Phytophthora capsici’ye maruz bırakılan biber fidelerinin köklerinde nitrat içeriği

Year 2023, , 122 - 127, 15.11.2023

Esra Koç , Belgizar Karayiğit

https://doi.org/10.30616/ajb.1287442

Abstract

Phytophthora capsici ölümcül bir bitki hastalığı olan kök çürüklüğüne neden olur. Hastalıklara karşı direnç, birçok savunma maddesinin aktivasyonu ile üretilir, dolayısıyla bu doğal savunma mekanizmasının bilinmesi, hastalık kontrolü için yeni stratejilerin geliştirilmesine olanak tanımaktadır. Bu çalışmada, bitki büyüme ve gelişmesinde etkili olan nitratın (NO3-) patojen enfeksiyonuna maruz kalan farklı biber genotiplerindeki tepkisi araştırılmıştır. Bunun için dirençli ve duyarlı biber genotipleri 102, 103 ve 104 zoospore/mL P. capsici-22 izolatına maruz bırakılmış ve enfeksiyondan sonraki 2., 4. ve 6. günlerde alınan kök örneklerinden NO3- içeriğindeki değişimler belirlenmiştir. Tüm zoospor konsantrasyonları, tüm günlerde CM-334'ün köklerindeki NO3- içeriğinde genel olarak bir artışa neden olmuştur. KM-181 ve SD-8 genotiplerinde en yüksek NO3- içeriği 103 zoospor/mL uygulamasının 6. gününde belirlenmiştir. SD-8 ve KM-181 genotiplerinde, 104 zoospor/mL uygulamasının 4. ve 6. günlerinde NO3- miktarında önemli bir azalma saptanmıştır. Bu genotiplerde, yüksek zoospor konsantrasyonunda enfeksiyon süresinin artışı ile birlikte NO3- miktarında azalma bulunmuştur. Üç biber genotipi karşılaştırıldığında, en yüksek NO3- içeriği, enfeksiyondan sonraki 6. günde 104 zoospor/mL'ye maruz bırakılan dirençli CM-334 genotipinde belirlenmiştir. Bu çalışmada, dirençli ve duyarlı biber genotiplerinde NO3- miktarındaki değişimler, NO3- 'ün P. capsici-22 'ye karşı bitki savunmasında etkili olabileceğini işaret etmektedir.

Keywords

Biber, nitrat, Phytophthora capsici-22

Nitrate content in roots of pepper seedlings exposed to Phytophthora capsici

Abstract

Phytophthora capsici causes root rot, a deadly plant disease. Resistance to diseases is produced by the activation of many defense substances, so knowledge of this natural defense mechanism allows the development of new strategies for disease control. In this study, the response of nitrate (NO3-), which is effective in plant growth and development, in different pepper genotypes exposed to pathogen infection was investigated. For this, resistant and sensitive pepper genotypes were exposed to 102, 103, and 104 zoospore/mL of P. capsici-22 strain and changes in NO3- content were determined from root samples taken on the 2nd, 4th and 6th days after infection. All zoospore concentrations resulted in an overall increase in NO3- content in roots of CM-334 on all days. In KM-181 and SD-8 genotypes, the highest NO3- content was determined on the 6th day of 103 zoospore/mL application. In SD-8 and KM-181 genotypes, a significant decrease in the amount of NO3- was determined on the 4th and 6th days of treatment of 104 zoospore/mL. In these genotypes, a decrease in the amount of NO3- was found with the increase in infection time at high zoospore concentration. When the three pepper genotypes were compared, the highest NO3- content was determined in the resistant CM-334 genotype, which was exposed to 104 zoospore/mL on the 6th day following the infection. In this study, changes in the amount of NO3- in resistant and susceptible pepper genotypes indicated that NO3- may be effective in plant defense against P. capsici-22.

Keywords

Nitrate, Pepper, Pthytophthora capsici- 22

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