Cell growth inhibitory potential of Craterellus cornucopioides (L.) Pers. together with antioxidant and antimicrobial properties

Abstract

Craterellus cornucopioides (L.) Pers which is also known as trumpet of death or horn of plenty, is a wild edible macrofungus. This study was conducted to elucidate the potential health beneficial properties of C. cornucopioides. Bioactive ingredients (phenolics, flavonoids, β-carotene and lycopene) and DPPH radical scavenging activities were determined. Additionally, cell growth inhibitory effects on HepG2 cells together with some bacteria were evaluated. Accordingly, water and methanol extracts contains 37.71±1.42 μg/mg and 13.78±1.60 μg/mg phenolic contents, respectively. Similarly, methanolic extracts have higher β-caroten and lycopene content as compared to aqueous extracts. In parallel with these antioxidants, methanolic extracts have also higher DPPH scavenging activity (IC50: 5.26±0.67 mg/ml). Besides, water extracts have higher flavonoid contents (2.13±0.06 μg/mg) then the methanolic extracts. C. cornucopioides has also an important cell growth inhibitory effects on HepG2 cell (IC50: 18.41±1.10 mg/ml for aqueous extracts and IC50: 3.14±1.07 mg/ml for methanolic extracts). Moreover, both extracts were effective on six different bacteria tested. As a result, this study indicates that C. cornucopioides could reduce the cellular oxidative stress because of its high antioxidant ingredients, inhibit the growth of pathogen microrganisms and have some degree of cell growth inhibitory potential at least to the HepG2 cells.

Keywords

• Craterellus cornucopioides
• antioxidant
• antibacterial
• cytotoxicity
• HepG2

Antioksidan ve antimiktobiyal özellikleri ile birlikte Craterellus cornucopioides (L.) Pers. türünün hücre büyümesi baskılama potansiyeli

Abstract

Ölüm trompeti veya bolluk boynuzu olarak da bilinen Craterellus cornucopioides (L.) Pers, yenilebilir bir makrofungustur. Bu çalışma Craterellus cornucopioides (L.) Pers mantarının sağlık açısından yararlı özelliklerini açığa çıkarmak amacıyla yapılmıştır. Çalışmada ilgili mantarın biyoaktif içerikleri (fenolikler, flavonoidler, β-karoten ve likopen) ve DPPH radikal süpürücü aktiviteleri belirlenmiştir. Ek olarak, HepG2 hücreleri ve bazı bakteri türleri üzrine hücre büyümesini baskılayıcı etkileri değerlendirilmiştir. Buna göre, su ve metanol ekstraktları sırasıyla 37.71±1.42 μg/mg ve 13.78±1.60 μg/mg fenolik içeriğe sahiptir. Benzer şekilde, metanol ekstraktları sulu ekstrelere kıyasla daha yüksek β-karoten ve likopen içeriğine sahiptir. Bu antioksidanlara paralel olarak, metanol ekstraktları da DPPH süpürme aktivitesine daha fazla sahiptir (ICso: 5.26±0.67 mg/ml). Ayrıca su ekstraktları, metanol ekstraklarına göre daha yüksek flavonoid içeriğine (2.13±0.06 μg/mg) sahiptir. C. cornucopioides, HepG2 hücresi üzerinde de önemli bir hücre büyümesi engelleyici etkiye sahiptir (IC50: su ekstratları için 18.41±1.10 mg/ml ve metanol ektraktları için IC50: 3.14±1.07 mg/ml). Buna ek olarak, her iki ekstrakt da test edilen altı farklı bakteri türü üzeine etkili olmuştur. Sonuç olarak, bu çalışma C. cornucopioides'in yüksek antioksidan bileşenleri nedeniyle hücresel oksidatif stresi azaltabildiğini, patojen mikroorganizmalarının büyümesini inhibe edebileceğini ve en azından HepG2 hücrelerine bir miktar hücre büyümesi inhibitör potansiyeline sahip olduğunu göstermektedir.

Keywords

• Craterellus cornucopioides
• antioksidan
• antibakteriyel
• sitotoksisite
• HepG2

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