Protective effects of resveratrol on permethrin-induced fetotoxicity in rats

Abstract

Synthetic pyrethroid insecticides have been widely used for years to prevent harmful effects of insects and control disease vectors. In this study, the effects of resveratrol against the potential toxicity of permethrin, an effective pyrethroid derivative, on the fetus were investigated. Accordingly, Wistar female rats were divided into four groups as Control, Sham, Permethrin, and Permethrin + Resveratrol. Lung, liver, kidney and small intestine of developing fetuses were evaluated histopathologically. Also, Bone Morphogenetic Protein-4 (BMP-4) in bone tissue development and Fibroblast Growth Factor-1 (FGF-1) expressions in lung were examined immunohistochemically. All structures in the Control and Sham groups were normal. Permethrin caused epithelial damage, regression in bronchial and primitive alveolar development in the lung; congestion, edema and sinusoidal dilatation around the central vein in the liver; tubular epithelial degeneration, regression in glomeruli and tubule formation in the kidney; epithelial degeneration and irregularity in the villus structure in the small intestine. Immunohistochemical results indicated that permethrin administration decreased BMP-4 levels in bone tissue and FGF-1 levels in lung. Resveratrol application was found to greatly alleviate histopathological and immunohistopathological variability in all tissues. Oral consumption of permethrin by pregnant rats caused growth retardation and tissue damage in many different tissues in offspring. Intake of resveratrol during pregnancy showed protective effects against fetotoxicity caused by permethrin.

Keywords

• Permethrin
• Resveratrol
• Fetotoxicity
• FGF-1
• BMP-4

Sıçanlarda permetrin kaynaklı fetotoksisite üzerine resveratrolün koruyucu etkileri

Abstract

Sentetik piretroid insektisitler, yıllardır böceklerin zararlı etkilerinden korunmak ve hastalık vektörlerinin kontrolü için yaygın olarak kullanılmaktadır. Bu çalışmada, etkili bir piretroid türevi olan permetrinin fetüs üzerindeki olası toksisitesine karşı polifenolik bir bileşik olan resveratrolün potansiyel etkileri araştırılmıştır. Çalışmada Wistar dişi sıçanlar Kontrol, Sham, Permetrin ve Permetrin+Resveratrol olmak üzere dört gruba ayrıldı. Histopatolojik olarak fetüslerin akciğer, karaciğer, böbrek ve ince bağırsak gelişimleri değerlendirildi. Ayrıca immunohistokimyasal olarak kemik doku gelişiminde Bone Morphogenetic Protein 4 (BMP-4) ve akciğerde Fibroblast Growth Factor-1 (FGF-1) ekspresyonlarına bakıldı. Kontrol ve Sham grubunda tüm yapılar normal görünümde izlendi. Permetrin; akciğerde epitelyal hasara, bronşiyal ve primitif alveolar gelişimde gerilemeye; karaciğerde santral ven çevresinde konjesyon, ödem ve sinüzoidal dilatasyona; böbrekte tübül epitelinde dejenerasyona, glomerül ve tübül oluşumunda gerilemeye; ince bağırsakta da epitelyal dejenerasyona ve villüs yapısında düzensizliğe neden olduğu saptanmıştır. İmmünohistokimyasal sonuçlarda permetrin uygulaması ile kemik dokuda BMP-4 ve akciğerde FGF-1 ekspresyonlarında azalma saptanmıştır. Resveratrol uygulaması sonrasında tüm dokularda histopatolojik ve immünohistokimyasal değişiklikler büyük oranda hafiflemiştir. Gebe sıçanların oral yolla permetrin tüketimi, yavrularda birçok farklı dokuda gelişme geriliği ve doku hasarına neden olmaktadır. Gebelik sürecinde resveratrol alımı ise permetrinin neden olduğu fetotoksisiteye karşı koruyucu etkinlik göstermektedir.

Keywords

• Permetrin
• Resveratrol
• Fetotoksisite
• FGF-1
• BMP-4

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