Sıçanlarda Oluşturulan Deneysel İntesti̇nal İskemi̇ Reperfüzyon Modeli̇nde Querci̇tri̇ni̇n Etki̇leri̇

Year 2018, Volume: 2 Issue: 1, 5 - 10, 17.04.2018

Filiz Yıldız Yunus Dönder , Türkmen Bahadır Arıkan

Abstract

ÖZET

Amaç:Antioksidan özelliği bilinen
quercitrinin deneysel intestinal iskemi reperfüzyon (İ-R) modelinde reperfüzyon
hasarı üzerine etkilerini araştırmak.

Araçlar ve Yöntem : Çalışmada 30 adet
Wistar–Albino sıçan kullanıldı. Sham grubu (n=10), İ-R (kontrol) grubu (n=10)
ve quercitrin+iskemi-reperfüzyon (Q+İ-R) grubu (n=10) olmak üzere 3 grup
oluşturuldu. Sham grubundaki sıçanlar laparotomiyi takiben 3 saat gözlem
sonrası sakrifiye edildi. İ-R grubuna 1 saat iskemi ve takiben 2 saat
reperfüzyon uygulandı. Q+İ-Rgrubuna 24 saat önce gastrik gavaj ile 5 mg/kg
dozunda quercitrin verildi. Histopatolojik inceleme ve doku malondialdehit
(MDA), süperoksiddismutaz (SOD), glutatyonperoksidaz (GSH-Px), nitrik oksit
(NO) ve 8-hidroksi-deoksiguanozin (8-OHDG) aktivitesi ölçümü için ileum
rezeksiyonu yapıldıktan sonra sıçanlar sakrifiye edildi.

Bulgular:Histopatolojik
olarak Q+İ-R ve İ-R grupları arasındaki fark anlamlı bulundu (p<0.001). Q+İ-R
grubunda MDA, NO ve 8-OHDG aktivitesinde azalış istatistiksel olarak anlamlıydı
(p<0,001). GSH-Px ve SOD aktivitesinde İ-R göre daha fazla azalmakta idi ve
sham grubuna göre istatistiksel olarak anlamlıydı. (p<0,001).

Sonuç:Quercitrinin İ-R ile oluşan oksidatif
stresi önlemiş ancak vücudun doğal oksidatif stres koruyucuları üzerinde etki
yapmamıştı.

Keywords

İskemi, reperfüzyon, quercitrin, ince barsak

The Effects Of Quercitrin In Experimental Rat Model Of Intestinal Ischemia-Reperfusion

Abstract

ABSTRACT

Objective: To investigate effects of quercitrin known for its
antioxidant features on experimental model of intestinal ischemia-reperfusion
(I-R).

Material and method: Thirty Wistar-Albino rats were used in the study.
Rats were assigned into 3 groups including sham group (n=30), I-R group (n=30)
and quercitrin plus I-R group (n=10). In the control group, rats were
sacrificed after 3-hours after laparotomy. In ischemia-reperfusion groups, rats
underwent one hour of ischemia followed by 2 hours of reperfusion. In
quercitrin plus reperfusion group, 5 mg/kg quercitrin was given to rats 24
hours before laparotomy by gastric lavage. Rats were sacrificed after resection
of ileum for histopathological examination and measurements of tissue
malonyldialdehyde (MDA), super oxide dismutase (SOD), glutathione peroxidase
(GHS-PX), nitric oxide (NO) and 8-hydroxydeoxyguanosine (8-oHDG) activities.

Results: Significant differences were detected in histopathological examination
between quercitrin plus I-R and I-R groups (p<0.001). MDA, NO and 8-oHDG
activities were significantly decreased in quercitrin plus I-R group
(p<0.001). The extent of decrease in GSH-Px and SOD activities was greater
when compared to I-R group while it was significantly greater when compared to
sham group (p<0.001).

Conclusion: Quercitrin prevented oxidative stress induced by I-R
but had no effect on native defenders against oxidative stress.

Keywords

Ischemia, reperfusion, quercitrin, small intestine

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