High Trehalose Concentration in Tris-Based Egg Yolk Extender has Detrimental Effect on Post-Thaw Semen Quality in Nili-Ravi Buffalo Bull

Year 2013, Volume: 2 Issue: 2, 213 - 215, 01.06.2013

Ejaz Ahmad Zahid Naseer Nasim Ahmad

Abstract

Background/Aim: The use of artificial insemination with cryopreserved semen is potential tool for breed improvement in buffalo production. However, the fertility with the use of cryopreserved semen is approximately 40% in buffaloes. It is the general opinion that a substantial number 50% of sperm damaged or dead during freezing and thawing process, is the potential cause of low fertility. The aim of the present study was to determine the effect of high trehalose concentration in Tris-based egg yolk extender on post-thawed semen quality in buffalo bull. Material and Method: Semen ejaculates n=04 collected from two mature buffalo bulls were extended with E1 Tris-citric acid-fructose TCF with 7% glycerol , E2 TCF plus 236 mM trehalose or E3 E2 plus 7% glycerol . All the extenders contained egg yolk 20%, v/v . After dilution, samples were cooled to 4°C and equilibrated for 2 hrs at 4°C. Samples were packed in 0.5 ml straws, frozen and stored in liquid nitrogen. Frozen straws were then thawed at 37°C for 20 sec. Motility was observed after dilution, equilibration and post-thawing. Plasma membrane integrity and percent live sperm were determined after thawing by using hypo-osmotic swelling assay and eosin-nigrosin staining, respectively. Results and Conclusion: The results showed that the motility was decreased p

Keywords

Buffalo semen, Trehalose, Glycerol, Post-thaw semen quality.

Tris-Yumurta Sarısı Sulandırıcısına Yüksek Düzeyde Trehaloz İlavesi Nili-Ravi Manda Spermalarında Çözüm Sonu Parametreleri Olumsuz Etkiler

Abstract

Özbilgi/Amaç: Dondurulmuş sperma ile suni tohumlamanın manda yetiştiriciliği ve üretiminde kullanımı önemli bir yöntemdir. Buna karşın, mandalarda dondurulmuş sperma ile fertilite oranı yaklaşık %40 dolayındadır. Fertilitedeki düşüş genel olarak dondurma ve çözdürme sırasında spermanın %50’sinin hasar görmesine yorumlanmıştır. Bu çalışmada yüksek trehaloz yoğunluklarının mandalarda çözüm sonu sperma kalitesi üzerine etkisinin belirlenmesi amaçlanmıştır. Materyal ve Metot: Toplam iki baş erişkin manda boğasından alınan sperma n=04 örnekleri E1 Tris-sitrik asit-fruktoz+%7 gliserol TCF , E2 TCF+236mM trehaloz veya E3 E2+%7 glycerol sulandırıcılarıyla sulandırıldı. Bu üç sulandırıcı da %20 v/v oranında yumurta sarısı içermekteydi. Daha sonra örnekler 4C°’ye kadar soğutuldu ve bu sıcaklıkta iki saat süre ile ekilibre edildi. Sperma örnekleri 0,5 ml lik payetlere çekildi ve sıvı azot içerisinde dondurularak saklandı. Payetler 37C°’de 20 saniyede çözdürüldü. Motilite değerleri sulandırma, ekilibrasyon ve çözüm sonrasında belirlendi. Plazma membran bütünlüğü ve canlı spermatozoon oranları, sırasıyla, hipoozmotik şişme testi ve eosin-nigrosin boyama yöntemiyle belirlendi. Bulgular ve Sonuç: Sulandırma, ekilibrasyon ve çözüm sonu motilite değerleri E2 ve E3 sulandırıcılarında E1’e göre daha düşük olarak belirlendi P

Keywords

Manda sperması, Trehaloz, Gliserol, Çözüm sonu sperma kalitesi.

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