LCMSMS Analysis of 8-OHdG and Measuring Metallothionein Level for Evaluation of Protective Role of Geraniol in Lead Acetate Administered Rats

Year 2020, , 100 - 111, 25.06.2020

Aysel Alkan Uçkun , Ertan Yoloğlu , Miraç Uçkun , Ahmet Özkaya

https://doi.org/10.37094/adyujsci.697784

Abstract

    In this study, DNA damage and metallothionein levels were used as biomarkers to evaluate the protective potential of geraniol, a monoterpen against lead stress, in rats. Hepatic 8-hydroxy-2-deoxyguanosine (8-OHdG) level used as a marker of oxidative DNA damage was measured by LCMSMS. Experimental groups were formed in four ways: control, lead acetate, geraniol and lead acetate + geraniol. Seven animals were used in each group. Geraniol and lead acetate were administered to rats for 30 days. In geraniol administered rats, 8-OHdG and metallothionein levels decreased significantly compared to lead acetate administered rats. The highest DNA damage and metallothionein levels were observed in lead acetate administered rats. According to the results of this study, it can be suggested that geraniol protects cells against lead-caused damage by reducing ROS production. In addition, studies on the measurement of 8-OHdG by LCMSMS in the literature are limited. Therefore, it is thought that the presented study will contribute to the evaluation of the applicability of this method in the literature.

Keywords

8-hydroxy-2-deoxyguanosine, Metallothionein, Geraniol, Lead, LCMSMS

Project Number

FEFYL/2015–0005

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