Determination of Levels of Some Acute Phase Proteins, Tumor Necrosis Factor-α, Interleukin-1 and Interleukin-6 in Cattle with Trichophythosis

Year 2023, Volume: 12 Issue: 1, 15 - 19, 23.06.2023

Cansu Çakır Zoroğlan , Oğuz Merhan

https://doi.org/10.53913/aduveterinary.1219029

Abstract

Our aim in the study was to determine the levels of some acute phase proteins (APP) and proinflammatory cytokines in cattle with trichophytosis. The animal material of the study consisted of total 30 cattle, 15 with trichophytosis and 15 healthy. The blood samples taken from the Vena jugularis of the cattle in the study into tubes without anticoagulant were centrifuged at 3000 rpm for 15 minutes and their serum samples were obtained. Serum samples were stored at -20 °C until analysis. In the obtained serum samples, haptoglobin, serum amyloid A (SAA), ceruloplasmin, albumin, total protein, tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1) and interleukin-6 (IL-6) analyzes were performed. It was determined that haptoglobin, SAA, TNF-α, IL-1, IL-6 and ceruloplasmin levels were statistically increased in cattle with trichophytosis compared to the control group. In addition, albumin, total protein and globulin values were lower than the control group, but the difference between the groups was statistically insignificant. As a result, it is thought that trichophytosis causes changes in biochemical parameters in cattle, the use of biochemical parameters, especially APPs, will contribute to the diagnosis of trichophytosis and more detailed studies should be done on this subject.

Keywords

Acute phase proteins, cattle, proinflammatory cytokine, trichophytosis.

References

• Apaydin Yildirim, B. (2020). Evaluation of biochemical parameters and oxidative stress in native and crossbred cattle naturally infected with Dermatophytosis. GSC Biological and Pharmaceutical Sciences, 13(02), 099-104. https://doi.org/10.30574/gscbps.2020.13.2.0357
• Arslan, H.H., Yarım, G.F., Yavuz, O., & Bas, B. (2007). Positive effects of attenuated Trichophyton verrucosum strain administration in treatment of the bovine trichophytosis. Revue de Medecine Veterinaire, 158(10): 509-513.
• Atakisi, E., Karapehlivan, M., Atakisi, O., Kontas, T., & Marasli, Ş. (2006). Adenosine deaminase and biochemical liver function tests in the dermatophytic cattle. Bulletin of the Veterinary Institute in Puławy, 50(4), 481-483.
• Balıkçı, E., & Gazioğlu, A. (2017). Trikofitozisli sığırlarda haptoglobin ve serum amyloid A düzeyleri ve Nigella sativa’nın antiinflamatuar etkisi. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, 31(2), 93-96.
• Bozukluhan, K. (2014). Deri hastalıkları. In A. Kırbaş, A.D. Ömür, E. Doğan (Eds.), Sığır Hastalıkları (pp. 207-216). Medipres, Malatya.
• Bozukluhan, K., & Gökçe, H.İ. (2007). Retikuloperitonitis travmatika ve perikarditis travmatika’lı sığırlarda bazı akut faz proteinlerin araştırılması. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 4(2), 107-113.
• Bozukluhan, K., & Merhan, O. (2022). Clinical significance of some acute phase proteins in cattle. In A. Kükürt, & V. Gelen (Eds.), Cattle Diseases - Molecular and Biochemical Approach (pp.1-13). Intech Open. http://dx.doi.org/10.5772/intechopen.108152
• Bozukluhan, K., Merhan, O., Kiziltepe, S., Ergin Egritag, H., Akyuz, E., & Gokce, H.I. (2021). Determination of haptoglobin, some biochemical and oxidative stress parameters in calves with pneumonia. Fresenius Environmental Bulletin, 30, 9485-9489.
• Bozukluhan, K., Merhan, O., Özcan, A., Gökçe, H.İ., & Gökce, G. (2017). Toxocara vitulorum ile doğal enfekte buzağılarda serum haptoglobin düzeyi, oksidatif belirteçler ve bazı biyokimyasal parametrelerin düzeyinin belirlenmesi. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 64: 75-79. https://doi.org/10.1501/Vetfak_0000002778
• Brodzki, P., Marczuk, J., Lisiecka, U., Szczubial, M., Brodzki, A., Gorzkos, H., & Kulpa, K. (2021). Comparative evaluation of cytokine and acute-phase protein concentrations in sera of dairy cows with subclinical and clinical ketosis as a different view of the causes of the disease. Veterinary World, 14(6), 1572-1578. https://doi.org/10.14202/vetworld.2021.1572-1578
• Ceciliani, F., Giordano, A., & Spagnolo, V. (2002). The systemic reaction during inflammation: the acute-phase proteins. Protein and Peptide Letters, 9(3), 211-223. https://doi.org/10.2174/0929866023408779
• Colombo, J.P., & Richterich, R. (1964). Zur bestimmung des caeruloplasmin im plasma (on the determination of ceruloplasmin in plasma). Schweizerische Medizinische Wochenschrift, 94, 715-720.
• Constable, P.D., Hinchcliff, K.W., Done, S.H., & Grünberg, W. (Eds.). (2017). Veterinary medicine: a textbook of the diseases of cattle, horses, sheep, pigs, and goats. Elsevier, China.
• Gökçe, G., Şahin, M., Irmak, K., Otlu, S., Aydın, F., & Genç, O. (1999). Sığır trichophytosis’inde profilaktik ve terapötik amaçla aşı kullanımı. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 5(1), 81-86.
• Gökçe, H.İ., & Bozukluhan, K. (2009). Çiftlik hayvanlarında önemli akut faz proteinleri ve bunların veteriner hekimlik alanındaki kullanımı. Dicle Üniversitesi Veteriner Fakültesi Dergisi, 1(1), 1-14.
• Hellman, N.E., & Gitlin, J.D. (2002). Ceruloplasmin metabolism and function. Annual Review of Nutrition, 22, 439-458. https://doi.org/10.1146/annurev.nutr.22.012502.114457
• Kabu, M., & Koca, H.B. (2018). The levels of some biochemical parameters in anatolian buffaloes with trichophytosis. Kocatepe Veterinary Journal, 11(2), 156-159. https://doi.org/10.30607/kvj.402897
• Kabu, M., & Sayın, Z. (2016). Concentrations of serum amyloid A, haptoglobin, tumour necrosis factor and interleukin-1 and -6 in Anatolian buffaloes naturally infected with dermatophytosis. Veterinarni Medicina, 61(3), 133-135. https://doi.org/10.17221/8770-VETMED
• Karapehlivan, M., Uzlu, E., Kaya, N., Kankavi, O., Ural, K., & Çitil, M. (2007). Investigation of some biochemical parameters and the antioxidant system in calves with dermatophytosis. The Turkish Journal of Veterinary and Animal Sciences, 31(2), 85-89.
• Katoh, N., Oikawa, S., Oohashi, T., Takahashi, Y., Itoh, F. (2002). Decreases of apolipoprotein B-100 and A-I concentrations and induction of haptoglobin and serum amyloid A in nonfed calves. The Journal of Veterinary Medical Science, 64(1), 51-55.
• Kumar, A., & Khurana, R. (2002). Effect of dermatophytosis on haematobiochemical parameters in cattle. The Haryana Veterinarian, 41, 44-47.
• Merhan, O., & Bozukluhan, K. (2022). Acute phase response and some acute phase proteins in animals. In G. Yıldız, M.S. Baran, O. Kaplan, & Ö. Durna Aydın (Eds.), Current Multidisciplinary Studies in Veterinary Medicine I (pp. 3-33). Iksad Publishing House, Ankara.
• Merhan, O., Bozukluhan, K., & Gokce, H.I. (2017a). Acute phase proteins and biochemical and oxidative stress parameters in Hypoderma spp. infested cattle. Journal of the Hellenic Veterinary Medical Society, 68(4), 535-540. http://dx.doi.org/10.12681/jhvms.16049
• Merhan, O., Bozukluhan, K., Çelebi, Ö., Öğün, M., Atakişi, E., & Büyük, F. (2017b). Levels of acute phase protein and some biochemical parameter in cattle infected with Mycobacterium bovis. Journal of The Faculty of Veterinary Medicine Erciyes University, 14(2), 101-105.
• Merhan, O., Bozukluhan, K., Gökçe, G., & Yılmaz, O. (2016). İshalli buzağılarda haptoglobin, seruloplazmin ve bazı biyokimyasal parametre düzeylerinin araștırılması. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, 30(3), 195-198.
• Merhan, O., Bozukluhan, K., Kiziltepe, S., & Gokce, H.I. (2017c). Investigation of levels of haptoglobin, serum amyloid A, ceruloplasmin and albumin in cattle with foot-and-mouth disease. Israel Journal of Veterinary Medicine, 72(4), 14-17.
• Molina, E.C. (2005). Serum interferon-gamma and interleukins-6 and -8 during infection with Fasciola gigantica in cattle and buffaloes. Journal of Veterinary Science, 6(2), 135-139.
• Muhsin, T.M., Aubaid, A.H., & al-Duboon, A.H. (1997). Extracellular enzyme activities of dermatophytes and yeast isolates on solid media. Mycoses, 40(11-12), 465-469. https://doi.org/10.1111/j.1439-0507.1997.tb00186.x
• Murata, H., Shimada, N., & Yoshioka, M. (2004). Current research on acute phase proteins in veterinary diagnosis: an overview. The Veterinary Journal, 168(1), 28-40. https://doi.org/10.1016/S1090-0233(03)00119-9
• Özkanlar, Y.E., Aktas, M.S., & Kirecci, E. (2009). Mycozoonozis associated with ringworm of calves in Erzurum province Turkey. Kafkas Universitesi Veteriner Fakültesi Dergisi, 15(1), 141-144.
• Papini, R., Nardoni, S., Fanelli, A., & Mancianti, F. (2009). High infection rate of Trichophyton verrucosum in calves from Central Italy. Zoonoses and Public Health, 56(2), 59-64. https://doi.org/10.1111/j.1863-2378.2008.01157.x
• Petersen, H.H., Nielsen, J.P., & Heegaard, P.M.H. (2004). Application of acute phase protein measurements in veterinary clinical chemistry. Veterinary Research, 35(2), 163-187. https://doi.org/10.1051/vetres:2004002
• Schaufuss, P., & Steller, U. (2003). Haemolytic activities of Trichophyton species. Medical Mycology, 41(6), 511-516. https://doi.org/10.1080/1369378032000141462.
• Şeliman, N. (2018). Dermatofitozisli sığırlarda tedavi öncesi ve sonrası serum amyloid A, haptoglobin ve seruloplazmin düzeylerinin ölçülmesi. [Yüksek Lisans Tezi, Kafkas Üniversitesi Sağlık Bilimleri Enstitüsü]. Kars.
• Tothova, C., Nagy, O., & Kovac, G. (2014). Acute phase proteins and their use in the diagnosis of diseases in ruminants: a review. Veterinarni Medicina, 59, 163-180. https://doi.org/10.17221/7478-VETMED
• Ulutaş, P.A., Ulutaş, B., Sarıerler, M., & Bayramlı, G. (2007). Serum haptoglobin and ceruloplasmin concentrations in dogs with various diseases. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, 33(2), 35-42.
• Witkowska-Pilaszewicz, O.D., Zmigrodzka, M., Winnicka, A., Miskiewicz, A., Strzelec, K., & Cywinska, A. (2019). Serum amyloid A in equine health and disease. Equine Veterinary Journal, 51(3), 293-298. https://doi.org/10.1111/evj.13062
• Yıldırım, M., Çınar, M., Ocal, N., Yağcı, B.B., & Askar, S. (2010). Prevalence of clinical dermatophytosis and oxidative stress in cattle. Journal of Animal and Veterinary Advances, 9(14), 1978-1982. https://doi.org/10.3923/javaa.2010.1978.1982
• Yılmazer, R.E., & Aslan, Ö. (2010). Sığırlarda mantar hastalığının sağaltımında Neguvon ve whitfield’s merhemin birlikte kullanımının etkinliğinin araştırılması. Sağlık Bilimleri Dergisi, 19(3), 175-183.