Evaluation of the Therapeutic Effects of Carnosine in Terms of DNA Damage in Diabetic Rat Kidney and Liver Tissues

Year 2022, , 248 - 254, 25.12.2022

Esra Balcıoğlu , Münevver Baran , Fazile Cantürk Tan , Tuba Özkul , Arzu Yay , Menekşe Ülger , Betül Yalçın , Demet Bolat

https://doi.org/10.46332/aemj.991916

Abstract

Purpose: Diabetes mellitus is a metabolic syndrome due to a relative deficiency of insulin secretion or a decrease in the sensitivity of tissues to insulin. It is known that oxidative stress plays a role in the pathophysiology of diabetes-related complications and causes DNA damage. In this study, it was aimed to examine the therapeutic effect of carnosine on DNA damage caused by diabetes on kidney and liver tissues.

Material and Methods: 32 Sprague Dawley rats were divided into 4 groups as control, streptozotocin (STZ), carnosine (10 mg/kg) and STZ+carnosine group. 50 mg/kg STZ was administered intraperitoneally (ip) to 16 rats. On the 3rd day after STZ administration, the blood sugar level of the animals was measured and those above 250 mg/dL were considered as diabetes. Carnosine (10 mg/kg, ip) was administered to 8 of the rats considered to have diabetes for 10 days. Blood glucose levels and body weights were measured at regular intervals. After decapitation, DNA damage was determined by Comet Assay method in obtained liver and kidney tissues.

Results: It was determined that there was a decrease in body weight and an increase in blood glucose levels in the STZ group. According to the results of Comet Assay method, it was determined that there was an increase in DNA damage in kidney and liver tissues of the STZ group, and a decrease in DNA damage in the STZ+carnosine group.

Conclusion: It was concluded that carnosine used as an antioxidant contributes to the reduction of DNA damage caused by diabetes in kidney and liver tissue.

Keywords

Commet Assay, Diabetes mellitus, DNA damage, Streptozotocin, Carnosine

Diyabetik Sıçan Böbrek ve Karaciğer Dokularında Karnozinin Tedavi Edici Etkilerinin DNA Hasarı Açısından Değerlendirilmesi

Abstract

Amaç: Diyabetes mellitus, insülin salgısının göreceli eksikliği veya dokuların insüline duyarlılığındaki azalmaya bağlı meydana gelen metabolik bir sendromdur. Oksidatif stresin diyabete bağlı komplikasyonların patofizyolojisinde rol oynadığı ve DNA hasarına neden olduğu bilinmektedir. Bu çalışmada diyabetin böbrek ve karaciğer dokularında meydana getirdiği DNA hasarına karşı karnozinin tedavi edici etkisinin incelenmesi amaçlanmıştır.

Araçlar ve Yöntem: 32 tane Sprague Dawley cinsi sıçan; kontrol, streptozotosin (STZ), karnozin ve STZ+karnozin grubu olmak üzere 4 gruba ayrıldı. 16 sıçana 50 mg/kg STZ intraperitoneal (ip) olarak uygulandı. STZ uygulamasından sonraki 3. günde hayvanların kan şekeri seviyesi ölçüldü ve 250 mg/dL’nin üzerindekiler diyabet olarak kabul edildi. Diyabetli sıçanlardan 8’ine 10 gün süreyle karnozin uygulandı. Düzenli aralıklarla kan şekeri seviyeleri ve vücut ağırlıkları ölçüldü. Dekapitasyon sonrası elde edilen karaciğer ve böbrek dokularında Comet Assay yöntemi ile DNA hasarı belirlendi.

Bulgular: STZ grubunda vücut ağırlığında azalma ve kan glikoz düzeylerinde artış meydana geldiği tespit edildi. Comet Assay yöntemi sonuçlarına göre STZ grubunun böbrek ve karaciğer dokularında DNA hasarında artış, STZ+karnozin grubunda ise DNA hasarında azalma meydana geldiği belirlendi.

Sonuç: Antioksidan olarak kullanılan karnozinin böbrek ve karaciğer dokusunda diyabetin neden olduğu DNA hasarının azalmasına katkı sağladığı sonucuna varıldı.

Keywords

Commet Assay, DNA hasarı, Streptozotosin, Karnozin, Diyabetes mellitus

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