Effects of Suillus collinitus (Fr.) Kuntze extracts on genotoxicity and proliferation of human lymphocytes

Year 2019, , 59 - 63, 01.11.2019

Bugrahan Emsen , Asuman Türel Yasin Uzun

https://doi.org/10.30616/ajb.605495

Cited By: 1

Abstract

Cultivated or wild edible
mushroom species have traditionally been used by humans for medical purposes
for many years. Edible mushrooms have the potential to show different
activities due to the numerous bioactive components they contain. In
particular, some mushroom species whose regulatory properties have been
identified on human immunity are of interest in the scientific world.
Considering these characteristics of edible mushroom species, in the present
study, it was examined the effects of Suillus
collinitus (Fr.) Kuntze, an important edible mushroom species, on human
peripheral lymphocytes. For this purpose, acetone and water extracts
were obtained from S. collinitus and
the effects of these extracts on genotoxicity and proliferation of human
lymphocytes were tested by chromosome aberration (CA), micronucleus (MN),
nuclear division index (NBI) and mitotic index (MI) analyses. When genotoxicity
analyses were examined, it was found that none of the tested extract
applications (1-100 mg/L) did not change the CA and MN frequencies
statistically (p > 0.05) compared
to the negative control group. Proliferation analyses showed that only the
maximum concentration (100 mg/L) application of
acetone extract of S. collinitus decreased
the NBI and MI ratio of the cells at a level of p < 0.05 compared to the negative control group.
The obtained results revealed that the acetone and water extracts of S. collinitus, especially the
applications at concentrations of 1-50 mg/L, did not show any genotoxic or
cytotoxic activity on lymphocytes involved in the human immune system.

Keywords

Cytotoxicity, Genotoxicity, Lymphocyte, Suillus collinitus

Supporting Institution

Karamanoglu Mehmetbey University Scientific Research Projects Commission

Project Number

06-YL-19

Thanks

This work was supported by Karamanoglu Mehmetbey University Scientific Research Projects Commission [grant number 06-YL-19].

Suillus collinitus (Fr.) Kuntze ekstraktlarının insan lenfositlerinin genotoksisitesi ve proliferasyonu üzerindeki etkileri

Abstract

Kültüre edilmiş veya yabani
yenilebilir mantar türleri yıllardır insanlar tarafından geleneksel olarak
tıbbi amaçlı kullanılmaktadır. Yenilebilir mantarlar içerdikleri kendilerine
özgü çok sayıda biyoaktif bileşen sayesinde farklı aktiviteler gösterme potansiyeline
sahiptir. Özellikle insan bağışıklığı üzerinde düzenleyici özellikleri tespit
edilen bazı mantar türleri bilim dünyasında dikkat çekmektedir. Yenilebilir
mantar türlerinin bu özellikleri göz önünde bulundurularak, mevcut çalışmada
önemli yenilebilir bir mantar türü olan Suillus collinitus (Fr.) Kuntze’un insan periferal
lenfositleri üzerindeki etkileri incelenmiştir. Bu amaçla, S. collinitus’tan aseton ve su ekstraktları elde
edilmiş ve bu ekstraktların insan lenfositlerinin genotoksisitesi ve proliferasyonu
üzerindeki etkileri, kromozom aberasyonu (KA), mikronükleus (MN), nükleer
bölünme indeksi (NBİ) ve mitotik indeks (Mİ) analizleri ile test edilmiştir.
Genotoksisite analizleri incelendiğinde, test edilen ekstrakt uygulamalarının
(1-100 mg/L) hiçbirinin negatif kontrol grubuna kıyasla KA
ve MN frekanslarını istatistiksel (p >
0.05) olarak değiştirmediği
tespit edilmiştir. Proliferasyon analizleri ise yalnızca S. collinitus’un aseton ekstraktının maksimum konsantrasyonlu (100
mg/L) uygulamasının, hücrelerin NBİ ve Mİ oranını negatif kontrol grubuna
kıyasla p <
0.05 düzeyinde düşürdüğünü göstermiştir. Elde
edilen sonuçlar, S. collinitus’un
aseton ve su ekstraktlarının özellikle 1-50 mg/L konsantrasyonlu
uygulamalarının, insan bağışıklık sisteminde görevli lenfositler üzerinde
herhangi bir genotoksik veya sitotoksik aktivite göstermediğini ortaya
çıkarmıştır.

Keywords

Sitotoksisite, Genotoksisite, Lenfosit, Suillus collinitus

Project Number

06-YL-19

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• Cite this article: Emsen B, Türel A, Uzun Y (2019). Effects of Suillus collinitus (Fr.) Kuntze extracts on genotoxicity and proliferation of human lymphocytes. Anatolian Journal of Botany 3(2): 59-63.