Öjenolün Alkol ile Muamele Edilen Sıçan Karaciğeri Üzerindeki Histopatolojik ve Biyokimyasal Etkileri

Year 2020, , 83 - 99, 25.06.2020

Hasan Yıldız , Eaylettin Öztürk

https://doi.org/10.37094/adyujsci.729426

Cited By: 1

Abstract

Yüksek antioksidan kapasiteye sahip olan Öjenol maddesinin tek başına ve bir oksidatif stres etkeni olan etil alkol ile beraber tüketilmesi sonucu karaciğer hasarlanması ve oksidatif stres düzeyindeki etkileri histopatolojik ve biyokimyasal olarak değerlendirildi. Bu çalışmada, 300-390 g ağırlığında 40 adet Wistar albino dişi sıçan kullanıldı. Sıçanlar rastgele 10’arlı 4 gruba ayrıldı ve standart pellet tipi yem ile ad libitum olarak beslendi. 1. Grup (Kontrol grubu), diğer gruplar ile aynı stresi oluşturmak amacı ile 3 ml Serum fizyolojik (Sf)/gün gavaj ile 2. Grup (Etil alkol grubu), %40’lık Etil alkol 3 ml/gün gavaj ile 3. Grup (Öjenol Grubu), 50 mg/kg/gün gavaj ile, 4. Grup (Etik alkol + Öjenol grubu), %40’lık Etil alkol 3 ml/gün gavaj + 50 mg/kg/gün gavaj ile öjenol 3 ml/gün gavaj yoluyla olacak şekilde 30 gün boyunca verildi. Çalışma sonunda sıçanlardan alınan kan ve karaciğer dokusunda biyokimyasal analizler ve histolojik preparatlar hazırlandı. Öjenol, etil alkol ile beraber tüketildiğinde karaciğer üzerinde etanolün oluşturduğu hepatotoksisiteyi azalttığı tespit edildi. Öjenol grubu sıçanlarda alkol grubu sıçanlara kıyasla AST, ALT, ALP, LDH ve TRİG seviyelerinde istatistiksel olarak anlamlı bir fark bulundu (p<0.05). Ancak BİLD, BİLT, CHOL, AFP ve CEA125 seviyelerinde gruplar arasında anlamlı bir fark bulunamadı (p<0.05). Tek başına Öjenol kullanımı TAS değerini arttırırken TOS değerlerini azalttığı belirlendi. Elde ettiğimiz biyokimyasal verilere göre, öjenolün, oksidan-antioksidan dengesini oksidanlar lehine değiştirerek etanol uygulaması sonucunda oluşan hepatotoksisiteyi azalttığını söylemek mümkündür. Karaciğerin histopatolojik incelemesinden elde edilen sonuçlar, biyokimyasal verileri destekler nitelikte olup Öjenolün, etanol ile beraber tüketildiğinde karaciğer üzerinde hepatotoksik etkileri azalttığı görüldü

Keywords

Karaciğer, Öjenol, Etanol, Hepatotoksisite, Antioksidan, Oksidatif stres

Supporting Institution

Hatay Mustafa Kemal Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü (HMKÜ-BAP)

Project Number

18.D.008

Thanks

Hatay Mustafa Kemal Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü (HMKÜ-BAP)

Histopathological and Biochemical Effects of Eugenol on Alcohol-Treated Rat Liver

Abstract

    The effects of eugenol, which has a high antioxidant capacity, alone and together with ethyl alcohol, an oxidative stress factor, were evaluated histopathologically and biochemically. In this study, 40 Wistar albino female rats weighing 300-390 g were used. Rats were randomly divided into 4 groups of 10 and fed with standard pellet type feed as ad libitum. Group 1 (Control group) with 3 ml Serum physiological (Sf)/day via gavage to create the same stress as other groups, Group 2 (Ethyl alcohol group), 40% Ethyl alcohol with 3 ml/day via gavage, Group 3 (Eugenol Group) with 50 mg/kg/day via gavage, Group 4 (Ethical alcohol + Eugenol group) was given for 30 days as 40% Ethyl alcohol 3 ml/day via gavage + 50 mg/kg/day via gavage eugenol 3 ml/day via gavage. At the end of the study, biochemical analyzes and histological preparations were made in blood and liver tissue from rats. When eugenol is consumed together with ethyl alcohol, it was found that ethanol reduces hepatotoxicity on the liver. A statistically significant difference was found in AST, ALT, ALP, LDH and TRIG levels in the eugenol rats compared to the alcohol group rats (p < 0.05). However, no significant difference was found between the groups at BILD, BILT, CHOL, AFP and CEA125 levels (p < 0.05). The use of eugenol alone increased the value of TAS. It was determined that the use of eugenol alone decreased the TOS values while increasing the TAS value. According to the biochemical data we obtained, it is possible to say that eugenol reduces the hepatotoxicity formed as a result of ethanol application by changing the oxidant-antioxidant balance in favor of oxidants. The results obtained from histopathological examination of the liver support biochemical data and eugenol have been shown to reduce hepatotoxic effects on the liver when consumed with ethanol.

Keywords

Liver, Eugenol, Ethanol, Hepatotoxicity, Antioxidant, Oxidative stress

Project Number

18.D.008

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