Cell growth inhibitory potential of Craterellus cornucopioides (L.) Pers. together with antioxidant and antimicrobial properties

Year 2018, , 60 - 64, 01.11.2018

Sevim Kol Aykut Bostancı , Aytaç Kocabaş , Yasin Uzun , Gökhan Sadi

https://doi.org/10.30616/ajb.413645

Cited By: 2

Abstract

Craterellus cornucopioides (L.) Pers  which is also
known as trumpet of death or horn of plenty, is a wild edible macrofungus. This
study was conducted to elucidate the potential health beneficial properties of C. cornucopioides. Bioactive ingredients
(phenolics, flavonoids, β-carotene and lycopene) and DPPH radical scavenging
activities were determined. 
Additionally, cell growth inhibitory effects on HepG2 cells together
with some bacteria were evaluated. Accordingly, water and methanol extracts
contains 37.71±1.42 μg/mg and 13.78±1.60 μg/mg phenolic contents, respectively.
Similarly, methanolic extracts have higher β-caroten and lycopene content as
compared to aqueous extracts.
In parallel with these antioxidants, methanolic extracts have also higher DPPH
scavenging activity (IC₅₀: 5.26±0.67 mg/ml). Besides, water extracts
have higher flavonoid contents (2.13±0.06 μg/mg) then the methanolic
extracts.  C. cornucopioides has
also an important cell growth inhibitory effects on HepG2 cell (IC₅₀:
18.41±1.10 mg/ml for aqueous extracts and IC₅₀: 3.14±1.07 mg/ml for
methanolic extracts). Moreover, both extracts were effective on six different
bacteria tested. As a result, this study indicates that C. cornucopioides could reduce the
cellular oxidative stress because of its high antioxidant ingredients, inhibit
the growth of pathogen microrganisms and have some degree of cell growth
inhibitory potential at least to the HepG2 cells.Pers which is (L.) Craterellus cornucopioides

Keywords

Craterellus cornucopioides, antioxidant, antibacterial, cytotoxicity, HepG2

Antioksidan ve antimiktobiyal özellikleri ile birlikte Craterellus cornucopioides (L.) Pers. türünün hücre büyümesi baskılama potansiyeli

Abstract

Ölüm trompeti
veya bolluk boynuzu olarak da bilinen Craterellus
cornucopioides (L.) Pers, yenilebilir bir makrofungustur. Bu çalışma Craterellus cornucopioides (L.) Pers mantarının
sağlık açısından yararlı özelliklerini açığa çıkarmak amacıyla yapılmıştır. Çalışmada
ilgili mantarın biyoaktif içerikleri (fenolikler, flavonoidler, β-karoten ve
likopen) ve DPPH radikal süpürücü aktiviteleri belirlenmiştir. Ek olarak, HepG2
hücreleri ve bazı bakteri türleri üzrine hücre büyümesini baskılayıcı etkileri
değerlendirilmiştir. Buna göre, su ve metanol ekstraktları sırasıyla 37.71±1.42
μg/mg ve 13.78±1.60 μg/mg fenolik içeriğe sahiptir. Benzer şekilde, metanol
ekstraktları sulu ekstrelere kıyasla daha yüksek β-karoten ve likopen içeriğine
sahiptir. Bu antioksidanlara paralel olarak, metanol ekstraktları da DPPH
süpürme aktivitesine daha fazla sahiptir (ICso: 5.26±0.67 mg/ml). Ayrıca su ekstraktları,
metanol ekstraklarına göre daha yüksek flavonoid içeriğine (2.13±0.06 μg/mg)
sahiptir. C. cornucopioides, HepG2
hücresi üzerinde de önemli bir hücre büyümesi engelleyici etkiye sahiptir (IC₅₀:
su ekstratları için 18.41±1.10 mg/ml ve metanol ektraktları için IC₅₀:
3.14±1.07 mg/ml). Buna ek olarak, her iki ekstrakt da test edilen altı farklı
bakteri türü üzeine etkili olmuştur. Sonuç olarak, bu çalışma C. cornucopioides'in yüksek antioksidan
bileşenleri nedeniyle hücresel oksidatif stresi azaltabildiğini, patojen
mikroorganizmalarının büyümesini inhibe edebileceğini ve en azından HepG2
hücrelerine bir miktar hücre büyümesi inhibitör potansiyeline sahip olduğunu
göstermektedir.

Keywords

Craterellus cornucopioides, antioksidan, antibakteriyel, sitotoksisite, HepG2

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