Bisküvi örneklerinden GDO tespiti için DNA izolasyon metotlarının karşılaştırılması

Abstract

Genetiği değiştirilmiş organizmaların (GDO) kullanıldığı yem ve gıda ürünlerinin dünya çapında artış göstermesi, biyoteknolojik yollarla elde edilen bu gıdalar ile, GDO içermeyen gıdalar arasında ayrım yapabilen tespit yöntemlerinin geliştirilmesine yol açmıştır. Farklı ülkelerdeki çeşitli düzenlemelere rağmen, GDO'lu ürünlerin etiketlenmesi konusunda uluslararası düzeyde tutarlı bir yaklaşıma ihtiyaç vardır. Bu sebeple tüketicilere güvenilir ve şeffaf bir gıda güvenliği sağlamak açısından etkili GDO tespit yöntemlerinin geliştirilmesine dair ihtiyaç oluşmaktadır. GDO'ya yönelik doğru ve güvenilir tespit yöntemlerinin oluşturulmasında ilk deneysel işlem basamağı etkili bir DNA izolasyonudur. Bu konuda farklı işlenmişlik seviyesindeki gıdalara özgü DNA izolasyon yöntemlerinin belirlenmesi oldukça önemlidir. Bu çalışmada, bisküvi örnekleri için farklı DNA ekstraksiyon yöntemlerinin karşılaştırılması amaçlanmıştır. Bu sebeple farklı bisküvi örneklerine ait DNA’lar, seçilen üç faklı protokol (CTAB-PVP, modifiye CTAB-1 ve modifiye CTAB-2) kullanılarak izole edilmiştir. İşlenmiş gıda ürünleri olan bisküvilerden ekstrakte edilen DNA örnekleri, DNA konsantrasyonu ve saflığı bakımından değerlendirilmiştir. Elde edilen sonuçlara göre bisküvi örnekleri için hem DNA miktarı hem de saflık oranları bakımından genel olarak en iyi performansı CTAB-2 prosedürü göstermiştir.

Keywords

• Bisküvi
• CTAB
• DNA ekstraksiyonu
• GDO
• gıda güvenliği

Comparison of DNA isolation methods for GMO detection from biscuit samples

Abstract

The global increase in genetically modified organism (GMO) content in feed and food products has necessitated the development of precise detection methods to differentiate between biotechnologically derived foods and those without GMOs. Despite the various regulations in different countries, an internationally consistent approach to labeling GMO products is needed. For this reason, there is a widespread need to develop effective GMO detection methods to provide reliable and transparent food safety to consumers. The first experimental step in creating accurate and reliable detection methods for GMOs is effective DNA isolation. Determining DNA isolation methods specific to different processing levels of foods is very important. This study was aimed to compare different DNA extraction methods in biscuit samples. For this reason, DNA from different biscuit samples was isolated using three different protocols (CTAB-PVP, modified CTAB-1, and modified CTAB-2). DNA samples extracted from biscuits, were evaluated regarding DNA concentration and purity. According to the results obtained, the CTAB-2 procedure generally showed the best performance in terms of both DNA amount and purity rates for biscuit samples.

Keywords

• biscuit
• CTAB
• DNA extraction
• GMO
• food safety

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