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Laktasyon Döneminde Oluşturulan Maternal Hipertiroidiye Bağlı Gelişen Öğrenme Bozukluğuna Selenyumun Etkisi

Year 2022, , 242 - 247, 25.12.2022
https://doi.org/10.46332/aemj.954230

Abstract

Amaç: Hipertiroidi gelişimsel dönemde ve yetişkin dönemde hipokampal-bağımlı öğrenme ve bellek fonksiyonunda olumsuz değişikliklere neden olmaktadır. Bu çalışmada, laktasyon döneminde tiroksin verilen annelerin yavrularında meydana gelen öğrenme bozukluğuna selenyumun iyileştirici etkisi araştırıldı.

Araçlar ve Yöntem: Çalışmada 12 adet gebe sıçan kullanıldı. Gebe sıçanlar rastgele üç gruba (grup başına n=8) ayrıldı: Laktasyon dönemi süresince L-tiroksin (0.2 mg/kg/gün) verilen anne sıçanların erkek yavruları laktasyonel hipertiroidi grubu (LHG)’nu, laktas-yonel hipertiroidi oluşturulduktan sonra 39-60. günler arasında selenyum uygulanan sıçanlar laktasyonel hipertiroidi+selenyum grubu (LH+SeG)’nu, laktasyon dönemi süresince SF uygulanan anne sıçanların yavruları kontrol grubunu (KG) oluşturdu. Uzamsal öğrenme ve bellek fonksiyonu Morris su tankı ile test edildi (her grup için n=16). Tüm ölçümler yavru sıçanların 60-66. gününde yapıldı.

Bulgular: Tek yönlü ANOVA testi ile yapılan post-hoc testi; LHG sıçanların, kontrol grubuna göre daha uzun mesafe kat ederek ve daha uzun süre yüzerek platformu bulduklarını gösterdi (p<0.001). Ayrıca daha yavaş yüzme hızı ve daha yüksek anksiyete bulgusu LHG grubu için diğer bulgulara eşlik etti (p<0.001). LH+SeG sıçanlarda ise kontrol grubundan anlamlı bir farklılık bulunmadı (p>0.005). Probe (bellek testi) testinde hedef kadranda bulunma süresi bakımından gruplar arasında anlamlı farklılık bulunmadı.

Sonuç: Çalışma bulguları, laktasyon döneminde anne sıçanlarda tiroit hormon seviyesi yüksekliğinin, yavru sıçanlarda hipokampal bağımlı öğrenme fonksiyonunda uzun süreli olumsuz etkileri olduğunu ve bu olumsuz etkiler üzerinde selenyumun iyileştirici etkisinin olduğunu göstermektedir. 

References

  • 1. Reis-Lunardelli EAd, Ramirez MR, Castro CC, et al. Effects of an acute treatment with L-thyroxine on memory, habituation, danger avoidance, and on Na+, K+-ATPase activity in rat brain. Curr. Neurovasc. Res. 2007;4(4):259-267.
  • 2. Calloni GW, Penno CA, Cordova FM, et al. Congenital hypothyroidism alters the phosphorylation of ERK1/2 and p38MAPK in the hippocampus of neonatal rats. Dev. Brain Res. 2005;154(1):141-145.
  • 3. Morris RG, Garrud P, Rawlins Ja, et al. Place navigation impaired in rats with hippocampal lesions. Nature. 1982;297(5868):681-683.
  • 4. Anderson P, Morris R, Amaral D, et al. The hippocampus book. United Kingdom, Oxford University Press, 2007.
  • 5. Hashimoto K, Curty FH, Borges PP, et al. An unliganded thyroid hormone receptor causes severe neurological dysfunction. PNAS. 2001;98(7):3998-4003.
  • 6. Gerges NZ, Alzoubi KH, Park CR, et al. Adverse effect of the combination of hypothyroidism and chronic psychosocial stress on hippocampus-dependent memory in rats. Behav. Brain Res. 2004;155(1):77-84.
  • 7. Hosseini M, Dastghaib SS, Rafatpanah H, et al. Nitric oxide contributes to learning and memory deficits observed in hypothyroid rats during neonatal and juvenile growth. Clinics. 2010;65(11):1175-1181.
  • 8. Tan B, Bakkaloğlu U, Aşçıoğlu M, et al. Maternal l-thyroxine treatment during lactation affects learning and anxiety-like behaviors but not spatial memory in adult rat progeny. Pharmacol. Rep. 2021;73(2):454-463.
  • 9. Nicholson JL, Altman J. The effects of early hypo and hyperthyroidism on the development of rat cerebellar cortex. I. Cell proliferation and differentiation. Brain Res. 1972;44(1):13-23.
  • 10. Pavlides C, Westlind-Danielsson A, Nyborg H, et al. Neonatal hyperthyroidism disrupts hippocampal LTP and spatial learning. Exp. Brain Res. 1991;85(3):559-564.
  • 11. Taşkın E, Artis AS, Bitiktas S, et al. Experimentally induced hyperthyroidism disrupts hippocampal longterm potentiation in adult rats. Neuroendocrinology. 2011;94(3):218-227.
  • 12. Meinhold H, Campos-Barros A, Walzog B, et al. Effects of selenium and iodine deficiency on type I, type II and type III iodothyronine deiodinases and circulating thyroid hormones in the rat. Exp. Clin. Endocrinol. 1993;101(2):87-93.
  • 13. Sur Ü, Erkekoğlu P, Koçer-Gümüşel B. Selenyum, Selenoproteinler ve Hashimoto Tiroiditi. Fabad J. Pharm. Sci. 2020;45(1):45-63.
  • 14. Schmutzler C, Mentrup B, Schomburg L, et al. Selenoproteins of the thyroid gland: expression, localization and possible function of glutathione peroxidase 3. Biol. Chem. 2007;388(10):1053-1059.
  • 15. Schomburg L, Köhrle J. On the importance of selenium and iodine metabolism for thyroid hormone biosynthesis and human health. Mol. Nutr. & Food Res. 2008;52(11):1235-1246.
  • 16. Howie A, Walker S, Åkesson B, et al. Thyroidal extracellular glutathione peroxidase: a potential regulator of thyroid-hormone synthesis. Biochem. J. 1995;308(3): 713-717.
  • 17. Abedelhaffez A, Hassan A. Brain derived neurotrophic factor and oxidative stress index in pups with developmental hypothyroidism: neuroprotective effects of selenium. Acta Physiol. Hung. 2013;100(2):197-210.
  • 18. Liu M-C, Xu Y, Chen Y-M, et al. The effect of sodium selenite on lead induced cognitive dysfunction. Neurotoxicology. 2013;36:82-88.
  • 19. Babür E, Tan B, Yousef M, et al. Deficiency but Not Supplementation of Selenium Impairs the Hippocampal Long-Term Potentiation and Hippocampus Dependent Learning. Biol. Trace Elem. Res. 2019;192(2):252-262.
  • 20. Bitiktaş S, Tan B, Batakçı M, et al. Effects of selenium treatment on 6 - n - propyl - 2 - thiouracil - induced impairment of long-term potentiation. Neurosci. Res. 2016;109:70-76.
  • 21. Vara H, Martı́nez B, Santos A, et al. Thyroid hormone regulates neurotransmitter release in neonatal rat hippocampus. Neuroscience. 2002;110(1):19-28.
  • 22. IBM Corp. IBM SPSS Statistics V21. Published online 2012.
  • 23. Vorhees CV, Williams MT. Morris water maze: procedures for assessing spatial and related forms of learning and memory. Nat. Protoc. 2006;1(2):848-858.
  • 24. Zhang L, Hernández VS, Medina‐Pizarro M, et al. Maternal hyperthyroidism in rats impairs stress coping of adult offspring. J. Neurosci. Res. 2008;86(6):1306-1315.
  • 25. Bitiktaş S, Kandemir B, Tan B, et al. Adult-onset hyperthyroidism impairs spatial learning: possible involvement of mitogen-activated protein kinase signaling pathways. Neuroreport. 2016;27(11):802-808.
  • 26. Valverde-R C, Orozco A, Solís-S JC, et al. Iodothyronine deiodinases: emerging clinical crossroads. Cell. Endocrinol. Health and Disease. 2014:365-377.

The Effect of Selenium on Learning Disability Due to Maternal Hyperthyroidism During Lactation

Year 2022, , 242 - 247, 25.12.2022
https://doi.org/10.46332/aemj.954230

Abstract

Purpose: Hyperthyroidism causes adverse changes in hippocampal-dependent learning/memory functions in developmental and adult periods. In the present study, the healing effect of selenium was investigated on learning disability in offspring of mothers given L-thyroxine during lactation.

Materials and Methods: 12 pregnant rats were used in the study. The pregnant rats were randomly assigned into three groups (n=8/group): Male offspring of dams administered L-thyroxine (0.2 mg/kg/day) during the lactation was formed as lactational hyper-thyroidism group (LHG), male offspring of dams administered selenium between 39-60 days after induced lactational hyperthyroid-ism was formed as lactational hyperthyroidism+selenium group (LH+SeG), male offspring of dams administered SF during the lacta-tion period was formed the control group (KG). Spatial learning/memory functions were measured with the Morris water maze (n=16/group). All measurements were made when offspring rats were 60-66 days.

Results: Post-hoc test with one-way ANOVA revealed that LHG rats were found on the platform by traveling longer distances and swimming longer than the control group (p<0.001). In addition, slower swimming speed and higher anxiety findings accompanied other findings for the LHG group (p<0.001). There was not any significant difference between the control group and LH+SeG rats. (p>0.005). There was also not any significant difference between the groups in terms of the time spent in the target quadrant in the probe trial.

Conclusion: The study findings show that high thyroid hormone levels in mother rats during lactation have long-term negative effects on hippocampal-dependent learning function in offspring rats, and selenium has a healing effect on these disorders.

References

  • 1. Reis-Lunardelli EAd, Ramirez MR, Castro CC, et al. Effects of an acute treatment with L-thyroxine on memory, habituation, danger avoidance, and on Na+, K+-ATPase activity in rat brain. Curr. Neurovasc. Res. 2007;4(4):259-267.
  • 2. Calloni GW, Penno CA, Cordova FM, et al. Congenital hypothyroidism alters the phosphorylation of ERK1/2 and p38MAPK in the hippocampus of neonatal rats. Dev. Brain Res. 2005;154(1):141-145.
  • 3. Morris RG, Garrud P, Rawlins Ja, et al. Place navigation impaired in rats with hippocampal lesions. Nature. 1982;297(5868):681-683.
  • 4. Anderson P, Morris R, Amaral D, et al. The hippocampus book. United Kingdom, Oxford University Press, 2007.
  • 5. Hashimoto K, Curty FH, Borges PP, et al. An unliganded thyroid hormone receptor causes severe neurological dysfunction. PNAS. 2001;98(7):3998-4003.
  • 6. Gerges NZ, Alzoubi KH, Park CR, et al. Adverse effect of the combination of hypothyroidism and chronic psychosocial stress on hippocampus-dependent memory in rats. Behav. Brain Res. 2004;155(1):77-84.
  • 7. Hosseini M, Dastghaib SS, Rafatpanah H, et al. Nitric oxide contributes to learning and memory deficits observed in hypothyroid rats during neonatal and juvenile growth. Clinics. 2010;65(11):1175-1181.
  • 8. Tan B, Bakkaloğlu U, Aşçıoğlu M, et al. Maternal l-thyroxine treatment during lactation affects learning and anxiety-like behaviors but not spatial memory in adult rat progeny. Pharmacol. Rep. 2021;73(2):454-463.
  • 9. Nicholson JL, Altman J. The effects of early hypo and hyperthyroidism on the development of rat cerebellar cortex. I. Cell proliferation and differentiation. Brain Res. 1972;44(1):13-23.
  • 10. Pavlides C, Westlind-Danielsson A, Nyborg H, et al. Neonatal hyperthyroidism disrupts hippocampal LTP and spatial learning. Exp. Brain Res. 1991;85(3):559-564.
  • 11. Taşkın E, Artis AS, Bitiktas S, et al. Experimentally induced hyperthyroidism disrupts hippocampal longterm potentiation in adult rats. Neuroendocrinology. 2011;94(3):218-227.
  • 12. Meinhold H, Campos-Barros A, Walzog B, et al. Effects of selenium and iodine deficiency on type I, type II and type III iodothyronine deiodinases and circulating thyroid hormones in the rat. Exp. Clin. Endocrinol. 1993;101(2):87-93.
  • 13. Sur Ü, Erkekoğlu P, Koçer-Gümüşel B. Selenyum, Selenoproteinler ve Hashimoto Tiroiditi. Fabad J. Pharm. Sci. 2020;45(1):45-63.
  • 14. Schmutzler C, Mentrup B, Schomburg L, et al. Selenoproteins of the thyroid gland: expression, localization and possible function of glutathione peroxidase 3. Biol. Chem. 2007;388(10):1053-1059.
  • 15. Schomburg L, Köhrle J. On the importance of selenium and iodine metabolism for thyroid hormone biosynthesis and human health. Mol. Nutr. & Food Res. 2008;52(11):1235-1246.
  • 16. Howie A, Walker S, Åkesson B, et al. Thyroidal extracellular glutathione peroxidase: a potential regulator of thyroid-hormone synthesis. Biochem. J. 1995;308(3): 713-717.
  • 17. Abedelhaffez A, Hassan A. Brain derived neurotrophic factor and oxidative stress index in pups with developmental hypothyroidism: neuroprotective effects of selenium. Acta Physiol. Hung. 2013;100(2):197-210.
  • 18. Liu M-C, Xu Y, Chen Y-M, et al. The effect of sodium selenite on lead induced cognitive dysfunction. Neurotoxicology. 2013;36:82-88.
  • 19. Babür E, Tan B, Yousef M, et al. Deficiency but Not Supplementation of Selenium Impairs the Hippocampal Long-Term Potentiation and Hippocampus Dependent Learning. Biol. Trace Elem. Res. 2019;192(2):252-262.
  • 20. Bitiktaş S, Tan B, Batakçı M, et al. Effects of selenium treatment on 6 - n - propyl - 2 - thiouracil - induced impairment of long-term potentiation. Neurosci. Res. 2016;109:70-76.
  • 21. Vara H, Martı́nez B, Santos A, et al. Thyroid hormone regulates neurotransmitter release in neonatal rat hippocampus. Neuroscience. 2002;110(1):19-28.
  • 22. IBM Corp. IBM SPSS Statistics V21. Published online 2012.
  • 23. Vorhees CV, Williams MT. Morris water maze: procedures for assessing spatial and related forms of learning and memory. Nat. Protoc. 2006;1(2):848-858.
  • 24. Zhang L, Hernández VS, Medina‐Pizarro M, et al. Maternal hyperthyroidism in rats impairs stress coping of adult offspring. J. Neurosci. Res. 2008;86(6):1306-1315.
  • 25. Bitiktaş S, Kandemir B, Tan B, et al. Adult-onset hyperthyroidism impairs spatial learning: possible involvement of mitogen-activated protein kinase signaling pathways. Neuroreport. 2016;27(11):802-808.
  • 26. Valverde-R C, Orozco A, Solís-S JC, et al. Iodothyronine deiodinases: emerging clinical crossroads. Cell. Endocrinol. Health and Disease. 2014:365-377.
There are 26 citations in total.

Details

Primary Language Turkish
Subjects Clinical Sciences
Journal Section Original Articles
Authors

Burak Tan 0000-0003-0782-7759

Ercan Babur 0000-0003-1445-6423

Umut Bakkaloğlu 0000-0002-0136-2279

Ayşenur Çimen This is me 0000-0003-0731-4857

Cem Süer This is me 0000-0002-6455-6644

Meral Aşçıoğlu This is me 0000-0001-8398-4890

Publication Date December 25, 2022
Published in Issue Year 2022

Cite

APA Tan, B., Babur, E., Bakkaloğlu, U., Çimen, A., et al. (2022). Laktasyon Döneminde Oluşturulan Maternal Hipertiroidiye Bağlı Gelişen Öğrenme Bozukluğuna Selenyumun Etkisi. Ahi Evran Medical Journal, 6(3), 242-247. https://doi.org/10.46332/aemj.954230
AMA Tan B, Babur E, Bakkaloğlu U, Çimen A, Süer C, Aşçıoğlu M. Laktasyon Döneminde Oluşturulan Maternal Hipertiroidiye Bağlı Gelişen Öğrenme Bozukluğuna Selenyumun Etkisi. Ahi Evran Med J. December 2022;6(3):242-247. doi:10.46332/aemj.954230
Chicago Tan, Burak, Ercan Babur, Umut Bakkaloğlu, Ayşenur Çimen, Cem Süer, and Meral Aşçıoğlu. “Laktasyon Döneminde Oluşturulan Maternal Hipertiroidiye Bağlı Gelişen Öğrenme Bozukluğuna Selenyumun Etkisi”. Ahi Evran Medical Journal 6, no. 3 (December 2022): 242-47. https://doi.org/10.46332/aemj.954230.
EndNote Tan B, Babur E, Bakkaloğlu U, Çimen A, Süer C, Aşçıoğlu M (December 1, 2022) Laktasyon Döneminde Oluşturulan Maternal Hipertiroidiye Bağlı Gelişen Öğrenme Bozukluğuna Selenyumun Etkisi. Ahi Evran Medical Journal 6 3 242–247.
IEEE B. Tan, E. Babur, U. Bakkaloğlu, A. Çimen, C. Süer, and M. Aşçıoğlu, “Laktasyon Döneminde Oluşturulan Maternal Hipertiroidiye Bağlı Gelişen Öğrenme Bozukluğuna Selenyumun Etkisi”, Ahi Evran Med J, vol. 6, no. 3, pp. 242–247, 2022, doi: 10.46332/aemj.954230.
ISNAD Tan, Burak et al. “Laktasyon Döneminde Oluşturulan Maternal Hipertiroidiye Bağlı Gelişen Öğrenme Bozukluğuna Selenyumun Etkisi”. Ahi Evran Medical Journal 6/3 (December 2022), 242-247. https://doi.org/10.46332/aemj.954230.
JAMA Tan B, Babur E, Bakkaloğlu U, Çimen A, Süer C, Aşçıoğlu M. Laktasyon Döneminde Oluşturulan Maternal Hipertiroidiye Bağlı Gelişen Öğrenme Bozukluğuna Selenyumun Etkisi. Ahi Evran Med J. 2022;6:242–247.
MLA Tan, Burak et al. “Laktasyon Döneminde Oluşturulan Maternal Hipertiroidiye Bağlı Gelişen Öğrenme Bozukluğuna Selenyumun Etkisi”. Ahi Evran Medical Journal, vol. 6, no. 3, 2022, pp. 242-7, doi:10.46332/aemj.954230.
Vancouver Tan B, Babur E, Bakkaloğlu U, Çimen A, Süer C, Aşçıoğlu M. Laktasyon Döneminde Oluşturulan Maternal Hipertiroidiye Bağlı Gelişen Öğrenme Bozukluğuna Selenyumun Etkisi. Ahi Evran Med J. 2022;6(3):242-7.

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