Biochemical characterization of four different genotypes of Flax (Linum usitatissimum L.) seeds

Abstract

Flaxseed (Linum usitatissimum L.) is an oilseed used in industrial and natural health products. It accumulates many biologically active compounds having strong phytochemicals and antioxidant properties. This study aims to provide an entire portfolio of bioactive compounds present in four different genotypes of flaxseed (Avangard, Bony Doon, Linda and Linton). Biochemical diversity among these four different types of seed with respect to oxidant and antioxidant parameters (phenolics, flavonoids, β-karoten, lycopen, DPPH scavenging activity, MDA, proline, SOD, CAT, APx and GR) were investigated. According to results, Linda types are found to have unusual phytochemicals and antioxidant profile since its oxidative status tends to the production of oxidant molecules which was reflected in differences in radical scavenging, MDA and antioxidant enzyme systems. Other flaxseeds demonstrate noticeable activities with their antioxidant potential together with their high phytochemicals. Therefore, they may be utilized as a promising source of therapeutics since they might provide an appropriate source of antioxidants.

Keywords

• Flaxseed
• Phytochemicals
• Bioactive compounds
• Antioxidant enzymes

Dört farklı genotipe sahip keten (Linum usitatissimum L.) tohumlarının biyokimyasal karakterizasyonu

Abstract

Keten (Linum usitatissimum L.) tohumları, endüstri ve doğal sağlık ürünleri alanında kullanılan yağlı tohumlardır. Biyolojik olarak aktif pek çok fitokimyasal ve antioksidan özelliklere sahip maddeyi yapılarında bulundurabilirler. Bu çalışma, dört farklı genotipteki keten tohumlarının (Avangard, Bony Doon, Linda ve Linton) biyoaktif içeriklerinin araştırılmasını ve özetlenmesini hedeflemiştir. Buna nedenle oksidan ve antioksidan parametrelerinin (fenolikler, flavonoidler, β-karoten, likopen, DPPH radikal yakalama aktivitesi, MDA, prolin, SOD, CAT, APx ve GR) dört farklı keten tohumundaki biyokimyasal çeşitliliği araştırılmıştır. Sonuçlara göre Linda türü keten tohumlarının diğer çeşitlere oranla beklenmedik bir şekilde farklı fitokimyasal ve antioksidan profiline sahip olduğu, bu tohumların oksidatif durumlarının oksidan molekülleri üretmeye eğilimli olduğu DPPH radikal yakalama, MDA ve antioksidan enzim aktivite sonuçlarına göre belirlenmiştir. Ayrıca diğer keten tohumlarının fark edilebilir şekilde yüksek oranda antioksidan ve fitokimyasal potansiyele sahip oldukları gösterilmiştir. Bu nedenle terapötik olarak önemli özellikleri ve antioksidan içerik açısından zengin olmaları nedeniyle keten tohumlarından daha fazla faydalanılmalıdır.

Keywords

• Keten tohumu
• Fitokimyasallar
• Biyoaktif bileşikler
• Antioksidan enzimler

References

1. Ábrahám E, Hourton-Cabassa C, Erdei L, Szabados L (2010) Methods for Determination of Proline in Plants. In: Methods Mol. Biol. pp 317–331.
2. Aebi H (1984) In Methods in Enzymology. In: Catalase Vitr. Academy Press, NY, pp 121–126.
3. Amawi H, Ashby C, Samuel T, Peraman R, Tiwari AK (2017) Polyphenolic Nutrients in Cancer Chemoprevention and Metastasis: Role of the Epithelial-to-Mesenchymal (EMT) Pathway. Nutrients 9(8):911.
4. Czemplik M, Kulma A, Bazela K, Szopa J (2012) The biomedical potential of genetically modified flax seeds overexpressing the glucosyltransferase gene. BMC Complement Altern Med 12:251.
5. Foster JG, Hess JL (1980) Responses of superoxide dismutase and glutathione reductase activities in cotton leaf tissue exposed to an atmosphere enriched in oxygen. Plant Physiol 66:482–487.
6. Goyal A, Sharma V, Upadhyay N, Gill S, Sihag M (2014) Flax and flaxseed oil: an ancient medicine & modern functional food. J Food Sci Technol 51:1633–1653.
7. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275.
8. Maity P, Samanta S, Nandi AK, Sen IK, Paloi S, Acharya K, Islam SS (2014) Structure elucidation and antioxidant properties of a soluble β-d-glucan from mushroom Entoloma lividoalbum. Int J Biol Macromol 63:140–149.

9. Marklund S, Marklund G (1974) Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47:469–474.
10. Pal J, Ganguly S, Tahsin KS, Acharya K (2010) In vitro free radical scavenging activity of wild edible mushroom, Pleurotus squarrosulus (Mont.) Singer. Indian J Exp Biol 48:1210–1218.
11. Prasad K (2000) Flaxseed: a source of hypocholesterolemic and antiatherogenic agents. Drug News Perspect 13:99–104. Prasad K (2009) Flaxseed and Cardiovascular Health. J Cardiovasc Pharmacol 54:369–377.
12. Sairam RK, Saxena DC (2000) Oxidative Stress and Antioxidants in Wheat Genotypes: Possible Mechanism of Water Stress Tolerance. J Agron Crop Sci 184:55–61.
13. Savicka M, Skute N (2010) Effects of high temperature on malondialdehyde content, superoxide production and growth changes in wheat seedlings (Triticum aestivum L.). Ekologija 56:26–33.
14. Taga MS, Miller EE, Pratt DE (1984) Chia seeds as a source of natural lipid antioxidants. J Am Oil Chem Soc 61:928–931.
15. Turkoglu A, Duru ME, Mercan N, Kivrak I, Gezer K (2007) Antioxidant and antimicrobial activities of Laetiporus sulphureus (Bull.) Murrill. Food Chem 101:267–273.
16. Wang L, Chen J, Thompson LU (2005) The inhibitory effect of flaxseed on the growth and metastasisof estrogen receptor negative human breast cancer xenograftsis attributed to both its lignan and oil components. Int J Cancer 116:793–798.