Liken ve Orkide Bitkisinden İzole Edilen Aktinobakterilerin Biyoaktivite Özellikleri

Year 2023, Volume: 13 Issue: 1&2, 43 - 58, 31.12.2023

Hilal Ateş , Hilal Ay

https://doi.org/10.37094/adyujsci.1221660

Cited By: 1

Abstract

Aktinobakteriler için seçici bir izolasyon prosedürü kullanılarak bir liken ve orkide türünden toplam 10 aktinobakteri izole edilmiştir. İzolatların antimikrobiyal aktiviteleri Aspergillus niger ATCC 16404, Candida albicans ATCC 10231, Enterococcus faecalis ATCC 29212, Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 700603, Pseudomonas aeruginosa ATCC 27853, Salmonella enterica ATCC 13311, Staphylococcus aureus ATCC 29213 ve Listeria monocytogenes NCTC 5348 patojenlerine karşı değerlendirilmiştir. On izolattan sekizi, en az bir patojene karşı antimikrobiyal aktivite göstermiştir. İzolatları moleküler olarak tanımlamak amacıyla 16S rRNA gen dizi analizi yapılmıştır. 16S rRNA geni karşılaştırmalı analizleri bütün izolatların Streptomyces cinsi üyesi olduklarını ve %98,9 – 100 gen dizi benzerliği taşıdıklarını göstermiştir. İzolatların Streptomyces cinsi içerisindeki filogenetik ilişkileri maksimum olabilirlik ve maksimum sıkılık filogenetik ağaçları ile ortaya çıkarılmıştır. Sonuçlar, likenler ve bitki dokuları gibi henüz çalışılmamış çevresel habitatların önemli biyoaktivite özelliklerine sahip yeni aktinobakteriler için potansiyel bir kaynak olabileceğini göstermektedir.

Keywords

16S rRNA Geni, Aktinobakteriler, Antimikrobiyal Aktivite, Liken, Orkide Yumrusu

Bioactivity Features of Novel Actinobacteria Isolated from Lichen and Orchid Plant

Abstract

A total of 10 actinobacterial strains were isolated from a lichen and an orchid species by employing a selective isolation procedure for actinobacteria. Antimicrobial activities of the isolates were evaluated against Aspergillus niger ATCC 16404, Candida albicans ATCC 10231, Enterococcus faecalis ATCC 29212, Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25922, Klebsiella pneumoniae A TCC 700603, Pseudomonas aeruginosa A TCC 27853, Salmonella enterica ATCC 13311, Staphylococcus aureus ATCC 29213 and Listeria monocytogenes NCTC 5348. Out of the 10 isolates, eight showed antimicrobial activity against at least one pathogen. For molecular identification of the strains, 16S rRNA gene sequence analysis was performed. The pairwise comparison of the 16S rRNA gene sequences of the strains with the databases showed that the strains are members of the genus Streptomyces by sharing 98.9 – 100% gene sequence similarities. Phylogenetic relationships of the strains within the genus Streptomyces were revealed by maximum likelihood and maximum parsimony phylogenetic trees. The results revealed that unexplored environmental habitats like lichens and plant tissues may represent a potential reservoir for novel actinobacteria with promising bioactivity features.

Keywords

16S rRNA Gene, Actinobacteria, Antimicrobial Activity, Lichen, Orchid Tuber

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