Cuscuta campestris tedavisi ile Mide Kanseri Hücrelerinde Apoptozun İndüklenmesi ve Reaktif Oksijen Türlerinin Oluşumu Yoluyla Proliferasyonun Engellenmesi

Year 2021, , 271 - 280, 31.08.2021

Huri Bulut , Ezgi Durmuş , Ebru Hacıosmanoğlu , Kübra Bozali , Hilal Şentürk Abdurrahim Koçyiğit

https://doi.org/10.20492/aeahtd.889902

Abstract

Amaç: Kanser tahrip edici, ölümcül bir hastalıktır ve dünya çapındaki ölüm nedenleri arasında ikinci sırada yer almaktadır. Mide kanseri gibi kanser türleri için farklı tedavi yöntemleri mevcut olsa da çoğu tedavi yöntemleri birçok yan etkiye sahiptir. Çeşitli iyileştirici özelliklere sahip Cuscuta campestris' in kanser hücreleri üzerindeki etkilerinin araştırılması yeni bir alandır ve etkileri tam olarak çalışılmamış ve aydınlatılmamıştır. Bu çalışmada, C. campestris özütünün farklı konsantrasyonlarının mide kanseri hücreleri üzerindeki sitotoksik, genotoksik, apoptotik ve reaktif oksijen türlerinin üretimindeki etkilerini araştırdık.
Gereç ve yöntem: Araştırmamızda, C. campestris için antioksidan, pro-oksidan ve radikal temizleyici aktiviteleri değerlendirildi ve miktar tayini LC-MS / MS yöntemi ile analiz edildi. C. Campestris’ in normal hücrelere kıyasla mide kanseri (AGC) hücreleri üzerindeki seçiciliğini göstermek için insan normal deri fibroblastik (CCD-1079Sk) hücre hattı kullanıldı. Apoptoz belirteçlerinin tayininde akridin oranj / etidyum bromür çift boyama, akış sitometrisi ve western blot metotlarından faydalanıldı. Genotoksik aktivite tayini, Comet analizi ile gerçekleştirildi.
Bulgular: Sonuçlarımız, C. Campestris etanolik özütünün doza bağlı bir şekilde, mide kanseri hücre hattı üzerinde normal hücrelere kıyasla önemli ölçüde daha yüksek sitotoksik etkilere sahip olduğunu göstermiştir. Ayrıca, elde ettiğimiz veriler C. campestris'in mide kanseri hücre hatlarında reaktif oksijen türlerinin oluşumunu arttırdığını ve DNA hasarına neden olduğunu açıkça göstermiştir. Yapılan apoptoz tayin ölçümleri C. campestris' in bölünmüş kaspaz-3, bölünmüş kaspaz-9, bölünmüş PARP ve P-53'ü aktive etmesiyle kanser hücreleri üzerinde apoptotik bir etkiye sahip olduğunu da doğrulamıştır.
Sonuç: Bu çalışmanın sonucu olarak, C. Campestris’in mide karsinomu tedavisinde umut vadeden bir antikanser ajanı olabileceği düşünülmektedir.

Keywords

Cuscuta campestris, mide karsinomu, apoptoz, DNA hasarı, reaktif oksijen türleri

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