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Tissue Microarray Technology In Pathology: Time And Cost Advantages

Year 2015, Volume: 4 Issue: 1, 364 - 367, 01.01.2015

Abstract

The tissue microarray TMA was first described by Kononen in 1998, and represents a high-throughput technology for the assessment of histology-based laboratory tests, including immunohistochemistry and fluorescent in-situ hybridization FISH . In this study, we described TMA methodology that we established in two different scientific research projects. In the first study the number of samples were 108 paraffin blocks and these blocks were designed and embedded to 10 recipient TMA blocks, in the second study; the number of samples were 700 paraffin blocks and they were designed and embedded to 30 recipient TMA blocks. Then immunohistochemical tests and analyses were performed. In the first study, 1 TUNEL staining and immunoperoxidase test IPT for 8 immunological markers, in the second study IPT for 1 immunological marker and 1 histopathological analysis were done and total time for completion the study and cost of the kits and consumables used for each section was calculated. Significantly economic gain was achieved from primary antibody and immunohistochemistry test solution, considering the number of samples and sections that used in studies. The allocated time for study is minimized by tissue microarray method. Tissue microarray method was shown as a very useful method both labor force and economical perspective in interdisciplinary studies collaborating with pathology laboratories.

References

  • Kononen J, Bubendorf L, Kallioniemi A. (1998). Tissue microarrays for high-throughput molecular profiling of tumor specimens. Nat Med;4:844–847.
  • Voduc D, Kenney C, Nielsen TO. (2008). Tissue microarrays in clinical oncology. Semin Radiat Oncol. Apr;18(2):89-97. doi: 10.1016/j.semradonc.2007.10.006.
  • Kallioniemi OP, Wagner U, Kononen J, Sauter G. (2001) Tissue microarray technology for high-throughput molecular profiling of cancer. Hum Mol Genet. Apr;10(7):657-62.
  • Brown LB, Huntsman D, (2007) Fluorescent in situ hybridization on tissue microarrays: challenges and solutions. J Mol Hist,38:151-157.
  • Singh A, Sau AK. (2010). Tissue Microarray: A powerful and rapidly evolving tool for high-throughput analysis of clinical specimens. International Journal of Case Reports and Images, 1:1-6
  • Kırıkkale Üniversitesi Bilimsel ve Teknolojik Araştırma Laboratuvarları, http://kubtal.kku.edu.tr
  • Packeisen J, Korsching E, Herbst H, Boecker W, Buerger H. (2003). Demystified...Tissue microarray technology J Clin Pathol: Mol Pathol;56:198–204
  • Shebl AM, Zalata KR, Amin MM, El-Hawary AK. (2011). An inexpensive method of small paraffin tissue microarrays using mechanical pencil tips. Diagnostic Pathology, 6:117.
  • Pires AR, Andreiuolo FD, Rabello de Souza S. (2006). TMA for all: a new method for the construction of tissue microarrays without recipient paraffin block using custom-built needles. Diagnostic Pathology,1:14
  • Ilyas M, Grabsch H, Ellis I O, Womack C, Brown R, Berney D, Fennell D, Salto-Tellez M, Jenkins M, Landberg G, Byers R, Treanor D, Harrison D, Green A R, Ball G, Hamilton P. (2013). Guidelines and considerations for conducting experiments using tissue microarrays. Histopathology 62, 827–839

Patolojide Doku Mikroarray Teknolojisi: Zaman ve Bütçe Avantajları

Year 2015, Volume: 4 Issue: 1, 364 - 367, 01.01.2015

Abstract

Doku mikroarray DMA teknolojisi ilk olarak 1998 yılında ortaya konulan ve aynı anda çok fazla sayıda doku örneğinin histolojik, immunohistokimyasal veya in situ hibridizasyon FISH testleriyle incelenmesine imkân veren yeni bir tekniktir. Sunulan bu çalışmada; yüksek örnek sayısına sahip iki ayrı bilimsel araştırma projesinde kullandığımız DMA teknolojisi tanımlanmaktadır. Birinci çalışmada 108 parafin bloktan oluşan örnek sayısı 10 DMA alıcı bloğuna, ikinci çalışmada ise 700 parafin bloktan oluşan örnek sayısı 30 DMA alıcı bloğuna yerleştirilmiş ve immunohistokimyasal test ve analizleri yapılmıştır. Birinci çalışmada; 1 TUNEL ve 8 ayrı hücre belirteci için immunoperoksidaz teknik IPT , ikinci çalışmada ise; 1 belirteç için IPT ve 1 hematoksilen eozin boyaması yapıldı. Test prosedürleri, çalışmanın tamamlanması için gereken toplam zaman ve her bir kesit için kullanılan kit ve sarf malzemelerin maliyeti hesaplandı. Özellikle çalışmalarda kullanılan örnek sayısı ve alınan kesit sayısı düşünüldüğünde, primer antikor ve immunohistokimyasal test solüsyonları açısından belirgin derecede ekonomik kazanç sağlandı. Her iki çalışma için ayrılan sürenin, doku mikroarray metodu ile belirgin derecede düştüğü ortaya çıktı. Sonuç olarak, doku mikroarray yönteminin yüksek örnek ve test sayısına sahip çalışmalarda, hem işgücü ve hem de ekonomik perspektif açısından çok kullanışlı bir yöntem olduğu gösterildi.

References

  • Kononen J, Bubendorf L, Kallioniemi A. (1998). Tissue microarrays for high-throughput molecular profiling of tumor specimens. Nat Med;4:844–847.
  • Voduc D, Kenney C, Nielsen TO. (2008). Tissue microarrays in clinical oncology. Semin Radiat Oncol. Apr;18(2):89-97. doi: 10.1016/j.semradonc.2007.10.006.
  • Kallioniemi OP, Wagner U, Kononen J, Sauter G. (2001) Tissue microarray technology for high-throughput molecular profiling of cancer. Hum Mol Genet. Apr;10(7):657-62.
  • Brown LB, Huntsman D, (2007) Fluorescent in situ hybridization on tissue microarrays: challenges and solutions. J Mol Hist,38:151-157.
  • Singh A, Sau AK. (2010). Tissue Microarray: A powerful and rapidly evolving tool for high-throughput analysis of clinical specimens. International Journal of Case Reports and Images, 1:1-6
  • Kırıkkale Üniversitesi Bilimsel ve Teknolojik Araştırma Laboratuvarları, http://kubtal.kku.edu.tr
  • Packeisen J, Korsching E, Herbst H, Boecker W, Buerger H. (2003). Demystified...Tissue microarray technology J Clin Pathol: Mol Pathol;56:198–204
  • Shebl AM, Zalata KR, Amin MM, El-Hawary AK. (2011). An inexpensive method of small paraffin tissue microarrays using mechanical pencil tips. Diagnostic Pathology, 6:117.
  • Pires AR, Andreiuolo FD, Rabello de Souza S. (2006). TMA for all: a new method for the construction of tissue microarrays without recipient paraffin block using custom-built needles. Diagnostic Pathology,1:14
  • Ilyas M, Grabsch H, Ellis I O, Womack C, Brown R, Berney D, Fennell D, Salto-Tellez M, Jenkins M, Landberg G, Byers R, Treanor D, Harrison D, Green A R, Ball G, Hamilton P. (2013). Guidelines and considerations for conducting experiments using tissue microarrays. Histopathology 62, 827–839
There are 10 citations in total.

Details

Primary Language Turkish
Journal Section Research Article
Authors

Hasan Tarık Atmaca This is me

Oğuz Kul This is me

Sıla Canpolat This is me

Tuğçe Anteplioğlu This is me

Publication Date January 1, 2015
Published in Issue Year 2015 Volume: 4 Issue: 1

Cite

APA Atmaca, H. T., Kul, O., Canpolat, S., Anteplioğlu, T. (2015). Patolojide Doku Mikroarray Teknolojisi: Zaman ve Bütçe Avantajları. Animal Health Production and Hygiene, 4(1), 364-367. https://doi.org/10.1016/j.semradonc.2007.10.006.