Karyotype analysis of Astragalus stenosemioides (Fabaceae)

Abstract

In this research the metaphase chromosome number and karyotype of Astragalus stenosemioides species growing naturally in Turkey are studied. The study revealed that the chromosome number of A. stenosemioides is 2n = 16. The basic chromosome number of species is determined as x = 8. The karyotype formula of A. stenosemioides is 8m. The total length of the somatic chromosomes of A. stenosemioides ranges between 1.96 - 3.38 μm. The total haploid chromosome length of A. stenosemioides is determined as 21.36 μm. In addition, the karyotype asymmetry of the species was evaluated by different methods; Stebbins classification, TF%, AsK%, Syi and Rec, A1, A2, DI, A, and AI.

Keywords

• Astragalus
• Fabaceae
• Karyotype
• Turkey

Project Number

FDK-2015-5693

Astragalus stenosemioides (Fabaceae)’in karyotip analizi

Abstract

Bu çalışmada Türkiye'de doğal olarak yetişen Astragalus stenosemioides türünün metafaz kromozom sayısı ve karyotipi incelenmiştir. Çalışmada A. stenosemioides'in kromozom sayısının 2n = 16 olduğu tespit edilmiştir. Türün temel kromozom sayısı x = 8 olarak belirlenmiştir. A. stenosemioides'in karyotip formülü 8m'dir. Astragalus stenosemioides'in somatik kromozomlarının toplam uzunluğu 1.96 - 3.38 µm arasında değişmektedir. A. stenosemioides'in toplam haploid kromozom uzunluğu 21.36 µm olarak belirlenmiştir Ayrıca türün karyotip asimetrisi; Stebbins sınıflandırması, %TF, %AsK, Syi ve Rec, A1, A2, DI, A ve AI gibi farklı yöntemlerle değerlendirilmiştir.

Keywords

• Astragalus
• Fabaceae
• Karyotip
• Türkiye

Supporting Institution

Erciyes Üniversitesi

Project Number

FDK-2015-5693

References

1. Arano H, Saito H (1980). Cytological studies in family Umbelliferae 5. Karyotypes of seven species in subtribe Seselinae. La Kromosomo 2: 471-480.
2. Arano H (1963). Cytological studies in subfamily Carduoideae (Compositae) of Japan. IX. The karyotype analysis and phylogenic considerations on Pertya and Ainsliaea. Botanical Magazine (Tokyo) 76: 32-39.
3. Aryavand A (1977). IOPB chromosome number reports LVII. Taxon 26: 443-452.
4. Astanova SB (1981). Chromosome numbers of Leguminosae of flora in Tajikistan, Doklady Akademii Nauk SSSR 24: 61-63.
5. Atasagun B, Aksoy A, Martin E (2018). Morphological, anatomical, palynological, karyological and ecological remarks of Astragalus argaeus (Fabaceae) endemic to Turkey. Phytotaxa 379 (1): 118-130.
6. Aytaç Z (1997). The revision of the section Dasyphyllium Bunge of the genus Astragalus L. of Turkey. Turkish Journal of Botany 21: 31-59.
7. Borjian A, Assadi M, Nejadsattari T, Maassoumi AA (2012). Based on cytotaxonomical and morphological evidence Astragalus remotiflorus subsp. nigrohirsutus is raised to species Rank. Iranian Journal of Botany 18(1): 98-103.
8. Cartier D (1979). Premières prospections caryologiques du genre Astragalus L. dans la flore du bassin méditerranéen oriental. Revue de Cytologie et de Biologie Végétales, le Botaniste 2:169-181.

9. Davina JR, Gomez-Sosa E (1993). Cariotipo de siete especies del genero Astragalus (Leguminosae) de la Argentina. Boletin de la Sociedad Argentina de Botanica 29: 197-201.
10. Dawe JC, Murray DF (1979). IOPB chromosome number reports LXIII. Taxon 28: 265-268.
11. Ekici M, Ekim T (2004). Revision of the section Hololeuce Bunge of the genus Astragalus L. (Leguminosae) in Turkey. Turkish Journal of Botany 28(3): 307-347.
12. Ekici M, Çelik S (2005). Autoecological and morphological features of Astragalus stenosemioides DF Chamb and VA Matthews. Journal of Biological Sciences 5 (5): 553-557.
13. Ekici M, Yüzbaşıoğlu D, Aytaç Z (2005). Morphology, pollen, seed structure and karyological study on Astragalus ovalis Boiss. & Balansa (Sect. Ammodendron) in Turkey. International Journal of Botany 1(1): 74-78.
14. Gervais C, Trahan R, Gagnon J (1999). IOPB chromosome data 14. Newslett. Int. Organ. Plant Biosystems (Oslo) 30: 10-15.
15. Greilhuber J, Speta F (1976). C-banded karyotypes in the Scilla hohenackeri Group, S. persica and Puschkinia (Liliaceae). Plant Systematic Evolution 126: 149-188.
16. Hamzaoğlu E (2020). Astragalus askaleensis (sect.Adiaspastus, Fabaceae), a new species from Turkey. Türler ve Habitatlar 1(2): 114-123.
17. Hung LY (1984). Karyotype Analysis of 5 species of Genus Astragalus. Acta Phytotaxonomica Sinica 22(2): 125-127.
18. Huziwara Y (1962). Karyotype analysis in some genera of Compositae. VIII. Further studies on the chromosome of Aster. American Journal of Botany 49: 116-119.İlçim A, Behçet L (2016). Astragalus topalanense (Fabaceae), a new species from Turkey. Turkish Journal of Botany 40: 74-80.
19. Kandemir N, Korkmaz H, Engin A (1996). The morphological and anatomical properties of Astragalus barba-jovis DC. var. barba-jovis (Fabaceae). Turkish Journal of Botany 20: 291-299.
20. Karaman Erkul S, Aytaç Z, Ekici M (2016). Synopsis of the sect. Hymenocoleus, sect. Hymenostegis, and sect. Macrophyllium belonging to Astragalus (Fabaceae) in Turkey. Turkish Journal of Botany 40: 412-418.
21. Karaman Erkul S, Bagheri A, Maassoumi AA, Rahiminejad MR (2014). Notes on Astragalus sect. Hymenostegis (Fabaceae) from Turkey. Turkish Journal of Botany 39: 205-207.
22. Kazem Y, Houshmand S, Dadane GZ (2010). Karyotype analysis of Astragalus effusus Bunge (Fabaceae). Caryologia 63(3): 257-261.
23. Lavania UC, Srivastava S (1992). A simple parameter of dispersion index that serves as a adjunct to karyotype asymmetry. Journal of Biosciences 17: 179-182.
24. Liston A (1990). Chromosome counts in Astragalus (Fabaceae). Madroño 37: 59-60.
25. Maassoumi AA (1987). Notes on the genus Astragalus in Iran I, cytotaxonomic sudies on some species, The Iranian Journal of Botany 3: 117-128.
26. Maassoumi AA (1989). The genus Astragalus in Iran. Research Institute of Forests and Rangelands Technical Publication 2: 257-386.
27. Maassoumi AA (1998). Astragalus in the Old World Check-List. Tehran: Islamic Reublic of Iran, Ministry of Jahad-e-Sazandegi, Research Institute of Forests and Rangelands.
28. Magulaev AJ (1980). Chromosome numbers of some Fabaceae in the North Caucasus. Botanicheskii Zhurnal 65(6): 836-843.
29. Magulaev AJ (1989). Chromosome numbers in some Astragalus (Fabaceae) species of the Caucasus flora. Botanicheskii Zhurnal (Moscow and Leningrad) 74: 1519-1521.
30. Martin E, Doğan İçyer G, Karaman Erkul S, Eroğlu HE (2019). Karyotype analyses of 25 Turkish taxa of Astragalus from section Macrophyllium, Hymenostegis, Hymenocoleus and Anthylloidei (Fabaceae). Turkish Journal of Botany 43(1): 232-242.
31. Martin E, Duran A, Dinç M, Erişen S (2008). Karyotype analyses of four Astragalus L. (Fabaceae) species from Turkey. Phytologia 90(2): 147-159.
32. Mu SM, Shue LZ (1985). Chromosome Number Reports LXXXIX. Taxon 34: 27-730.
33. Murray DF, Kelso S. (1997). Chromosome numbers and notes on the taxonomy of selected Alaskan vascular plants. Rhodora 99: 33-55.
34. Nazarova EA (2004). Chromosome numbers of flowering plants of Armenian Flora, Yerevan: 1-171.
35. Ranjbar M, Assadi A, Karamian R (2012). Notes on Astragalus sect. Macrophyllium with a cytogenetic report on its two tetraploid species. Journal of Agricultural Science and Technology 11(2): 211-226.
36. Sheidai M, Gharemaninejad F (2009). New chromosome number and karyotype analysis in four Astragalus L. (Fabaceae) species. Iranian Journal of Botany 15(1): 21-26.
37. Sheidai M, Shahin Z, Jalal I (2009). New chromosome number reports in tragacanthic Astragalus species. Caryologia 62(1): 30-36.
38. Spellenberg R (1981). Chromosome numbers and their cytotaxonomic significance for North American Astragalus (Fabaceae). Taxon 25: 463-472.
39. Stebbins GL (1971). Chromosomal evolution in higher plants. London: Edward Arnold.
40. Ward DE, Spellenberg R (1988). Chromosome counts of angiosperms from New Mexico and adjacent areas. Phytologia 64: 390-398.
41. Watanabe K, Yahara T, Denda T, Kosuge K (1999). Chromosomal evolution in the genus Brachyscome (Asteraceae, Astereae): Statistical tests regarding correlation between changes in karyotype and habit using phylogenetic information. Journal of Plant Research 112: 145-161.
42. Yan GX, Zhang SZ, Xue FH, Wang LY, Yun JF, Fu XQ (2000). The chromosome numbers and natural distribution of 38 forage plants in north China. Grassland of China 5: 1-5.
43. Zarco RC (1986) A new method for estimating karyotype asymmetry. Taxon 35: 526-530.