Farelerde Pentilentetrazol ile Oluşturulan Epileptik Nöbetler Üzerine Anakinra’nın Etkisinin Araştırılması

Year 2021, Volume: 5 Issue: 2, 103 - 108, 25.08.2021

Ahmet Kemal Filiz Sebahattin Karabulut

https://doi.org/10.46332/aemj.796322

Abstract

Amaç: Beyinde gama aminobutirik asit (GABA) ve glutamat arasındaki dengenin bozulması, nöbet oluşumuna ve epileptogeneze katkı sağlayan önemli faktörlerden biridir. Bu çalışmanın amacı, anakinra ön tedavisinin pentilentetrazol (PTZ) ile oluşturulan nöbet modelinde kortikal ve hipokampal GABA ve glutamat seviyeleri üzerine etkisinin olup olmadığını belirlemektir.

Araçlar ve Yöntem: Çalışmada 18 adet BALB-c türü fare Kontrol, PTZ ve Anakinra grupları şeklinde 3 gruba ayrıldı. Nöbetleri başlatmak için 60 mg/kg dozda PTZ enjeksiyonu farelere intraperitonal olarak uygulandı. Anakinra grubuna PTZ enjeksiyonundan 30 dakika önce intraperitonal olarak anakinra (100 mg/kg) uygulandı. Hayvanların kortikal ve hipokampal GABA ve glutamat düzeyleri Enzyme Linked Immunosorbent Assay (ELISA) yöntemi kullanılarak ölçüldü.

Bulgular: PTZ'nin neden olduğu nöbetler, hipokampusta glutamat seviyesini artırırken (p<0.001), GABA düzeyinde azalmaya yol açtı (p<0.05). PTZ tedavisi kortikal glutamat seviyesini arttırdı (p<0.05). Anakinra ile ön tedavi hipokampusta glutamat düzeyini azaltırken (p<0.001), GABA düzeyinde artışa yol açtı (p<0.01). Ayrıca anakinra ön tedavisi kortikal glutamat seviyesini azaltırken (p<0.05), GABA düzeyinde artışla sonuçlandı (p<0.001).

Sonuç: PTZ'nin neden olduğu nöbetler beyinde GABA düzeyinin azalmasına ve glutamat seviyesinin artışına neden olmaktadır. PTZ enjeksiyonu öncesinde anakinra tedavisi farelerin beyinlerinde uyarılma lehine bozulmuş GABA/Glutamat dengesizliğini iyileştir-mektedir.

Keywords

GABA, Glutamat, PTZ, Anakinra

Supporting Institution

Herhangi bir kurumdan destek alınmamıştır.

Investigation of the Effect of Anakinra on Pentylenetetrazole Induced Epileptic Seizures in Mice

Abstract

Purpose: Disruption of the balance between gamma aminobutyric acid (GABA) and glutamate in the brain is one of the important factors contributing to seizure formation and epileptogenesis. The aim of this study is to determine whether anakinra pretreatment has an effect on cortical and hippocampal GABA and glutamate levels in a seizure model induced with pentylenetetrazole (PTZ).

Materials and Methods: In the study, 18 BALB-c type mice were divided into 3 groups as Control, PTZ, and Anakinra groups. An injection of 60 mg/kg PTZ was administered intraperitoneally to mice to induce seizures. Anakinra group was administered intraperitoneally (100 mg/kg) 30 minutes before PTZ injection. The cortical and hippocampal GABA and glutamate levels of the animals were measured using the Enzyme Linked Immunosorbent Assay (ELISA) method.

Results: PTZ-induced seizures led to a decrease in the hippocampal GABA level (p<0.05), while the glutamate level increased (p<0.001). The treatment of PTZ also increased the level of cortical glutamate (p<0.05). The pretreatment with anakinra led to a decrease in the hippocampal glutamate level (p<0.001), but increased GABA level (p<0.01). In addition, the pretreatment with anakinra cause to decrease the level of cortical glutamate (p<0.05), while the GABA level increased (p<0.001).

Conclusion: PTZ-induced seizures cause a decrease in GABA levels and an increase in glutamate levels in the brain. Anakinra treatment prior to PTZ injection ameliorates the GABA/Glutamate imbalance that has shifted to the excitation side in the brains of mice. 

Keywords

GABA, Glutamat, PTZ, Anakinra

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