Comparatively Investigation of Textile Dye Decolorization by a White Rot Fungus and Various Bacterial Strains

Year 2021, Volume: 11 Issue: 2, 350 - 361, 31.12.2021

Emre Birhanlı , Özfer Yeşilada , Doç. Dr. Ahmet Çabuk , Filiz Boran , Eray Tatlıcı

https://doi.org/10.37094/adyujsci.973203

Abstract

The aim of this study is to comparatively investigate the decolorization of Reactive Blue 171 (RB 171) by using three different bacterial strains as Bacillus megaterium A1 (A1), Gordonia sp. MC-D1 (D1) and Bacillus pumilus D3 (D3) and also a white rot fungus (Pleurotus ostreatus). All tested bacteria were incubated with RB 171 dye at 150 mg/L concentration for 24-72 h under static or agitated (150 rpm) conditions while the fungus was incubated with 150 mg/L of the dye under the same conditions for 3-24 h. The highest bacterial decolorization values were obtained after 72 h of incubation under static conditions and the maximum decolorization rates were detected as 84, 83 and 75% for A1, D1 and D3, respectively. However, the color of RB 171 dye was removed at 93% rate by Pleurotus ostreatus under static conditions after 24 h of incubation. Similar results were also obtained from the agitated studies of the tested fungal and bacterial strains except A1. The maximum decolorization values obtained with A1, D1 and D3 at 150 rpm, 72 h were 30, 88 and 89%, respectively. The highest decolorization activities of Pleurotus ostreatus were 93% for both static and agitated (150 rpm) conditions. In addition, zymogram analyzes of the fungal culture fluids obtained from SBM and SBM containing 150 mg/L RB 171 were also performed to detect the presence of laccase.

Keywords

Bacterium, Decolorization, Textile dye, White rot fungus, Zymogram

Supporting Institution

Inonu University Research Fund Unit

Project Number

2013/66

Thanks

We would like to thank the Inonu University Research Fund Unit for their financial support (2013/66) throughout this study.

Bir Beyaz Çürükçül Fungus ve Çeşitli Bakteriyel Suşlar ile Tekstil Boyasının Renginin Gideriminin Karşılaştırmalı Olarak Araştırılması

Abstract

Bu çalışmanın amacı, üç farklı bakteriyel suş Bacillus megaterium A1 (A1), Gordonia sp. MC-D1 (D1) and Bacillus pumilus D3 (D3) ve ayrıca bir beyaz çürükçül fungusun (Pleurotus ostreatus) kullanımı ile Reaktif Mavi 171’ in (RM 171) renginin gideriminin karşılaştırmalı olarak araştırılmasıdır. Test edilen tüm bakteriler 150 mg/L konsantrasyonda RM 171 boyası ile 24-72 saat boyunca statik ve çalkalamalı (150 rpm) koşullarda inkübe edilirken fungus 150 mg/L boya ile aynı koşullarda 3-24 saat boyunca inkübe edildi. En yüksek bakteriyel renk giderimleri statik koşullarda 72 saat inkübasyon sonrasında elde edildi ve maksimum renk giderim oranları A1, D1 ve D3 için sırasıyla 84, 83 ve %75 olarak saptandı. Bununla birlikte, Pleurotus ostreatus ile statik koşullarda, 24 saat inkübasyon sonrası RM 171 boyasının rengi %93 oranında giderildi. Benzer sonuçlar A1 hariç test edilen fungal ve bakteriyel suşların çalkalamalı çalışmalarından da elde edildi. A1, D1 ve D3 ile 150 rpm ve 72 saatte elde edilen maksimum renk giderim değerleri sırasıyla 30, 88 ve %89’ dur. Pleurotus ostreatus’ un en yüksek renk giderim aktiviteleri hem statik hem de çalkalamalı (150 rpm) koşullarda %93 idi. Ayrıca lakkaz varlığının tespiti amacıyla SBM ve 150 mg/L RB 171 içeren SBM’den elde edilen fungal kültür sıvılarının zimogram analizleri de yapıldı.

Keywords

Bakteri, Renk giderimi, Tekstil boyası, Beyaz çürükçül fungus, Zimogram

Project Number

2013/66

References

• [1] Karim, M.E., Dhar, K., Hossain, M.T., Decolorization of textile reactive dyes by bacterial monoculture and consortium screened from textile dyeing effluent, Journal of Genetic Engineering and Biotechnology, 16 (2), 375-380, 2018.
• [2] Robinson, T., Chandran, B., Nigam, P., Removal of dyes from an artificial textile dye effluent by two agricultural waste residues, corncob and barley husk, Environment International, 28 (1-2), 29-33, 2002.
• [3] Elshafei, A.M., Elsayed, M.A., Hassan, M.M., Haroun, B.M., Othman, A.M., Farrag, A.A., Biodecolorization of six synthetic dyes by Pleurotus ostreatus ARC280 laccase in presence and absence of hydroxybenzotriazole (HBT), Annual Research and Review in Biology, 15, 1-16, 2017.
• [4] Hu, T.L., Wu, S.C., Assessment of the effect of azo dye RP2B on the growth of a nitrogen fixing cyanobacterium-Anabaena sp., Bioresource Technology, 77 (1), 93-95, 2001.
• [5] Dogan, E.E., Yesilada, E., Ozata, L., Yologlu, S., Genotoxicity testing of four textile dyes in two crosses of Drosophila using wing somatic mutation and recombination test, Drug and Chemical Toxicology, 28 (3), 289-301, 2005.
• [6] Saratale, R.G., Saratale, G.D., Chang, J.S., Govindwar, S.P., Bacterial decolorization and degradation of azo dyes: A review, Journal of the Taiwan Institute of Chemical Engineers, 42 (1), 138-157, 2011.
• [7] Banat, I.M., Nigam, P., Singh, D., Marchant, R., Microbial decolorization of textile-dye-containing effluents: A review, Bioresource Technology, 58 (3), 217-227, 1996.
• [8] Yesilada, O., Birhanli, E., Ozmen, N., Ercan, S., Highly stable laccase from repeated-batch culture of Funalia trogii ATCC 200800, Applied Biochemistry and Microbiology, 50 (1), 55-61, 2014.
• [9] Meerbergen, K., Willems, K.A., Dewil, R., Impe, J.V., Appels, L., Lievens, B., Isolation and screening of bacterial ısolates from wastewater treatment plants to decolorize azo dyes, Journal of Bioscience and Bioengineering, 125 (4), 448-456, 2018.
• [10] Singh, R.P., Singh, P.K., Singh, R.L., Bacterial decolorization of textile azo dye acid orange by Staphylococcus hominis RMLRT03, Toxicology International, 21 (2), 160-166, 2014. [11] Parmar, N., Shukla, S.R., Microbial decolorization of reactive dye solutions, Clean-Soil Air Water, 43 (10), 1426-1432, 2015.
• [12] Yeşilada, Ö., Birhanlı, E., Ercan, S., Özmen, N., Reactive dye decolorization activity of crude laccase enzyme from repeated-batch culture of Funalia trogii. Turkish Journal of Biology, 38 (1), 103-110, 2014.
• [13] Boran, F., Birhanlı, E., Yeşilada, Ö., Özbey, E., Comparison of indigo carmine decolorization by Pseudomonas aeruginosa and crude laccase enzyme from Funalia trogii, Turkish Journal of Biology, 43 (1), 37-46, 2019.
• [14] Akpinar, M., Urek, R.O., Induction of fungal laccase production under solid state bioprocessing of new agroindustrial waste and its application on dye decolorization, 3 Biotech, 7 (2), 1-10, 2017.
• [15] Jasińska, A., Góralczyk-Bińkowska, A., Soboń, A., Długoński J., Lignocellulose resources for the Myrothecium roridum laccase production and their integrated application for dyes removal. International Journal of Environmental Science and Technology, 16, 4811-4822, 2019.
• [16] Liu, J., Sun, S., Han, Y., Meng, J., Chen, Y., Yu, H., Zhang, X., Ma, F., Lignin waste as co-substrate on decolorization of azo dyes by Ganoderma lucidum, Journal of the Taiwan Institute of Chemical Engineers, 122, 85-92, 2021.
• [17] Kahraman, S., Kuru, F., Dogan, D., Yesilada, O., Removal of indigo carmine from an aqueous solution by fungus Pleurotus ostreatus, Archives of Environmental Protection, 38 (3), 51-57, 2012.
• [18] Yesilada, O., Yildirim, S.C., Birhanli, E., Apohan. E., Asma, D., Kuru, F., The evaluation of pre-grown mycelial pellets in decolorization of textile dyes during repeated batch process, World Journal of Microbiology and Biotechnology, 26 (1), 33-39, 2010.
• [19] Ulu, A., Birhanli, E., Boran, F., Köytepe, S., Yesilada, O., Ateş, B., Laccase-conjugated thiolated chitosan-Fe3O4 hybrid composite for biocatalytic degradation of organic dyes, International Journal of Biological Macromolecules, 150, 871-884, 2020.
• [20] Tripathi, A., Srivastava, S.K., Ecofriendly treatment of azo dyes: biodecolorization using bacterial strains, International Journal of Bioscience, Biochemistry and Bioinformatics, 1(1), 37-40, 2011.
• [21] Sneha, U., Poornima, R., Sridhar, S., Decolorization of synthetic textile dyes using Pseudomonas putida, Journal of Chemical and Pharmaceutical Research, 5(5), 219-225, 2013.
• [22] Singh, R.P., Singh, P.K., Singh, R.L., Bacterial decolorization of textile azo dye acid orange by Staphylococcus hominis RMLRT03, Toxicology International, 21(2), 160-166, 2014.
• [23] Li, G., Peng, L., Ding, Z., Liu, Y, Gu, Z., Zhang, L., Shi, G., Decolorization and biodegradation of triphenylmethane dyes by a novel Rhodococcus qingshengii JB301 isolated from sawdust, Annals of Microbiology, 64, 1575-1586, 2014.
• [24] Ganapathy, B., Chanderan, I., Ponnaiah, P., Decolorizing palm oil mill effluent (pome) using plant polysaccharide degrading microorganisms isolated from soil, Polish Journal of Environmental Studies, 27 (2), 629-636, 2018.
• [25] Barathi, S., Aruljothi, K.N., Karthik, C., Padikasan, I.A., Optimization for enhanced ecofriendly decolorization and detoxification of reactive blue 160 textile dye by Bacillus subtilis, Biotechnology Reports 28, e00522, 1-7, 2020.
• [26] Chen, K.C., Wu, J.Y, Liou, D.J., Hwang, S.C.J., Decolorization of the textile dyes by newly isolated bacterial strains, Journal of Biotechnology, 101 (1), 57-68, 2003.
• [27] Gangavarapu, V.L., Ravuri, J.M., Decolourization of reactive violet 13 and reactive blue 171 by Pseudomonas stutzeri RJVL 1514 isolated from dye contaminated soil, International Journal of Microbiology Research, 8 (5), 754-758, 2016.
• [28] Gangavarapu, V.L., Ravuri, J.M., Decolourisation of reactive violet 13 and reactive blue 171 by Bacillus cereus RJVL 2514 isolated from dye contaminated soils, International Journal of Environmental Sciences, 7 (1), 30-39, 2016.
• [29] Vantamuri, A.B., Kaliwal, B.B., Decolourization and biodegradation of navy blue her (reactive blue 171) dye from Marasmius sp. BBKAV79, 3 Biotech, 7 (1), 1-7, 2017.
• [30] Saratale, R.G., Saratale, G.D., Chang, J.S., Govindwar, S.P., Decolorization and biodegradation of textile dye navy blue her by Trichosporon beigelii NCIM-3326, Journal of Hazardous Materials, 166 (2-3), 1421-1428, 2009.
• [31] D’Souza, D.T., Tiwari, R., Sah., A.K, Raghukumar, C., Enhanced production of laccase by a marine fungus during treatment of colored effluents and synthetic dyes, Enzyme and Microbial Technology, 38, 504-511, 2006.
• [32] Sánchez-López, M.I., Vanhulle, S.F., Mertens, V., Guerra, G., Figueroa, S.H., Decock, C., Corbisier, A.M., Penninckx, M.J., Autochthonous white rot fungi from the tropical forest: potential of Cuban strains for dyes and textile industrial effluents decolourisation. African Journal of Biotechnology, 7 (12), 1983-1990, 2008.
• [33] Sing, N.N., Husaini, A., Zulkharnain, A., Roslan, H.A., Decolourisation capabilities of ligninolytic enzymes produced by Marasmius cladophyllus UMAS MS8 on remazol brilliant blue R and other azo dyes, Biomed Research International, 2017, 1-8, 2017.