Research Article
BibTex RIS Cite

Sıçanlarda Oluşturulan Deneysel İntesti̇nal İskemi̇ Reperfüzyon Modeli̇nde Querci̇tri̇ni̇n Etki̇leri̇

Year 2018, Volume: 2 Issue: 1, 5 - 10, 17.04.2018

Abstract

ÖZET

Amaç:Antioksidan özelliği bilinen
quercitrinin deneysel intestinal iskemi reperfüzyon (İ-R) modelinde reperfüzyon
hasarı üzerine etkilerini araştırmak.

Araçlar ve Yöntem : Çalışmada 30 adet
Wistar–Albino sıçan kullanıldı. Sham grubu (n=10), İ-R (kontrol) grubu (n=10)
ve quercitrin+iskemi-reperfüzyon (Q+İ-R) grubu (n=10) olmak üzere 3 grup
oluşturuldu. Sham grubundaki sıçanlar laparotomiyi takiben 3 saat gözlem
sonrası sakrifiye edildi. İ-R grubuna 1 saat iskemi ve takiben 2 saat
reperfüzyon uygulandı. Q+İ-Rgrubuna 24 saat önce gastrik gavaj ile 5 mg/kg
dozunda quercitrin verildi. Histopatolojik inceleme ve doku malondialdehit
(MDA), süperoksiddismutaz (SOD), glutatyonperoksidaz (GSH-Px), nitrik oksit
(NO) ve 8-hidroksi-deoksiguanozin (8-OHDG) aktivitesi ölçümü için ileum
rezeksiyonu yapıldıktan sonra sıçanlar sakrifiye edildi.

Bulgular:Histopatolojik
olarak Q+İ-R ve İ-R grupları arasındaki fark anlamlı bulundu (p<0.001). Q+İ-R
grubunda MDA, NO ve 8-OHDG aktivitesinde azalış istatistiksel olarak anlamlıydı
(p<0,001). GSH-Px ve SOD aktivitesinde İ-R göre daha fazla azalmakta idi ve
sham grubuna göre istatistiksel olarak anlamlıydı. (p<0,001).







Sonuç:Quercitrinin İ-R ile oluşan oksidatif
stresi önlemiş ancak vücudun doğal oksidatif stres koruyucuları üzerinde etki
yapmamıştı.

References

  • 1. Zimmerman, B.J. and D.N. Granger, Reperfusion injury. Surg Clin North Am, 1992. 72(1): p. 65-83.
  • 2. Wilhelm, J., Metabolic aspects of membrane lipid peroxidation. Acta Univ Carol Med Monogr, 1990. 137: p. 1-53.
  • 3. Jennings, R.B. and K.A. Reimer, The cell biology of acute myocardial ischemia. Annu Rev Med, 1991. 42: p. 225-46.
  • 4. Green, C.J., et al., The importance of iron, calcium and free radicals in reperfusion injury: an overview of studies in ischaemic rabbit kidneys. Free Radic Res Commun, 1989. 7(3-6): p. 255-64.
  • 5. Bilbao, G., et al., Reduction of ischemia-reperfusion injury of the liver by in vivo adenovirus-mediated gene transfer of the antiapoptotic Bcl-2 gene. Ann Surg, 1999. 230(2): p. 185-93.
  • 6. Palmer, R.M., A.G. Ferrige, and S. Moncada, Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor. Nature, 1987. 327(6122): p. 524-6.
  • 7. Ochoa, J.B., et al., Nitrogen oxide levels in patients after trauma and during sepsis. Ann Surg, 1991. 214(5): p. 621-6.
  • 8. Petros, A., D. Bennett, and P. Vallance, Effect of nitric oxide synthase inhibitors on hypotension in patients with septic shock. Lancet, 1991. 338(8782-8783): p. 1557-8.
  • 9. Bird, R.P. and H.H. Draper, Comparative studies on different methods of malonaldehyde determination. Methods Enzymol, 1984. 105: p. 299-305.
  • 10. Harri, M., et al., Analysis of 8-hydroxy-2'-deoxyguanosine in urine using high-performance liquid chromatography-electrospray tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci, 2007. 853(1-2): p. 242-6.
  • 11. Girotti, lipit hydroperoxide generation,turnover and effector action in biological systems. j lipit res, 2000. 39: p. 1529-1542.
  • 12. T,E., kan malondialdehid ve total antioksidan seviyesinin önemi. cerrahi tıp bülteni, 2001. 2: p. 154-167.
  • 13. Halliwell, B., Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? Lancet, 1994. 344(8924): p. 721-4.
  • 14. Haga, S., et al., Hepatic ischemia induced immediate oxidative stress after reperfusion and determined the severity of the reperfusion-induced damage. Antioxid Redox Signal, 2009. 11(10): p. 2563-72.
  • 15. Akkuş, serbest radikaller ve fizyopatolojik etkileri. Mimoza yayınları, 2000: p. 1-124.
  • 16. Nackz, phenolics in cereals, fruits and vegetables: Oceurrence, Extraction and analysis. Journal Pharmaceutical and biomedical analysis, 2006. 21: p. 1523-42.
  • 17. stavric, Role of chemopreventers in human diet. Clin Biochem, 1994. 27: p. 319-332.
  • 18. Formica, Review of the biology of Quercetin and related bioflavonoids. Food Chem Toxicol, 1995. 33(12): p. 1061-80., 1995. 33: p. 1061-80.
  • 19. Bors, Flavonoids as antioxidants: determination of radical-scavenging efficiencies. Methods Enzymol, 1990. 186: p. 343-55.
  • 20. Skaper, Quercetin protects cutaneous tissue-associated cell types including sensory neurons from oxidative stress induced by glutathione depletion: cooperative effects of ascorbic acid. . Free Radic Biol Med, 1997. 22: p. 669-78.
  • 21. Hertog, Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study. Lancet, 1993. 342: p. 1007-11.
  • 22. Cao, Antioxidant and prooxidant behavior of flavonoids: structure-activity relationships. . Free Radic Biol Med, 1997. 22: p. 749-60.
  • 23. grylewski, On the mechanism of antithrombotic action of flavonoids. . Biochem Pharmacol, 1987. 36: p. 317-22.
  • 24. Medina, S.d., Effect of quercitrin on the early stages of hapten induced colonic inflammation in the rat. Life Sci, 2002. 70: p. 3098-108.
  • 25. Hai-dong, sulforafhane protects liver injury induced by intestinal ischemia reperfusion through nrf2-are pathway. world j gastroenterol, 2010. 28: p. 3002-10.
  • 26. Chiu, C.J., et al., Intestinal mucosal lesion in low-flow states. I. A morphological, hemodynamic, and metabolic reappraisal. Arch Surg, 1970. 101(4): p. 478-83.
  • 27. W, T., Ischaemia and infarction. Genel Pathology Seventh Edition, 1996. 709-722.
  • 28. Kazez, the role of melatonin in prevention of intestinal ischemia-reperfusion injury in rats. journal of pediatirc surgery, 2000. 35: p. 1444-1448.
  • 29. Kahraman, Protective effect of quercetin on renal ischemia/reperfusion injury in rats. J Nephrol, 2003. 16: p. 219-24.
  • 30. Schroeter H, S.J.P.E., Rice-Evans C, Current status of the potential role of flavonoids in neuroprotection. Critical reviews of oxidative stress and aging:advances in basic science, diagnostics and intervention. World Scientific Publishing, 2003. 1: p. 137.
  • 31. Camuesco, the intestinal anti-inflammatory effect of quercitrin is associated with an inhibition in iNOS expression. Br J Pharmacol, 2004. 143: p. 908-18.
  • 32. Nijveldt, Flavonoids: a review of probable mechanism of action and potential applications. Clin. Nutr, 2001. 74: p. 418-425.
  • 33. Elliott, The effects of plant flavonoids on mammalian cells: Đmplications for inflammation, heart disease, and cancer. Pharmacological Reviews, 2000. 52: p. 673-751.
  • 34. Priya, Protective effect of quercetin in cisplatininduced cell injury in the rat kidney. Indian J. Pharmacol, 1999. 31: p. 422-426.
  • 35. Nakagawa, Differential effects of flavonoid quercetin on oxidative damages induced by hydrophilic and lipophilic radical generator in hepatic Iysosomal fractions of mice. J. Health Science, 2000. 46: p. 509-512.
  • 36. Halliwell, B., Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? Lancet, 1994. 344(8924): p. 721-4.
  • 37. Shoenberg MH., B.H., Reperfusion injury after intestinal ischemia. Crit Care Med., 1993. 21: p. 1376-1386.
  • 38. Masella, R., et al., Novel mechanisms of natural antioxidant compounds in biological systems: involvement of glutathione and glutathione-related enzymes. J Nutr Biochem, 2005. 16(10): p. 577-86.
  • 39. Halliwell , B., Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? Lancet, 1994. 344(8924): p. 721-4.
  • 40. Kalpaklıoğlu, nitrik oksit. MN, 1996. 4/2: p. 88-90.
  • 41. Shoskes, D.A., Effect of bioflavonoids quercetin and curcumin on ischemic renal injury: a new class of renoprotective agents. Transplantation, 1998. 66: p. 147-52.
  • 42. Huk, I., Bioflavonoid quercetin scavenges superoxide and increases nitric oxide concentration in ischaemia-reperfusion injury: an experimental study. Br J Surg, 1998. 85: p. 1080-5.

The Effects Of Quercitrin In Experimental Rat Model Of Intestinal Ischemia-Reperfusion

Year 2018, Volume: 2 Issue: 1, 5 - 10, 17.04.2018

Abstract

ABSTRACT

Objective: To investigate effects of quercitrin known for its
antioxidant features on experimental model of intestinal ischemia-reperfusion
(I-R).



Material and method: Thirty Wistar-Albino rats were used in the study.
Rats were assigned into 3 groups including sham group (n=30), I-R group (n=30)
and quercitrin plus I-R group (n=10). In the control group, rats were
sacrificed after 3-hours after laparotomy. In ischemia-reperfusion groups, rats
underwent one hour of ischemia followed by 2 hours of reperfusion. In
quercitrin plus reperfusion group, 5 mg/kg quercitrin was given to rats 24
hours before laparotomy by gastric lavage. Rats were sacrificed after resection
of ileum for histopathological examination and measurements of tissue
malonyldialdehyde (MDA), super oxide dismutase (SOD), glutathione peroxidase
(GHS-PX), nitric oxide (NO) and 8-hydroxydeoxyguanosine (8-oHDG) activities.



Results: Significant differences were detected in histopathological examination
between quercitrin plus I-R and I-R groups (p<0.001). MDA, NO and 8-oHDG
activities were significantly decreased in quercitrin plus I-R group
(p<0.001). The extent of decrease in GSH-Px and SOD activities was greater
when compared to I-R group while it was significantly greater when compared to
sham group (p<0.001).



Conclusion: Quercitrin prevented oxidative stress induced by I-R
but had no effect on native defenders against oxidative stress.

References

  • 1. Zimmerman, B.J. and D.N. Granger, Reperfusion injury. Surg Clin North Am, 1992. 72(1): p. 65-83.
  • 2. Wilhelm, J., Metabolic aspects of membrane lipid peroxidation. Acta Univ Carol Med Monogr, 1990. 137: p. 1-53.
  • 3. Jennings, R.B. and K.A. Reimer, The cell biology of acute myocardial ischemia. Annu Rev Med, 1991. 42: p. 225-46.
  • 4. Green, C.J., et al., The importance of iron, calcium and free radicals in reperfusion injury: an overview of studies in ischaemic rabbit kidneys. Free Radic Res Commun, 1989. 7(3-6): p. 255-64.
  • 5. Bilbao, G., et al., Reduction of ischemia-reperfusion injury of the liver by in vivo adenovirus-mediated gene transfer of the antiapoptotic Bcl-2 gene. Ann Surg, 1999. 230(2): p. 185-93.
  • 6. Palmer, R.M., A.G. Ferrige, and S. Moncada, Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor. Nature, 1987. 327(6122): p. 524-6.
  • 7. Ochoa, J.B., et al., Nitrogen oxide levels in patients after trauma and during sepsis. Ann Surg, 1991. 214(5): p. 621-6.
  • 8. Petros, A., D. Bennett, and P. Vallance, Effect of nitric oxide synthase inhibitors on hypotension in patients with septic shock. Lancet, 1991. 338(8782-8783): p. 1557-8.
  • 9. Bird, R.P. and H.H. Draper, Comparative studies on different methods of malonaldehyde determination. Methods Enzymol, 1984. 105: p. 299-305.
  • 10. Harri, M., et al., Analysis of 8-hydroxy-2'-deoxyguanosine in urine using high-performance liquid chromatography-electrospray tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci, 2007. 853(1-2): p. 242-6.
  • 11. Girotti, lipit hydroperoxide generation,turnover and effector action in biological systems. j lipit res, 2000. 39: p. 1529-1542.
  • 12. T,E., kan malondialdehid ve total antioksidan seviyesinin önemi. cerrahi tıp bülteni, 2001. 2: p. 154-167.
  • 13. Halliwell, B., Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? Lancet, 1994. 344(8924): p. 721-4.
  • 14. Haga, S., et al., Hepatic ischemia induced immediate oxidative stress after reperfusion and determined the severity of the reperfusion-induced damage. Antioxid Redox Signal, 2009. 11(10): p. 2563-72.
  • 15. Akkuş, serbest radikaller ve fizyopatolojik etkileri. Mimoza yayınları, 2000: p. 1-124.
  • 16. Nackz, phenolics in cereals, fruits and vegetables: Oceurrence, Extraction and analysis. Journal Pharmaceutical and biomedical analysis, 2006. 21: p. 1523-42.
  • 17. stavric, Role of chemopreventers in human diet. Clin Biochem, 1994. 27: p. 319-332.
  • 18. Formica, Review of the biology of Quercetin and related bioflavonoids. Food Chem Toxicol, 1995. 33(12): p. 1061-80., 1995. 33: p. 1061-80.
  • 19. Bors, Flavonoids as antioxidants: determination of radical-scavenging efficiencies. Methods Enzymol, 1990. 186: p. 343-55.
  • 20. Skaper, Quercetin protects cutaneous tissue-associated cell types including sensory neurons from oxidative stress induced by glutathione depletion: cooperative effects of ascorbic acid. . Free Radic Biol Med, 1997. 22: p. 669-78.
  • 21. Hertog, Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study. Lancet, 1993. 342: p. 1007-11.
  • 22. Cao, Antioxidant and prooxidant behavior of flavonoids: structure-activity relationships. . Free Radic Biol Med, 1997. 22: p. 749-60.
  • 23. grylewski, On the mechanism of antithrombotic action of flavonoids. . Biochem Pharmacol, 1987. 36: p. 317-22.
  • 24. Medina, S.d., Effect of quercitrin on the early stages of hapten induced colonic inflammation in the rat. Life Sci, 2002. 70: p. 3098-108.
  • 25. Hai-dong, sulforafhane protects liver injury induced by intestinal ischemia reperfusion through nrf2-are pathway. world j gastroenterol, 2010. 28: p. 3002-10.
  • 26. Chiu, C.J., et al., Intestinal mucosal lesion in low-flow states. I. A morphological, hemodynamic, and metabolic reappraisal. Arch Surg, 1970. 101(4): p. 478-83.
  • 27. W, T., Ischaemia and infarction. Genel Pathology Seventh Edition, 1996. 709-722.
  • 28. Kazez, the role of melatonin in prevention of intestinal ischemia-reperfusion injury in rats. journal of pediatirc surgery, 2000. 35: p. 1444-1448.
  • 29. Kahraman, Protective effect of quercetin on renal ischemia/reperfusion injury in rats. J Nephrol, 2003. 16: p. 219-24.
  • 30. Schroeter H, S.J.P.E., Rice-Evans C, Current status of the potential role of flavonoids in neuroprotection. Critical reviews of oxidative stress and aging:advances in basic science, diagnostics and intervention. World Scientific Publishing, 2003. 1: p. 137.
  • 31. Camuesco, the intestinal anti-inflammatory effect of quercitrin is associated with an inhibition in iNOS expression. Br J Pharmacol, 2004. 143: p. 908-18.
  • 32. Nijveldt, Flavonoids: a review of probable mechanism of action and potential applications. Clin. Nutr, 2001. 74: p. 418-425.
  • 33. Elliott, The effects of plant flavonoids on mammalian cells: Đmplications for inflammation, heart disease, and cancer. Pharmacological Reviews, 2000. 52: p. 673-751.
  • 34. Priya, Protective effect of quercetin in cisplatininduced cell injury in the rat kidney. Indian J. Pharmacol, 1999. 31: p. 422-426.
  • 35. Nakagawa, Differential effects of flavonoid quercetin on oxidative damages induced by hydrophilic and lipophilic radical generator in hepatic Iysosomal fractions of mice. J. Health Science, 2000. 46: p. 509-512.
  • 36. Halliwell, B., Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? Lancet, 1994. 344(8924): p. 721-4.
  • 37. Shoenberg MH., B.H., Reperfusion injury after intestinal ischemia. Crit Care Med., 1993. 21: p. 1376-1386.
  • 38. Masella, R., et al., Novel mechanisms of natural antioxidant compounds in biological systems: involvement of glutathione and glutathione-related enzymes. J Nutr Biochem, 2005. 16(10): p. 577-86.
  • 39. Halliwell , B., Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? Lancet, 1994. 344(8924): p. 721-4.
  • 40. Kalpaklıoğlu, nitrik oksit. MN, 1996. 4/2: p. 88-90.
  • 41. Shoskes, D.A., Effect of bioflavonoids quercetin and curcumin on ischemic renal injury: a new class of renoprotective agents. Transplantation, 1998. 66: p. 147-52.
  • 42. Huk, I., Bioflavonoid quercetin scavenges superoxide and increases nitric oxide concentration in ischaemia-reperfusion injury: an experimental study. Br J Surg, 1998. 85: p. 1080-5.
There are 42 citations in total.

Details

Primary Language Turkish
Subjects Clinical Sciences
Journal Section Articles
Authors

Filiz Yıldız This is me

Yunus Dönder

Türkmen Bahadır Arıkan This is me

Publication Date April 17, 2018
Published in Issue Year 2018 Volume: 2 Issue: 1

Cite

APA Yıldız, F., Dönder, Y., & Arıkan, T. B. (2018). Sıçanlarda Oluşturulan Deneysel İntesti̇nal İskemi̇ Reperfüzyon Modeli̇nde Querci̇tri̇ni̇n Etki̇leri̇. Ahi Evran Medical Journal, 2(1), 5-10.
AMA Yıldız F, Dönder Y, Arıkan TB. Sıçanlarda Oluşturulan Deneysel İntesti̇nal İskemi̇ Reperfüzyon Modeli̇nde Querci̇tri̇ni̇n Etki̇leri̇. Ahi Evran Med J. April 2018;2(1):5-10.
Chicago Yıldız, Filiz, Yunus Dönder, and Türkmen Bahadır Arıkan. “Sıçanlarda Oluşturulan Deneysel İntesti̇nal İskemi̇ Reperfüzyon Modeli̇nde Querci̇tri̇ni̇n Etki̇leri̇”. Ahi Evran Medical Journal 2, no. 1 (April 2018): 5-10.
EndNote Yıldız F, Dönder Y, Arıkan TB (April 1, 2018) Sıçanlarda Oluşturulan Deneysel İntesti̇nal İskemi̇ Reperfüzyon Modeli̇nde Querci̇tri̇ni̇n Etki̇leri̇. Ahi Evran Medical Journal 2 1 5–10.
IEEE F. Yıldız, Y. Dönder, and T. B. Arıkan, “Sıçanlarda Oluşturulan Deneysel İntesti̇nal İskemi̇ Reperfüzyon Modeli̇nde Querci̇tri̇ni̇n Etki̇leri̇”, Ahi Evran Med J, vol. 2, no. 1, pp. 5–10, 2018.
ISNAD Yıldız, Filiz et al. “Sıçanlarda Oluşturulan Deneysel İntesti̇nal İskemi̇ Reperfüzyon Modeli̇nde Querci̇tri̇ni̇n Etki̇leri̇”. Ahi Evran Medical Journal 2/1 (April 2018), 5-10.
JAMA Yıldız F, Dönder Y, Arıkan TB. Sıçanlarda Oluşturulan Deneysel İntesti̇nal İskemi̇ Reperfüzyon Modeli̇nde Querci̇tri̇ni̇n Etki̇leri̇. Ahi Evran Med J. 2018;2:5–10.
MLA Yıldız, Filiz et al. “Sıçanlarda Oluşturulan Deneysel İntesti̇nal İskemi̇ Reperfüzyon Modeli̇nde Querci̇tri̇ni̇n Etki̇leri̇”. Ahi Evran Medical Journal, vol. 2, no. 1, 2018, pp. 5-10.
Vancouver Yıldız F, Dönder Y, Arıkan TB. Sıçanlarda Oluşturulan Deneysel İntesti̇nal İskemi̇ Reperfüzyon Modeli̇nde Querci̇tri̇ni̇n Etki̇leri̇. Ahi Evran Med J. 2018;2(1):5-10.

Ahi Evran Medical Journal  is indexed in ULAKBIM TR Index, Turkish Medline, DOAJ, Index Copernicus, EBSCO and Turkey Citation Index. Ahi Evran Medical Journal is periodical scientific publication. Can not be cited without reference. Responsibility of the articles belong to the authors.

    Creative Commons Lisansı

This journal is licensed under the Creative Commons Atıf-GayriTicari 4.0 Uluslararası Lisansı.