit solusyonunda tespit edildi. Doku kesitlerine üçlü boyama yöntemi, Gordon ve Sweet’in gümüşleme metodu, Verhoeff’in elastik iplik boyama metodu, PAS reaksiyonu ve methyl green pyronin boyama yöntemleri uygulandı. Bulgular ve Sonuç: Deniz levreğinde dalağın histolojik organizasyonunun diğer balıklara göre daha düzenli olduğu, beyaz pulpa alanlarının daha belirgin olduğu görüldü. Sudak dalağında pulpanın daha karmaşık yapıda olduğu gözlendi. Ot sazanı dalağında diğerlerinden farklı olarak, eritroid alanlar tespit edildi. Damarlarda eritrosit rezervuarı dikkati çekti. Hem deniz levreği hem de sudakta elipsoid ve elipsoid çevresi lenfoid doku ayırt edildi. Ot sazanında ise dalak dokusunda elipsoidler ayırt edildi fakat elipsoid çevresi lenfoid doku gözlenmedi. Melanomakrofaj merkezlerinin levrekte daha düzenli, sınırlandırılmış şekilde olduğu ve hücrelerde melanin içeriğinin fazla olduğu dikkati çekti. Ot sazanında makrofajlardaki melanin miktarının, diğer balıklara göre az olduğu görüldü. Her üç balıkta da makrofajlar, levrekte ise ilaveten mast hücreleri de PAS pozitif reaksiyon gösterdiler. Parenşimde pirorinofilik plazmoblast ve plazma hücreleri tespit edildi. Bu hücrelerin özellikle melanomakrofaj merkezlerinin çevresinde ve elipsoidlerin etrafında yoğunlaştıkları dikkati çekti. Araştırma sonunda, karnivor bir balık olan deniz levreğinin dalağının, savunma mekanizması açısından diğer balıklara göre daha organize yapıda olduğu belirlendi.

Keywords

Dicentrarchus labrax, Sander lucioperca, Ctenopharyngodon idella, dalak, histoloji.

References

• Abbate F, Guerrera MC, Montalbano G, De Carlos F, Suárez AÁ, Ciriaco E, Germanà A. (2012). Morphology of the European sea bass (Dicentrarchus labrax) tongue. Microscopy Research and Technique, 75, 643-649.
• Barber LD, Westermann MJE (1978). Occurrence of the periodic acid- schiff positive granular leucoyte (PAS-GL) in some fishes and its significance. Journal of Fish Biology, 12(1): 35-43.
• Castro R, Tafalla C (2015). Overview of fish immunity. Mucosal Health in Aquaculture. Academic Press USA, pp. 3-54.
• Crossman G (1937). Modification of mallory’s connective tissue stain with a discussion of the principles involved. Anatomical Record, 69, 33-8.
• Cudmore B, Mandrak NE (2004). Biological synopsis of grass carp (Ctenopharyngodon idella). Canadian Manuscript Report of Fisheries and Aquatic Sciences, 2705, 44.
• Culling CFA, Alliston RT, Barr WT (1985). Cellular pathology technique. Butterworths London, UK, pp. 164-79.
• Ellis AE (2001). Innate host defense mechanisms of fish against viruses and bacteria. Developmental & Comparative Immunology, 25, 827- 839.
• Espenes A, Press CML, Dannevig BH, Landsverk T (1995a). Investigation of the structural and functional features of splenic ellipsoids in rainbow trout (Oncorhynchus mykiss). Cell and Tissue Research, 279, 469-74.
• Espenes A, Press CML, Dannevig BH, Landsverk T (1995b). Immune- complex trapping in the splenic ellipsoids of rainbow trout (Oncorhynchus mykiss). Cell and Tissue Research, 282, 41-48.
• Fournier-Betz V, Quentel C, Lamour F, LeVen A (2000). Immunocytochemical detection of Ig-positive cells in blood, lymphoid organs and the gut associated lymphoid tissue of the turbot (Scophthalmus maximus). Fish & Shellfish Immunology, 10, 187-202.
• Graf R, Schlüns J (1979). Ultrastructural and histochemical investigation of the terminal capillaries in the spleen of the carp (Cyprinus carpio L.). Cell and Tissue Research, 196, 289-306.
• Kita J, Itazawa Y (1989). Release of erythrocytes from the spleen during exercise and splenic constriction by adrenaline infusion in the rainbow trout. Japanese Journal of Ichthyology, 36, 48-52.
• Koppang EO, Fischer U, Moore L, Tranulis MA, Dijkstra JM, Köllner B, Hordvik I (2010). Salmonid T cells assemble in the thymus, spleen and in novel interbranchial lymphoid tissue. Journal of Anatomy, 217, 728-739.
• Meseguer J, Lopez-Ruiz A, Esteban MA (1994). Melano-macrophages of the seawater teleosts, sea bass (Dicentrarchus labrax) and gilthead seabream (Sparus aurata): morphology, formation and possible function. Cell and Tissue Research, 277, 1-10.
• Meseguer J, López-Ruiz A, Garcí-Ayala A (1995). Reticulo-endothelial stroma of the head-kidney from the seawater teleost gilthead seabream (Sparus aurata L.): An ultrastructural and cytochemical study. The Anatomical Record, 241, 303-9.
• Noya M, Lamas J (1996). Morphology and histochemistry of a PAS- positive granular cell in the gills of the gilthead seabream, Sparus aurata L. Journal of Anatomy, 189, 439-43.
• Ortuño J, Esteban M, Meseguer J (2002). Effects of four anaesthetics on the innate immune response of gilthead seabream (Sparus aurata L.). Fish & Shellfish Immunology, 12, 49–59.
• Özyurt CE, Mavruk S, Kiyağa VB (2012). Effects of predator size and gonad maturation on food preference and feeding intensity of Sander lucioperca (Linnaeus, 1758). Turkish Journal of Fisheries and Aquatic Sciences, 12, 315-22.
• Padrós F, Crespo S (1996). Ontogeny of the lymphoid organs in the turbot Scophthalmus maximus: a light and electron microscope study. Aquaculture,144, 1-16.
• Press CML, Dannevig BH, Landsverk T (1994). Immune and enzyme histochemical phenotypes of lymphoid and nonlymphoid cells within the spleen and head kidney of Atlantic salmon (Salmo salar L.). Fish & Shellfish Immunology, 4, 79-93.
• Quesada J, Viilena MI, Agulleiro B (1990). Structure of the spleen of the sea bass (Dicentrarchus labrax): a light and electron microscopic study. Journal of Morphology, 206, 273-281.
• Roberts RJ (1975). Melanin-containing cells of teleost fish and their relation to disease. The Pathology of Fishes, p. 399-428.
• Scapigliati G, Romano N, Buonocore F, Picchietti S, Baldassini MR, Prugnoli D, Abelli L (2002). The immune system of sea bass, Dicentrarchus labrax, reared in aquaculture. Developmental & Comparative Immunology, 26, 151-60.
• Scapigliati G, Romano N, Picchietti S, Mazzini M, Mastrolia L, Scalia D, Abelli L (1996). Monoclonal antibodies against sea bass Dicentrarchus labrax (L.) immunoglobulins: immunolocalisation of immunoglobulin-bearing cells and applicability in immunomassays. Fish & Shellfish Immunology, 6, 383-401.
• Van Muiswinkel WB, Lamers CHJ, Rombout JHWM (1991). Structural and functional aspects of the spleen in bony fish. Research in immunology, 142, 362-366.
• Vigliano FA, Bermşdez R, Quiroga MI, Nieto JM (2006). Evidence for melano-macrophage centres of teleost as evolutionary precursors of germinal centres of higher vertebrates: an immunohistochemical study. Fish & Shellfish Immunology, 21, 467-71.
• Watts M, Munday BL, Burke CM (2001). Immune responses of teleost fish. Australian Veterinary Journal, 79, 570-4.
• Zapata A, Diez B, Cejalvo T, Gutierrez-de Frias C, Cortes A (2006). Ontogeny of the immune system of fish. Fish & Shellfish Immunology, 20, 126-36.
• Zapata A, Chibà A, Varas A (1996). Cells and tissues of the immune system of fish. In: Iwama Q, Nakanishi T (eds) The fish immune system: organism, pathogen and environment. Academic Press, San Diego, Pp. 1–62.