Effects of FOLFIRINOX Components on Pancreatic Adenocarcinoma Cells

Year 2022, Volume: 12 Issue: 1, 1 - 8, 30.06.2022

Sevde Altuntas Kübra Nur Gökdemir

https://doi.org/10.37094/adyujsci.997309

Abstract

Pancreatic adenocarcinoma is one of the lethal types of cancer worldwide. This study aimed to evaluate the cytotoxic effects of FOLFIRINOX compounds [oxaliplatin (OXA), irinotecan (IRI), fluorouracil (5FU), and leucovorin (LEU)] on a pancreatic adenocarcinoma cell line (PANC-1), both individually and collectively. Cell viability was determined using the colorimetric MTT reagent and apoptosis was assessed using the propidium iodide – Hoechst 33342 staining assay.
Based on cell viability data, 5FU and FOLFIRINOX treatments were more potent against PANC-1 than the other test groups. The apoptotic trend, nevertheless, was not different between them and the control group. Moreover, OXA, LEU, and IRI significantly increase apoptotic cell death. Our findings indicate that 5FU and FOLFIRINOX are comparably effective in reducing the PANC-1 cell viability. In the future, 5FU and FOLFIRINOX may be adapted to local pancreatic adenocarcinoma treatments, but the development of these localized drug release platforms requires further attention as the microenvironment of pancreatic adenocarcinoma inherently contains many unknowns.         

Keywords

FOLFIRINOX, PANC-1 cells, Pancreatic adenocarcinoma, Cytotoxicity, Apoptosis

Supporting Institution

TUBITAK

Project Number

121S050

FOLFIRINOX Bileşenlerinin Pankreas Adenokarsinom Hücreleri Üzerindeki Etkileri

Abstract

Pankreatik adenokarsinoma tüm dünyada en ölümcül kanser türlerinden biridir. Bu çalışma, FOLFIRINOX bileşiklerinin (oksaliplatin (OXA), irinotekan (IRI), fluorourasil (5FU) ve leucovorin (LEU)) tekli ve toplu olarak pankreas adenokarsinom hücre hattı (PANC-1) üzerindeki etkilerini hücre canlılığı ve apoptoz için araştırmayı amaçlamıştır. Hücre canlılığı, MTT reaktifi kullanılarak belirlendi ve apoptoz, propidium iyodür – Hoechst 33342 boyama testi kullanılarak değerlendirildi.
Hücre canlılığı sonuçları, FOLFIRINOX uygulamasının diğer test gruplarına kıyasla en yüksek toksisite oranına sahip olduğunu göstermiştir. Ancak apoptotik eğilim kontrol grubundan farklı bulunmadı. Ek olarak, 5FU tekil uygulamada diğer tekil ilaç uygulamalarına nazaran en yüksek toksisiteyi göstermiştir, fakat apoptotik indeksi kontrol grubundan ve FOLFIRINOX tedavisinden farklı bulunmamıştır. Genel olarak, çalışma sonuçları, tedavi rejiminin pankreas adenokarsinom hücre dizileri üzerinde etkili olduğunu gösterdi. Gelecekte, FOLFIRINOX ve 5FU tedavisi lokal pankreas adenokarsinomu tedavilerine uyarlanabilir, ancak pankreas adenokarsinomunun mikroçevresi, doğası gereği pek çok bilinmeyeni barındırdığından, bu lokalize ilaç salım platformlarının geliştirilmesi daha fazla dikkat gerektirmektedir.

Keywords

FOLFIRINOX; PANC-1 hücreleri; Pankreatik Adenokarsinoma; Sitotoksisite; Apoptoz.

Project Number

121S050

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